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Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation

New ecological trends and changes in consumer behavior are known to favor biofilm formation in household appliances, increasing the need for new antimicrobial materials and surfaces. Their development requires laboratory-cultivated biofilms, or biofilm model systems (BMS), which allow for accelerate...

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Autores principales: Asghari, Ehsan, Kiel, Annika, Kaltschmidt, Bernhard Peter, Wortmann, Martin, Schmidt, Nadine, Hüsgen, Bruno, Hütten, Andreas, Knabbe, Cornelius, Kaltschmidt, Christian, Kaltschmidt, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147854/
https://www.ncbi.nlm.nih.gov/pubmed/34064414
http://dx.doi.org/10.3390/microorganisms9050992
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author Asghari, Ehsan
Kiel, Annika
Kaltschmidt, Bernhard Peter
Wortmann, Martin
Schmidt, Nadine
Hüsgen, Bruno
Hütten, Andreas
Knabbe, Cornelius
Kaltschmidt, Christian
Kaltschmidt, Barbara
author_facet Asghari, Ehsan
Kiel, Annika
Kaltschmidt, Bernhard Peter
Wortmann, Martin
Schmidt, Nadine
Hüsgen, Bruno
Hütten, Andreas
Knabbe, Cornelius
Kaltschmidt, Christian
Kaltschmidt, Barbara
author_sort Asghari, Ehsan
collection PubMed
description New ecological trends and changes in consumer behavior are known to favor biofilm formation in household appliances, increasing the need for new antimicrobial materials and surfaces. Their development requires laboratory-cultivated biofilms, or biofilm model systems (BMS), which allow for accelerated growth and offer better understanding of the underlying formation mechanisms. Here, we identified bacterial strains in wildtype biofilms from a variety of materials from domestic appliances using matrix-assisted laser desorption/ionization-time of flight mass spectroscopy (MALDI-TOF-MS). Staphylococci and pseudomonads were identified by MALDI-TOF-MS as the main genera in the habitats and were analyzed for biofilm formation using various in vitro methods. Standard quantitative biofilm assays were combined with scanning electron microscopy (SEM) to characterize biofilm formation. While Pseudomonas putida, a published lead germ, was not identified in any of the collected samples, Pseudomonas aeruginosa was found to be the most dominant biofilm producer. Water-born Pseudomonads were dominantly found in compartments with water contact only, such as in detergent compartment and detergent enemata. Furthermore, materials in contact with the washing load are predominantly colonized with bacteria from the human.
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spelling pubmed-81478542021-05-26 Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation Asghari, Ehsan Kiel, Annika Kaltschmidt, Bernhard Peter Wortmann, Martin Schmidt, Nadine Hüsgen, Bruno Hütten, Andreas Knabbe, Cornelius Kaltschmidt, Christian Kaltschmidt, Barbara Microorganisms Article New ecological trends and changes in consumer behavior are known to favor biofilm formation in household appliances, increasing the need for new antimicrobial materials and surfaces. Their development requires laboratory-cultivated biofilms, or biofilm model systems (BMS), which allow for accelerated growth and offer better understanding of the underlying formation mechanisms. Here, we identified bacterial strains in wildtype biofilms from a variety of materials from domestic appliances using matrix-assisted laser desorption/ionization-time of flight mass spectroscopy (MALDI-TOF-MS). Staphylococci and pseudomonads were identified by MALDI-TOF-MS as the main genera in the habitats and were analyzed for biofilm formation using various in vitro methods. Standard quantitative biofilm assays were combined with scanning electron microscopy (SEM) to characterize biofilm formation. While Pseudomonas putida, a published lead germ, was not identified in any of the collected samples, Pseudomonas aeruginosa was found to be the most dominant biofilm producer. Water-born Pseudomonads were dominantly found in compartments with water contact only, such as in detergent compartment and detergent enemata. Furthermore, materials in contact with the washing load are predominantly colonized with bacteria from the human. MDPI 2021-05-04 /pmc/articles/PMC8147854/ /pubmed/34064414 http://dx.doi.org/10.3390/microorganisms9050992 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Asghari, Ehsan
Kiel, Annika
Kaltschmidt, Bernhard Peter
Wortmann, Martin
Schmidt, Nadine
Hüsgen, Bruno
Hütten, Andreas
Knabbe, Cornelius
Kaltschmidt, Christian
Kaltschmidt, Barbara
Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation
title Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation
title_full Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation
title_fullStr Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation
title_full_unstemmed Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation
title_short Identification of Microorganisms from Several Surfaces by MALDI-TOF MS: P. aeruginosa Is Leading in Biofilm Formation
title_sort identification of microorganisms from several surfaces by maldi-tof ms: p. aeruginosa is leading in biofilm formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147854/
https://www.ncbi.nlm.nih.gov/pubmed/34064414
http://dx.doi.org/10.3390/microorganisms9050992
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