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The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides

Nutritional Programming (NP) has been studied as a means of improving dietary plant protein (PP) utilization in different fish species. This study investigated the use of enriched live feed as a vehicle for NP in larval fish. The objective of this study was to determine the effect of NP induced duri...

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Autores principales: Molinari, Giovanni S., Wojno, Michal, Kwasek, Karolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149696/
https://www.ncbi.nlm.nih.gov/pubmed/34035318
http://dx.doi.org/10.1038/s41598-021-89803-2
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author Molinari, Giovanni S.
Wojno, Michal
Kwasek, Karolina
author_facet Molinari, Giovanni S.
Wojno, Michal
Kwasek, Karolina
author_sort Molinari, Giovanni S.
collection PubMed
description Nutritional Programming (NP) has been studied as a means of improving dietary plant protein (PP) utilization in different fish species. This study investigated the use of enriched live feed as a vehicle for NP in larval fish. The objective of this study was to determine the effect of NP induced during the larval stage via PP-enriched live feed on: (1) growth performance; (2) expression of genes associated with inflammation and any morphological changes in the intestine; and (3) muscle free amino acid composition in largemouth bass (Micropterus salmoides) during its later life stages. Two diets were used in this study, a fish meal (FM)-based diet, and a soybean mean (SBM)-based diet, serving as the PP diet. There were 4 groups in this study. The two control groups, ( +) Control and (−) Control, were not programmed and received the FM-diet and SBM-diet, respectively throughout the whole trial after the live feed stage (27–122 days post hatch (dph). The next group, programmed, was programmed with SBM-enriched Artemia nauplii during the live feed stage (4–26 dph) and challenged with the SBM-diet during the final stage of the study (79–122 dph). The final group, non-programmed, did not receive any programming and, was challenged with the SBM-diet during the final stage of the study. The programmed group experienced a significantly higher (%) weight gain during the PP-Challenge than the non-programmed group. In addition, the live feed programming resulted in significantly longer distal villi, and a higher villi length to width ratio, compared to the non-programmed group. No significant effects on free amino acid composition and gene expression were observed between the programmed and non-programmed group, except for an increased post-prandial concentration of free proline in the programmed group. The results of this study support use of live feed as a vehicle for nutritional programming and improving the growth performance of largemouth bass fed with a SBM-based diet.
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spelling pubmed-81496962021-05-26 The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides Molinari, Giovanni S. Wojno, Michal Kwasek, Karolina Sci Rep Article Nutritional Programming (NP) has been studied as a means of improving dietary plant protein (PP) utilization in different fish species. This study investigated the use of enriched live feed as a vehicle for NP in larval fish. The objective of this study was to determine the effect of NP induced during the larval stage via PP-enriched live feed on: (1) growth performance; (2) expression of genes associated with inflammation and any morphological changes in the intestine; and (3) muscle free amino acid composition in largemouth bass (Micropterus salmoides) during its later life stages. Two diets were used in this study, a fish meal (FM)-based diet, and a soybean mean (SBM)-based diet, serving as the PP diet. There were 4 groups in this study. The two control groups, ( +) Control and (−) Control, were not programmed and received the FM-diet and SBM-diet, respectively throughout the whole trial after the live feed stage (27–122 days post hatch (dph). The next group, programmed, was programmed with SBM-enriched Artemia nauplii during the live feed stage (4–26 dph) and challenged with the SBM-diet during the final stage of the study (79–122 dph). The final group, non-programmed, did not receive any programming and, was challenged with the SBM-diet during the final stage of the study. The programmed group experienced a significantly higher (%) weight gain during the PP-Challenge than the non-programmed group. In addition, the live feed programming resulted in significantly longer distal villi, and a higher villi length to width ratio, compared to the non-programmed group. No significant effects on free amino acid composition and gene expression were observed between the programmed and non-programmed group, except for an increased post-prandial concentration of free proline in the programmed group. The results of this study support use of live feed as a vehicle for nutritional programming and improving the growth performance of largemouth bass fed with a SBM-based diet. Nature Publishing Group UK 2021-05-25 /pmc/articles/PMC8149696/ /pubmed/34035318 http://dx.doi.org/10.1038/s41598-021-89803-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Molinari, Giovanni S.
Wojno, Michal
Kwasek, Karolina
The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
title The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
title_full The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
title_fullStr The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
title_full_unstemmed The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
title_short The use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass Micropterus salmoides
title_sort use of live food as a vehicle of soybean meal for nutritional programming of largemouth bass micropterus salmoides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149696/
https://www.ncbi.nlm.nih.gov/pubmed/34035318
http://dx.doi.org/10.1038/s41598-021-89803-2
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