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Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay
Rapid and simple serologic tests that require only a small amount of blood without the euthanization of animals are valuable for microbial control in colonies of laboratory animals. In this study, we developed a multiplex immunochromatographic assay (ICA) for detection of antibodies to Sendai virus...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150241/ https://www.ncbi.nlm.nih.gov/pubmed/33177250 http://dx.doi.org/10.1538/expanim.20-0099 |
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author | Tosa, Noriko Ishida, Tomoko Yoshimatsu, Kumiko Hayashimoto, Nobuhito Shiokawa, Kanae Takakura, Akira Arikawa, Jiro |
author_facet | Tosa, Noriko Ishida, Tomoko Yoshimatsu, Kumiko Hayashimoto, Nobuhito Shiokawa, Kanae Takakura, Akira Arikawa, Jiro |
author_sort | Tosa, Noriko |
collection | PubMed |
description | Rapid and simple serologic tests that require only a small amount of blood without the euthanization of animals are valuable for microbial control in colonies of laboratory animals. In this study, we developed a multiplex immunochromatographic assay (ICA) for detection of antibodies to Sendai virus (also known as hemagglutinating virus of Japan), hantavirus, and sialodacryoadenitis virus, which are causative agents of major infectious diseases in rats. For this assay, an ICA strip was placed into a microtube containing 150 µl PBS and either 0.75 µl of rat serum or 1.5 µl of whole blood. Binding antibodies were visualized by using anti-rat IgG antibody-conjugated colloidal gold. Under these conditions, the multiplex ICA simultaneously and specifically detected antibodies to multiple antigens. Positive serum samples for each infectious disease were used to evaluate the sensitivity and specificity of the multiplex ICA. The sensitivities of the multiplex ICA for Sendai virus, hantavirus, and sialodacryoadenitis virus were 100%, 100%, and 81%, respectively. No nonspecific reactions were observed in any of the 52 positive sera against heterologous antigens. In addition, 10 samples of uninfected sera did not show any bands except for the control line. These observations indicate high specificity of the multiplex ICA. Moreover, the multiplex ICA could be applied to diluted blood. These results indicate that the multiplex ICA is appropriate for rapid and simple serological testing of laboratory rats. |
format | Online Article Text |
id | pubmed-8150241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Association for Laboratory Animal Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81502412021-05-28 Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay Tosa, Noriko Ishida, Tomoko Yoshimatsu, Kumiko Hayashimoto, Nobuhito Shiokawa, Kanae Takakura, Akira Arikawa, Jiro Exp Anim Original Rapid and simple serologic tests that require only a small amount of blood without the euthanization of animals are valuable for microbial control in colonies of laboratory animals. In this study, we developed a multiplex immunochromatographic assay (ICA) for detection of antibodies to Sendai virus (also known as hemagglutinating virus of Japan), hantavirus, and sialodacryoadenitis virus, which are causative agents of major infectious diseases in rats. For this assay, an ICA strip was placed into a microtube containing 150 µl PBS and either 0.75 µl of rat serum or 1.5 µl of whole blood. Binding antibodies were visualized by using anti-rat IgG antibody-conjugated colloidal gold. Under these conditions, the multiplex ICA simultaneously and specifically detected antibodies to multiple antigens. Positive serum samples for each infectious disease were used to evaluate the sensitivity and specificity of the multiplex ICA. The sensitivities of the multiplex ICA for Sendai virus, hantavirus, and sialodacryoadenitis virus were 100%, 100%, and 81%, respectively. No nonspecific reactions were observed in any of the 52 positive sera against heterologous antigens. In addition, 10 samples of uninfected sera did not show any bands except for the control line. These observations indicate high specificity of the multiplex ICA. Moreover, the multiplex ICA could be applied to diluted blood. These results indicate that the multiplex ICA is appropriate for rapid and simple serological testing of laboratory rats. Japanese Association for Laboratory Animal Science 2020-11-12 2021 /pmc/articles/PMC8150241/ /pubmed/33177250 http://dx.doi.org/10.1538/expanim.20-0099 Text en ©2021 Japanese Association for Laboratory Animal Science https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Tosa, Noriko Ishida, Tomoko Yoshimatsu, Kumiko Hayashimoto, Nobuhito Shiokawa, Kanae Takakura, Akira Arikawa, Jiro Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
title | Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
title_full | Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
title_fullStr | Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
title_full_unstemmed | Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
title_short | Simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
title_sort | simultaneous serodetection of major rat infectious pathogens by a multiplex immunochromatographic assay |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150241/ https://www.ncbi.nlm.nih.gov/pubmed/33177250 http://dx.doi.org/10.1538/expanim.20-0099 |
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