Cargando…
Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome
Background: Obesity is an important part of polycystic ovary syndrome (PCOS) pathologies. The present study utilized the bioinformatics method to identify the molecular mechanism of obesity status in PCOS. Methods: Six transcriptome profiles of adipose tissue were obtained from online databases. The...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148487/ https://www.ncbi.nlm.nih.gov/pubmed/33910166 http://dx.doi.org/10.18632/aging.202938 |
_version_ | 1783697849748291584 |
---|---|
author | Zhou, Jiaojiao Huang, Xiaolin Xue, Bingshuang Wei, Yuhe Hua, Fei |
author_facet | Zhou, Jiaojiao Huang, Xiaolin Xue, Bingshuang Wei, Yuhe Hua, Fei |
author_sort | Zhou, Jiaojiao |
collection | PubMed |
description | Background: Obesity is an important part of polycystic ovary syndrome (PCOS) pathologies. The present study utilized the bioinformatics method to identify the molecular mechanism of obesity status in PCOS. Methods: Six transcriptome profiles of adipose tissue were obtained from online databases. The background correction and normalization were performed, and the DEGs were detected with the settings p < 0.05. The GO, KEGG pathway enrichment, and PPI network analysis were performed with the detected DEGs. Results: A total of 37 DGEs were found between obesity PCOS and healthy controls, and 8 of them were tested significant in the third database. The expression patterns of the 8 detected DGEs were then measured in another two datasets based on lean/obesity PCOS patients and healthy controls. The gene CHRDL1 was found to be in linear regression with the BMI index in PCOS patients (p = 0.0358), but such a difference was not found in healthy controls (p = 0.2487). The expression of CHRDL1 was significantly higher in obesity PCOS cases than the BMI matched healthy controls (p = 0.0415). Further enrichment research demonstrated the CHRDL1 might function as an inhibitor of the BMP4 or IGF1 signalling. Conclusion: In summary, the present study identified CHRDL1 as a candidate gene responsible for the obesity of PCOS patients. |
format | Online Article Text |
id | pubmed-8148487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-81484872021-05-26 Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome Zhou, Jiaojiao Huang, Xiaolin Xue, Bingshuang Wei, Yuhe Hua, Fei Aging (Albany NY) Research Paper Background: Obesity is an important part of polycystic ovary syndrome (PCOS) pathologies. The present study utilized the bioinformatics method to identify the molecular mechanism of obesity status in PCOS. Methods: Six transcriptome profiles of adipose tissue were obtained from online databases. The background correction and normalization were performed, and the DEGs were detected with the settings p < 0.05. The GO, KEGG pathway enrichment, and PPI network analysis were performed with the detected DEGs. Results: A total of 37 DGEs were found between obesity PCOS and healthy controls, and 8 of them were tested significant in the third database. The expression patterns of the 8 detected DGEs were then measured in another two datasets based on lean/obesity PCOS patients and healthy controls. The gene CHRDL1 was found to be in linear regression with the BMI index in PCOS patients (p = 0.0358), but such a difference was not found in healthy controls (p = 0.2487). The expression of CHRDL1 was significantly higher in obesity PCOS cases than the BMI matched healthy controls (p = 0.0415). Further enrichment research demonstrated the CHRDL1 might function as an inhibitor of the BMP4 or IGF1 signalling. Conclusion: In summary, the present study identified CHRDL1 as a candidate gene responsible for the obesity of PCOS patients. Impact Journals 2021-04-27 /pmc/articles/PMC8148487/ /pubmed/33910166 http://dx.doi.org/10.18632/aging.202938 Text en Copyright: © 2021 Zhou et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zhou, Jiaojiao Huang, Xiaolin Xue, Bingshuang Wei, Yuhe Hua, Fei Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
title | Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
title_full | Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
title_fullStr | Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
title_full_unstemmed | Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
title_short | Bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
title_sort | bioinformatics analysis of the molecular mechanism of obesity in polycystic ovary syndrome |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148487/ https://www.ncbi.nlm.nih.gov/pubmed/33910166 http://dx.doi.org/10.18632/aging.202938 |
work_keys_str_mv | AT zhoujiaojiao bioinformaticsanalysisofthemolecularmechanismofobesityinpolycysticovarysyndrome AT huangxiaolin bioinformaticsanalysisofthemolecularmechanismofobesityinpolycysticovarysyndrome AT xuebingshuang bioinformaticsanalysisofthemolecularmechanismofobesityinpolycysticovarysyndrome AT weiyuhe bioinformaticsanalysisofthemolecularmechanismofobesityinpolycysticovarysyndrome AT huafei bioinformaticsanalysisofthemolecularmechanismofobesityinpolycysticovarysyndrome |