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Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways
The present study was conducted to investigate the underlying mechanisms and effective components of Polygonum hydropiper in ethanol-induced acute gastric mucosal lesions. The ethanol extract was purified on an AB-8 macroporous resin column and eluted with 60% ethanol and was then injected into the...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Associação Brasileira de Divulgação Científica
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148888/ https://www.ncbi.nlm.nih.gov/pubmed/34037095 http://dx.doi.org/10.1590/1414-431X2020e10841 |
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author | Ren, Shouzhong Chen, Bangpei Ma, Zhijian Hu, Hui Xie, Yiqiang |
author_facet | Ren, Shouzhong Chen, Bangpei Ma, Zhijian Hu, Hui Xie, Yiqiang |
author_sort | Ren, Shouzhong |
collection | PubMed |
description | The present study was conducted to investigate the underlying mechanisms and effective components of Polygonum hydropiper in ethanol-induced acute gastric mucosal lesions. The ethanol extract was purified on an AB-8 macroporous resin column and eluted with 60% ethanol and was then injected into the HPLC system for quantitative analysis. Sprague-Dawley rats were orally pretreated with P. hydropiper extract (PHLE; 50, 100, and 200 mg/kg) for 5 days and then absolute ethanol was administered to induce gastric mucosal damage. One hour after ethanol ingestion, the rats were euthanized and stomach samples were collected for biochemical analysis. Antioxidant enzymes and anti-inflammatory cytokines were quantified. Western blotting was used to detect the expression levels of proteins. Cell proliferation was assayed by CCK-8 assays. The proportion of total flavonoids in the final extract of P. hydropiper was 50.05%, which contained three major bioactive flavonoid constituents, including rutin, quercitrin, and quercetin. PHLE significantly increased cell viability and effectively protected human gastric epithelial cells-1 against alcohol-induced damage in vitro. PHLE pretreatment attenuated gastric mucosal injuries in a dose-dependent manner in rats, and increased the activity of superoxide dismutase, glutathione peroxidase, and glutathione, and decreased the levels of malondialdehyde in gastric tissue. Pretreatment with PHLE also reduced the generation of the pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β in gastric tissue by downregulating the expression of nuclear factor-kappa B. PHLE exerted protective effects against gastric injury through antioxidant and anti-inflammatory pathways. Flavonoids might be the main effective components of P. hydropiper against gastric mucosal injury. |
format | Online Article Text |
id | pubmed-8148888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-81488882021-06-04 Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways Ren, Shouzhong Chen, Bangpei Ma, Zhijian Hu, Hui Xie, Yiqiang Braz J Med Biol Res Research Article The present study was conducted to investigate the underlying mechanisms and effective components of Polygonum hydropiper in ethanol-induced acute gastric mucosal lesions. The ethanol extract was purified on an AB-8 macroporous resin column and eluted with 60% ethanol and was then injected into the HPLC system for quantitative analysis. Sprague-Dawley rats were orally pretreated with P. hydropiper extract (PHLE; 50, 100, and 200 mg/kg) for 5 days and then absolute ethanol was administered to induce gastric mucosal damage. One hour after ethanol ingestion, the rats were euthanized and stomach samples were collected for biochemical analysis. Antioxidant enzymes and anti-inflammatory cytokines were quantified. Western blotting was used to detect the expression levels of proteins. Cell proliferation was assayed by CCK-8 assays. The proportion of total flavonoids in the final extract of P. hydropiper was 50.05%, which contained three major bioactive flavonoid constituents, including rutin, quercitrin, and quercetin. PHLE significantly increased cell viability and effectively protected human gastric epithelial cells-1 against alcohol-induced damage in vitro. PHLE pretreatment attenuated gastric mucosal injuries in a dose-dependent manner in rats, and increased the activity of superoxide dismutase, glutathione peroxidase, and glutathione, and decreased the levels of malondialdehyde in gastric tissue. Pretreatment with PHLE also reduced the generation of the pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β in gastric tissue by downregulating the expression of nuclear factor-kappa B. PHLE exerted protective effects against gastric injury through antioxidant and anti-inflammatory pathways. Flavonoids might be the main effective components of P. hydropiper against gastric mucosal injury. Associação Brasileira de Divulgação Científica 2021-05-24 /pmc/articles/PMC8148888/ /pubmed/34037095 http://dx.doi.org/10.1590/1414-431X2020e10841 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ren, Shouzhong Chen, Bangpei Ma, Zhijian Hu, Hui Xie, Yiqiang Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways |
title |
Polygonum hydropiper extract attenuates ethanol-induced gastric
damage through antioxidant and anti-inflammatory pathways |
title_full |
Polygonum hydropiper extract attenuates ethanol-induced gastric
damage through antioxidant and anti-inflammatory pathways |
title_fullStr |
Polygonum hydropiper extract attenuates ethanol-induced gastric
damage through antioxidant and anti-inflammatory pathways |
title_full_unstemmed |
Polygonum hydropiper extract attenuates ethanol-induced gastric
damage through antioxidant and anti-inflammatory pathways |
title_short |
Polygonum hydropiper extract attenuates ethanol-induced gastric
damage through antioxidant and anti-inflammatory pathways |
title_sort | polygonum hydropiper extract attenuates ethanol-induced gastric
damage through antioxidant and anti-inflammatory pathways |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148888/ https://www.ncbi.nlm.nih.gov/pubmed/34037095 http://dx.doi.org/10.1590/1414-431X2020e10841 |
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