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Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection
BACKGROUND: Antimicrobial peptides play crucial roles in organisms as the first line of defense against invading pathogens. OBJECTIVE: To isolate the hepcidin (hepc1) gene from the liver of turbot (Scophthalmus maximus) challenged with Vibrio anguillarum (GenBank accession number: AM113708), charact...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Institute of Genetic Engineering and Biotechnology
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148637/ https://www.ncbi.nlm.nih.gov/pubmed/34056028 http://dx.doi.org/10.30498/IJB.2020.2767 |
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author | Deng, Ai-Fang Jiang, Zhi-Hui Cong, Bai-Lin |
author_facet | Deng, Ai-Fang Jiang, Zhi-Hui Cong, Bai-Lin |
author_sort | Deng, Ai-Fang |
collection | PubMed |
description | BACKGROUND: Antimicrobial peptides play crucial roles in organisms as the first line of defense against invading pathogens. OBJECTIVE: To isolate the hepcidin (hepc1) gene from the liver of turbot (Scophthalmus maximus) challenged with Vibrio anguillarum (GenBank accession number: AM113708), characterize it, and assess its expression level in various tissues. MATERIALS AND METHODS: The DNA sequence of hepcidin from S. maximus was determined from the total RNA extracted and reverse transcribed from this fish. The expression levels of tissue-specific hepcidin transcripts were determined using reverse-transcriptase polymerase chain reactions. RESULTS: Hepcidin levels increased in the livers, head kidneys and spleens of the fish. The transcriptional increase was especially noticeable in the liver after bacterial infection commencement. The presence of hepcidin and interleukin-beta (IL-1β) in blood leukocytes was compared at the transcription level and hepcidin transcripts were detected earlier than IL-1β transcripts after infection, indicating that hepcidin might serve as the first line of defense to kill bacteria and may also play a more direct and effective role than that of IL-1β during the initial stage of the innate immune response when turbot are exposed to bacteria invasion. CONCLUSIONS: Hepcidin might serve as the first line of defense to kill bacteria and may also play a more direct and effective role than that of IL-1β during the initial stage of the innate immune response when turbot are exposed to bacteria invasion. |
format | Online Article Text |
id | pubmed-8148637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Institute of Genetic Engineering and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-81486372021-05-28 Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection Deng, Ai-Fang Jiang, Zhi-Hui Cong, Bai-Lin Iran J Biotechnol Research Article BACKGROUND: Antimicrobial peptides play crucial roles in organisms as the first line of defense against invading pathogens. OBJECTIVE: To isolate the hepcidin (hepc1) gene from the liver of turbot (Scophthalmus maximus) challenged with Vibrio anguillarum (GenBank accession number: AM113708), characterize it, and assess its expression level in various tissues. MATERIALS AND METHODS: The DNA sequence of hepcidin from S. maximus was determined from the total RNA extracted and reverse transcribed from this fish. The expression levels of tissue-specific hepcidin transcripts were determined using reverse-transcriptase polymerase chain reactions. RESULTS: Hepcidin levels increased in the livers, head kidneys and spleens of the fish. The transcriptional increase was especially noticeable in the liver after bacterial infection commencement. The presence of hepcidin and interleukin-beta (IL-1β) in blood leukocytes was compared at the transcription level and hepcidin transcripts were detected earlier than IL-1β transcripts after infection, indicating that hepcidin might serve as the first line of defense to kill bacteria and may also play a more direct and effective role than that of IL-1β during the initial stage of the innate immune response when turbot are exposed to bacteria invasion. CONCLUSIONS: Hepcidin might serve as the first line of defense to kill bacteria and may also play a more direct and effective role than that of IL-1β during the initial stage of the innate immune response when turbot are exposed to bacteria invasion. National Institute of Genetic Engineering and Biotechnology 2020-10-01 /pmc/articles/PMC8148637/ /pubmed/34056028 http://dx.doi.org/10.30498/IJB.2020.2767 Text en Copyright: © 2020 The Author(s); Published by Iranian Journal of Biotechnology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Deng, Ai-Fang Jiang, Zhi-Hui Cong, Bai-Lin Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection |
title | Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection |
title_full | Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection |
title_fullStr | Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection |
title_full_unstemmed | Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection |
title_short | Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection |
title_sort | hepcidin gene cloning and expression pattern in turbot (scophthalmus maximus) after vibrio. anguillarum infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148637/ https://www.ncbi.nlm.nih.gov/pubmed/34056028 http://dx.doi.org/10.30498/IJB.2020.2767 |
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