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Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota

Bovine milk oligosaccharides (BMO) share structural similarity to selected human milk oligosaccharides, which are natural prebiotics for infants. Thus, there is a potential in including BMOs as a prebiotic in infant formula. To examine the in vivo effect of BMO-supplementation on the infant gut micr...

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Autores principales: Jakobsen, Louise Margrethe Arildsen, Sundekilde, Ulrik Kræmer, Andersen, Henrik Jørgen, Kot, Witold, Mejia, Josue Leonardo Castro, Nielsen, Dennis Sandris, Hansen, Axel Kornerup, Bertram, Hanne Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148552/
https://www.ncbi.nlm.nih.gov/pubmed/34066501
http://dx.doi.org/10.3390/microorganisms9051003
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author Jakobsen, Louise Margrethe Arildsen
Sundekilde, Ulrik Kræmer
Andersen, Henrik Jørgen
Kot, Witold
Mejia, Josue Leonardo Castro
Nielsen, Dennis Sandris
Hansen, Axel Kornerup
Bertram, Hanne Christine
author_facet Jakobsen, Louise Margrethe Arildsen
Sundekilde, Ulrik Kræmer
Andersen, Henrik Jørgen
Kot, Witold
Mejia, Josue Leonardo Castro
Nielsen, Dennis Sandris
Hansen, Axel Kornerup
Bertram, Hanne Christine
author_sort Jakobsen, Louise Margrethe Arildsen
collection PubMed
description Bovine milk oligosaccharides (BMO) share structural similarity to selected human milk oligosaccharides, which are natural prebiotics for infants. Thus, there is a potential in including BMOs as a prebiotic in infant formula. To examine the in vivo effect of BMO-supplementation on the infant gut microbiota, a BMO-rich diet (2% w/w) was fed to gnotobiotic mice (n = 11) inoculated with an infant type co-culture and compared with gnotobiotic mice receiving a control diet (n = 9). Nuclear magnetic resonance metabolomics in combination with high-throughput 16S rRNA gene amplicon sequencing was used to compare metabolic activity and microbiota composition in different compartments of the lower gastrointestinal tract. BMO components were detected in cecum and colon contents, revealing that BMO was available for the gut bacteria. The gut microbiota was dominated by Enterobacteriaceae and minor abundance of Lactobacilliaceae, while colonization of Bifidobacteriaceae did not succeed. Apart from a lower E. coli population in cecum content and lower formate (in colon) and succinate (in colon and cecum) concentrations, BMO supplementation did not result in significant changes in microbiota composition nor metabolic activity. The present study corroborates the importance of the presence of bifidobacteria for obtaining microbial-derived effects of milk oligosaccharides in the gastrointestinal tract.
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spelling pubmed-81485522021-05-26 Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota Jakobsen, Louise Margrethe Arildsen Sundekilde, Ulrik Kræmer Andersen, Henrik Jørgen Kot, Witold Mejia, Josue Leonardo Castro Nielsen, Dennis Sandris Hansen, Axel Kornerup Bertram, Hanne Christine Microorganisms Article Bovine milk oligosaccharides (BMO) share structural similarity to selected human milk oligosaccharides, which are natural prebiotics for infants. Thus, there is a potential in including BMOs as a prebiotic in infant formula. To examine the in vivo effect of BMO-supplementation on the infant gut microbiota, a BMO-rich diet (2% w/w) was fed to gnotobiotic mice (n = 11) inoculated with an infant type co-culture and compared with gnotobiotic mice receiving a control diet (n = 9). Nuclear magnetic resonance metabolomics in combination with high-throughput 16S rRNA gene amplicon sequencing was used to compare metabolic activity and microbiota composition in different compartments of the lower gastrointestinal tract. BMO components were detected in cecum and colon contents, revealing that BMO was available for the gut bacteria. The gut microbiota was dominated by Enterobacteriaceae and minor abundance of Lactobacilliaceae, while colonization of Bifidobacteriaceae did not succeed. Apart from a lower E. coli population in cecum content and lower formate (in colon) and succinate (in colon and cecum) concentrations, BMO supplementation did not result in significant changes in microbiota composition nor metabolic activity. The present study corroborates the importance of the presence of bifidobacteria for obtaining microbial-derived effects of milk oligosaccharides in the gastrointestinal tract. MDPI 2021-05-06 /pmc/articles/PMC8148552/ /pubmed/34066501 http://dx.doi.org/10.3390/microorganisms9051003 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jakobsen, Louise Margrethe Arildsen
Sundekilde, Ulrik Kræmer
Andersen, Henrik Jørgen
Kot, Witold
Mejia, Josue Leonardo Castro
Nielsen, Dennis Sandris
Hansen, Axel Kornerup
Bertram, Hanne Christine
Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
title Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
title_full Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
title_fullStr Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
title_full_unstemmed Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
title_short Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
title_sort administration of bovine milk oligosaccharide to weaning gnotobiotic mice inoculated with a simplified infant type microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148552/
https://www.ncbi.nlm.nih.gov/pubmed/34066501
http://dx.doi.org/10.3390/microorganisms9051003
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