Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics

Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the...

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Autores principales: Lin, Ruizhe, Kipreos, Edward T., Zhu, Jie, Khang, Chang Hyun, Kner, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149693/
https://www.ncbi.nlm.nih.gov/pubmed/34035309
http://dx.doi.org/10.1038/s41467-021-23449-6
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author Lin, Ruizhe
Kipreos, Edward T.
Zhu, Jie
Khang, Chang Hyun
Kner, Peter
author_facet Lin, Ruizhe
Kipreos, Edward T.
Zhu, Jie
Khang, Chang Hyun
Kner, Peter
author_sort Lin, Ruizhe
collection PubMed
description Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples.
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spelling pubmed-81496932021-06-01 Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics Lin, Ruizhe Kipreos, Edward T. Zhu, Jie Khang, Chang Hyun Kner, Peter Nat Commun Article Structured Illumination Microscopy enables live imaging with sub-diffraction resolution. Unfortunately, optical aberrations can lead to loss of resolution and artifacts in Structured Illumination Microscopy rendering the technique unusable in samples thicker than a single cell. Here we report on the combination of Adaptive Optics and Structured Illumination Microscopy enabling imaging with 150 nm lateral and 570 nm axial resolution at a depth of 80 µm through Caenorhabditis elegans. We demonstrate that Adaptive Optics improves the three-dimensional resolution, especially along the axial direction, and reduces artifacts, successfully realizing 3D-Structured Illumination Microscopy in a variety of biological samples. Nature Publishing Group UK 2021-05-25 /pmc/articles/PMC8149693/ /pubmed/34035309 http://dx.doi.org/10.1038/s41467-021-23449-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lin, Ruizhe
Kipreos, Edward T.
Zhu, Jie
Khang, Chang Hyun
Kner, Peter
Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_full Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_fullStr Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_full_unstemmed Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_short Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
title_sort subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149693/
https://www.ncbi.nlm.nih.gov/pubmed/34035309
http://dx.doi.org/10.1038/s41467-021-23449-6
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