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Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice

OBJECTIVE: Obesity-related diseases such as diabetes, hypertension, dyslipidemia, and cardiovascular diseases have increased due to the obesity epidemic. Early intervention for obesity through lifestyle and nutrition plays an important role in preventing obesity-related diseases. Therefore, the purp...

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Autores principales: Jiang, Xiaofan, Zhang, Yuwei, Hu, Weichao, Liang, Yuxiu, Zheng, Liang, Zheng, Juan, Wang, Baozhen, Guo, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150005/
https://www.ncbi.nlm.nih.gov/pubmed/34054726
http://dx.doi.org/10.3389/fendo.2021.651303
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author Jiang, Xiaofan
Zhang, Yuwei
Hu, Weichao
Liang, Yuxiu
Zheng, Liang
Zheng, Juan
Wang, Baozhen
Guo, Xin
author_facet Jiang, Xiaofan
Zhang, Yuwei
Hu, Weichao
Liang, Yuxiu
Zheng, Liang
Zheng, Juan
Wang, Baozhen
Guo, Xin
author_sort Jiang, Xiaofan
collection PubMed
description OBJECTIVE: Obesity-related diseases such as diabetes, hypertension, dyslipidemia, and cardiovascular diseases have increased due to the obesity epidemic. Early intervention for obesity through lifestyle and nutrition plays an important role in preventing obesity-related diseases. Therefore, the purpose of this study is to explore the role of leucine and exercise in adiposity, systemic insulin resistance, and inflammation to provide theoretical and guiding basis for the early prevention and treatment of obesity. METHODS: C57BL/6J male mice were randomly divided into HFD or LFD-fed mice group. After 9 weeks, glucose tolerance test (GTT) was performed to detect their systemic insulin sensitivity. Starting from week 10, mice were divided into eight groups and treated with moderate exercise or/and 1.5% leucine. At week 13, systemic insulin sensitivity was detected by GTT. At week 14, mice were dissected to analyze adiposity and inflammation. RESULTS: In LFD mice, exercise significantly increased systemic insulin sensitivity by increasing GLUT4 expression in the muscle and decreasing adiposity through increasing AMPK phosphorylation in adipose tissue. In HFD mice, the simultaneous intervention of exercise and leucine increases systemic insulin sensitivity by reducing liver and adipose tissue inflammation via decreasing NF-κB p65 phosphorylation, and increasing the expression of adiponectin in adipose tissue. CONCLUSION: There are different mechanisms underlying the effects of exercise and leucine on insulin resistance and inflammation in LFD-fed mice or HFD-fed mice.
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spelling pubmed-81500052021-05-27 Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice Jiang, Xiaofan Zhang, Yuwei Hu, Weichao Liang, Yuxiu Zheng, Liang Zheng, Juan Wang, Baozhen Guo, Xin Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: Obesity-related diseases such as diabetes, hypertension, dyslipidemia, and cardiovascular diseases have increased due to the obesity epidemic. Early intervention for obesity through lifestyle and nutrition plays an important role in preventing obesity-related diseases. Therefore, the purpose of this study is to explore the role of leucine and exercise in adiposity, systemic insulin resistance, and inflammation to provide theoretical and guiding basis for the early prevention and treatment of obesity. METHODS: C57BL/6J male mice were randomly divided into HFD or LFD-fed mice group. After 9 weeks, glucose tolerance test (GTT) was performed to detect their systemic insulin sensitivity. Starting from week 10, mice were divided into eight groups and treated with moderate exercise or/and 1.5% leucine. At week 13, systemic insulin sensitivity was detected by GTT. At week 14, mice were dissected to analyze adiposity and inflammation. RESULTS: In LFD mice, exercise significantly increased systemic insulin sensitivity by increasing GLUT4 expression in the muscle and decreasing adiposity through increasing AMPK phosphorylation in adipose tissue. In HFD mice, the simultaneous intervention of exercise and leucine increases systemic insulin sensitivity by reducing liver and adipose tissue inflammation via decreasing NF-κB p65 phosphorylation, and increasing the expression of adiponectin in adipose tissue. CONCLUSION: There are different mechanisms underlying the effects of exercise and leucine on insulin resistance and inflammation in LFD-fed mice or HFD-fed mice. Frontiers Media S.A. 2021-05-12 /pmc/articles/PMC8150005/ /pubmed/34054726 http://dx.doi.org/10.3389/fendo.2021.651303 Text en Copyright © 2021 Jiang, Zhang, Hu, Liang, Zheng, Zheng, Wang and Guo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Jiang, Xiaofan
Zhang, Yuwei
Hu, Weichao
Liang, Yuxiu
Zheng, Liang
Zheng, Juan
Wang, Baozhen
Guo, Xin
Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice
title Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice
title_full Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice
title_fullStr Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice
title_full_unstemmed Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice
title_short Different Effects of Leucine Supplementation and/or Exercise on Systemic Insulin Sensitivity in Mice
title_sort different effects of leucine supplementation and/or exercise on systemic insulin sensitivity in mice
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150005/
https://www.ncbi.nlm.nih.gov/pubmed/34054726
http://dx.doi.org/10.3389/fendo.2021.651303
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