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A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture

Compared with endophytes, metabolites from endophytes (MEs) have great potential in agriculture. However, a technique for industrializing the production of MEs is still scarce. Moreover, the establishment of effective methods for evaluating the quality of MEs is hampered by the fact that some compou...

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Autores principales: Wang, Qingbin, Peng, Chune, Shi, Liran, Liu, Zhiguang, Zhou, Dafa, Meng, Hui, Zhao, Hongling, Li, Fuchuan, Zhang, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149806/
https://www.ncbi.nlm.nih.gov/pubmed/34055763
http://dx.doi.org/10.3389/fbioe.2021.671879
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author Wang, Qingbin
Peng, Chune
Shi, Liran
Liu, Zhiguang
Zhou, Dafa
Meng, Hui
Zhao, Hongling
Li, Fuchuan
Zhang, Min
author_facet Wang, Qingbin
Peng, Chune
Shi, Liran
Liu, Zhiguang
Zhou, Dafa
Meng, Hui
Zhao, Hongling
Li, Fuchuan
Zhang, Min
author_sort Wang, Qingbin
collection PubMed
description Compared with endophytes, metabolites from endophytes (MEs) have great potential in agriculture. However, a technique for industrializing the production of MEs is still scarce. Moreover, the establishment of effective methods for evaluating the quality of MEs is hampered by the fact that some compounds with beneficial effects on crops have not been clearly identified. Herein, a system was established for the production, quality control and application of MEs by using the extract from Paecilomyces variotii SJ1 (ZNC). First, the extraction conditions of ZNC were optimized through response surface methodology, after which each batch (500 L) met the consumption requirements of crops in 7,467 hectares. Then, chromatographic fingerprinting and enzyme-linked immunosorbent assay were applied to evaluate the similarity and specificity of unknown effective components in ZNC, ensuring a similarity of more than 90% and a quantitative accuracy of greater than 99.9% for the products from different batches. Finally, the bioactivity of industrially produced ZNC was evaluated in the field, and it significantly increased the potato yields by 4.4–10.8%. Overall, we have established a practical technical system for the large-scale application of ZNC in agriculture.
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spelling pubmed-81498062021-05-27 A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture Wang, Qingbin Peng, Chune Shi, Liran Liu, Zhiguang Zhou, Dafa Meng, Hui Zhao, Hongling Li, Fuchuan Zhang, Min Front Bioeng Biotechnol Bioengineering and Biotechnology Compared with endophytes, metabolites from endophytes (MEs) have great potential in agriculture. However, a technique for industrializing the production of MEs is still scarce. Moreover, the establishment of effective methods for evaluating the quality of MEs is hampered by the fact that some compounds with beneficial effects on crops have not been clearly identified. Herein, a system was established for the production, quality control and application of MEs by using the extract from Paecilomyces variotii SJ1 (ZNC). First, the extraction conditions of ZNC were optimized through response surface methodology, after which each batch (500 L) met the consumption requirements of crops in 7,467 hectares. Then, chromatographic fingerprinting and enzyme-linked immunosorbent assay were applied to evaluate the similarity and specificity of unknown effective components in ZNC, ensuring a similarity of more than 90% and a quantitative accuracy of greater than 99.9% for the products from different batches. Finally, the bioactivity of industrially produced ZNC was evaluated in the field, and it significantly increased the potato yields by 4.4–10.8%. Overall, we have established a practical technical system for the large-scale application of ZNC in agriculture. Frontiers Media S.A. 2021-05-12 /pmc/articles/PMC8149806/ /pubmed/34055763 http://dx.doi.org/10.3389/fbioe.2021.671879 Text en Copyright © 2021 Wang, Peng, Shi, Liu, Zhou, Meng, Zhao, Li and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wang, Qingbin
Peng, Chune
Shi, Liran
Liu, Zhiguang
Zhou, Dafa
Meng, Hui
Zhao, Hongling
Li, Fuchuan
Zhang, Min
A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
title A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
title_full A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
title_fullStr A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
title_full_unstemmed A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
title_short A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
title_sort technical system for the large-scale application of metabolites from paecilomyces variotii sj1 in agriculture
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149806/
https://www.ncbi.nlm.nih.gov/pubmed/34055763
http://dx.doi.org/10.3389/fbioe.2021.671879
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