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Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis

Mucosal healing comprises a key goal of ulcerative colitis (UC) treatment. Anterior gradient protein 2 (AGR2) plays an important role in maintaining intestinal homeostasis in UC. However, the role of AGR2 in the repair of mucosal injury is not yet clear. This study is aimed at investigating the expr...

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Autores principales: Ye, Xiaolin, Wu, Jie, Li, Jing, Wang, Hongyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149229/
https://www.ncbi.nlm.nih.gov/pubmed/34055987
http://dx.doi.org/10.1155/2021/6483860
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author Ye, Xiaolin
Wu, Jie
Li, Jing
Wang, Hongyu
author_facet Ye, Xiaolin
Wu, Jie
Li, Jing
Wang, Hongyu
author_sort Ye, Xiaolin
collection PubMed
description Mucosal healing comprises a key goal of ulcerative colitis (UC) treatment. Anterior gradient protein 2 (AGR2) plays an important role in maintaining intestinal homeostasis in UC. However, the role of AGR2 in the repair of mucosal injury is not yet clear. This study is aimed at investigating the expression of AGR2 in the intestinal tissues of children with UC and its role in repairing mucosal injury. Forty UC patients who were hospitalized in the Pediatric Gastroenterology Ward of Shengjing Hospital affiliated with China Medical University between July 1, 2013, and May 31, 2020, and 20 children who had normal colonoscopy results during the same period (control group) made up the study sample. The disease activity of UC was evaluated based on the pediatric ulcerative colitis activity index, and the ulcerative colitis endoscopic index was evaluated according to the Rachmilewitz score. Immunohistochemical staining was employed to examine the differences in AGR2 expression in the intestinal mucosa between groups. The protective effect of AGR2 in a model of tumor necrosis factor-alpha- (TNF-α-) induced intestinal mucosal barrier injury and the underlying molecular mechanism were explored through in vitro experiments. The results showed that compared with the normal control group, UC patients in the remission or active period had significantly higher expression of AGR2 in the intestine. AGR2 expression was positively correlated with Ki67, an intestinal epithelial cell proliferation marker, but negatively correlated with the degree of endoscopic mucosal injury. In an in vitro model, AGR2 overexpression promoted cell proliferation and migration and inhibited TNF-α-induced intestinal epithelial barrier damage by activating yes-associated protein (YAP). Collectively, our study suggests that AGR2 might serve as a valuable biomarker to help assess the condition and mucosal healing status of UC patients. In vitro, AGR2 promoted the repair of intestinal mucosal barrier injury by activating YAP.
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spelling pubmed-81492292021-05-27 Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis Ye, Xiaolin Wu, Jie Li, Jing Wang, Hongyu Biomed Res Int Research Article Mucosal healing comprises a key goal of ulcerative colitis (UC) treatment. Anterior gradient protein 2 (AGR2) plays an important role in maintaining intestinal homeostasis in UC. However, the role of AGR2 in the repair of mucosal injury is not yet clear. This study is aimed at investigating the expression of AGR2 in the intestinal tissues of children with UC and its role in repairing mucosal injury. Forty UC patients who were hospitalized in the Pediatric Gastroenterology Ward of Shengjing Hospital affiliated with China Medical University between July 1, 2013, and May 31, 2020, and 20 children who had normal colonoscopy results during the same period (control group) made up the study sample. The disease activity of UC was evaluated based on the pediatric ulcerative colitis activity index, and the ulcerative colitis endoscopic index was evaluated according to the Rachmilewitz score. Immunohistochemical staining was employed to examine the differences in AGR2 expression in the intestinal mucosa between groups. The protective effect of AGR2 in a model of tumor necrosis factor-alpha- (TNF-α-) induced intestinal mucosal barrier injury and the underlying molecular mechanism were explored through in vitro experiments. The results showed that compared with the normal control group, UC patients in the remission or active period had significantly higher expression of AGR2 in the intestine. AGR2 expression was positively correlated with Ki67, an intestinal epithelial cell proliferation marker, but negatively correlated with the degree of endoscopic mucosal injury. In an in vitro model, AGR2 overexpression promoted cell proliferation and migration and inhibited TNF-α-induced intestinal epithelial barrier damage by activating yes-associated protein (YAP). Collectively, our study suggests that AGR2 might serve as a valuable biomarker to help assess the condition and mucosal healing status of UC patients. In vitro, AGR2 promoted the repair of intestinal mucosal barrier injury by activating YAP. Hindawi 2021-05-18 /pmc/articles/PMC8149229/ /pubmed/34055987 http://dx.doi.org/10.1155/2021/6483860 Text en Copyright © 2021 Xiaolin Ye et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ye, Xiaolin
Wu, Jie
Li, Jing
Wang, Hongyu
Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis
title Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis
title_full Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis
title_fullStr Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis
title_full_unstemmed Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis
title_short Anterior Gradient Protein 2 Promotes Mucosal Repair in Pediatric Ulcerative Colitis
title_sort anterior gradient protein 2 promotes mucosal repair in pediatric ulcerative colitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149229/
https://www.ncbi.nlm.nih.gov/pubmed/34055987
http://dx.doi.org/10.1155/2021/6483860
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