Modular barcode beads for microfluidic single cell genomics

Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is obtained is possible but the result is suboptimal....

Descripción completa

Detalles Bibliográficos
Autores principales: Delley, Cyrille L., Abate, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149635/
https://www.ncbi.nlm.nih.gov/pubmed/34035349
http://dx.doi.org/10.1038/s41598-021-90255-x
_version_ 1783697987879305216
author Delley, Cyrille L.
Abate, Adam R.
author_facet Delley, Cyrille L.
Abate, Adam R.
author_sort Delley, Cyrille L.
collection PubMed
description Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is obtained is possible but the result is suboptimal. Here, we describe a modular framework that simplifies generation of multifunctional beads and allows their easy extension to new targets.
format Online
Article
Text
id pubmed-8149635
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-81496352021-05-26 Modular barcode beads for microfluidic single cell genomics Delley, Cyrille L. Abate, Adam R. Sci Rep Article Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is obtained is possible but the result is suboptimal. Here, we describe a modular framework that simplifies generation of multifunctional beads and allows their easy extension to new targets. Nature Publishing Group UK 2021-05-25 /pmc/articles/PMC8149635/ /pubmed/34035349 http://dx.doi.org/10.1038/s41598-021-90255-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Delley, Cyrille L.
Abate, Adam R.
Modular barcode beads for microfluidic single cell genomics
title Modular barcode beads for microfluidic single cell genomics
title_full Modular barcode beads for microfluidic single cell genomics
title_fullStr Modular barcode beads for microfluidic single cell genomics
title_full_unstemmed Modular barcode beads for microfluidic single cell genomics
title_short Modular barcode beads for microfluidic single cell genomics
title_sort modular barcode beads for microfluidic single cell genomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149635/
https://www.ncbi.nlm.nih.gov/pubmed/34035349
http://dx.doi.org/10.1038/s41598-021-90255-x
work_keys_str_mv AT delleycyrillel modularbarcodebeadsformicrofluidicsinglecellgenomics
AT abateadamr modularbarcodebeadsformicrofluidicsinglecellgenomics

Ejemplares similares