Cargando…

Expression and purification of recombinant SARS-CoV-2 nucleocapsid protein in inclusion bodies and its application in serological detection

The current standard for the diagnosis of COVID-19 is the nucleic acid test of SARS-CoV-2 RNA, however, virus antibody detection has the advantages of convenient sample collection, high throughout, and low cost. When combining detection with nucleic acid detection, antibody detection can effectively...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Guang, Li, Weiping, Fang, Xiaolan, Song, Xuri, Teng, Shujing, Ren, Zong, Hu, Daoqi, Zhou, Songhui, Wu, Gangqiang, Li, Keqiang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Inc. 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150265/ https://www.ncbi.nlm.nih.gov/pubmed/34048905 http://dx.doi.org/10.1016/j.pep.2021.105908

Ejemplares similares

Affinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body
por: Seyedinkhorasani, Masoud, et al.
Publicado: (2020)

Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests
por: Takano, Tomomi, et al.
Publicado: (2014)

Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2
por: Djukic, Teodora, et al.
Publicado: (2021)

Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein
por: Upadhyay, Arun K., et al.
Publicado: (2014)

Engineered recombinant protein products of the avian paramyxovirus type-1 nucleocapsid and phosphoprotein genes for serological diagnosis
por: Zhao, Na, et al.
Publicado: (2018)

Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS
por: Saijo, Masayuki, et al.
Publicado: (2005)

Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
por: JUNNU, Sucheeva, et al.
Publicado: (2013)

Cloning, Expression, and Purification of the Nucleocapsid Protein of SARS Coronavirus
por: Netesova, N. A., et al.
Publicado: (2004)

Solubilization and purification of recombinant modified C-reactive protein from inclusion bodies using reversible anhydride modification
por: Potempa, Lawrence A., et al.
Publicado: (2015)

Recombinant SARS-CoV-2 Nucleocapsid Protein: Expression, Purification, and Its Biochemical Characterization and Utility in Serological Assay Development to Assess Immunological Responses to SARS-CoV-2 Infection
por: Di, Da, et al.
Publicado: (2021)

Purification of recombinant nucleocapsid protein of Newcastle disease virus from unclarified feedstock using expanded bed adsorption chromatography
por: Tan, Yan Peng, et al.
Publicado: (2006)

Expression and purification of turkey coronavirus nucleocapsid protein in Escherichia coli
por: Loa, C.C., et al.
Publicado: (2004)

Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS
por: Timani, Khalid Amine, et al.
Publicado: (2004)

Enhancement of Solubility, Purification, and Inclusion Body Refolding of Active Human Mitochondrial Aldehyde Dehydrogenase 2
por: Zhao, Tingting, et al.
Publicado: (2021)

Performance of nucleocapsid and spike-based SARS-CoV-2 serologic assays
por: Rikhtegaran Tehrani, Zahra, et al.
Publicado: (2020)

Human post‐infection serological response to the spike and nucleocapsid proteins of SARS‐CoV‐2
por: Xiao, Cheng, et al.
Publicado: (2020)

Specificity and Performance of Nucleocapsid and Spike-based SARS-CoV-2 Serologic Assays
por: Tehrani, Zahra Rikhtegaran, et al.
Publicado: (2020)

Domain-specific biochemical and serological characterization of SARS-CoV-2 nucleocapsid protein
por: Wu, Chao, et al.
Publicado: (2021)

Thermoresponsive Gigaporous Microspheres Facilitate the Efficient Refolding of Recombinant Nitrilase Inclusion Bodies
por: Qu, Jian-Bo, et al.
Publicado: (2020)

Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli
por: Loa, Chien Chang, et al.
Publicado: (2015)

Rapid Purification of Recombinant Histones
por: Klinker, Henrike, et al.
Publicado: (2014)

Expression, purification and characterization of α-synuclein fibrillar specific scFv from inclusion bodies
por: Gupta, Vijay, et al.
Publicado: (2020)

Retraction of “Thermoresponsive Gigaporous Microspheres Facilitate the Efficient Refolding of Recombinant Nitrilase Inclusion Bodies”
por: Qu, Jian-Bo, et al.
Publicado: (2021)

Inclusion bodies as potential vehicles for recombinant protein delivery into epithelial cells
por: Liovic, Mirjana, et al.
Publicado: (2012)

Inclusion Body Expression and Refolding of Recombinant Bone Morphogenetic Protein-2
por: Nasrabadi, Davood, et al.
Publicado: (2018)

Serological detection of antibodies to the SARS-CoV-2 nucleocapsid protein from dried blood spots
por: Dhar, Arko, et al.
Publicado: (2022)

Purification of Recombinant Galectins Expressed in Bacteria
por: Prato, Cecilia Arahí, et al.
Publicado: (2020)

Evolution of infectious bronchitis virus in Taiwan: Characterisation of RNA recombination in the nucleocapsid gene
por: Kuo, Shu-Ming, et al.
Publicado: (2010)

Antibodies Produced Toward Recombinant RBD and Nucleocapsid Neutralize SARS-COV-2
por: Rezaei, Amir, et al.
Publicado: (2022)

Refolding Techniques for Recovering Biologically Active Recombinant Proteins from Inclusion Bodies
por: Yamaguchi, Hiroshi, et al.
Publicado: (2014)

Efficient recovery of recombinant CRM197 expressed as inclusion bodies in E.coli
por: Park, Ah-Reum, et al.
Publicado: (2018)

Expression and Purification along with Evaluation of Serological Response and Diagnostic Potential of Recombinant Sap2 Protein from C. parapsilosis for Use in Systemic Candidiasis
por: Shukla, Manisha, et al.
Publicado: (2021)

Data on enhanced expression and purification of camelid single domain antibodies from Escherichia coli classical inclusion bodies
por: Maggi, Maristella, et al.
Publicado: (2017)

Studies on the Inclusion Complexes of Daidzein with β-Cyclodextrin and Derivatives
por: Li, Shujing, et al.
Publicado: (2017)

Purification and kinetic studies of recombinant gibberellin dioxygenases
por: Lester, Diane R, et al.
Publicado: (2005)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Expression and Purification of Chaperone-Active Recombinant Clusterin
por: Dabbs, Rebecca A., et al.
Publicado: (2014)

Challenges and opportunities in the purification of recombinant tagged proteins
por: Pina, Ana Sofia, et al.
Publicado: (2014)

Profiling of external metabolites during production of hantavirus nucleocapsid protein with recombinant Saccharomyces cerevisiae
por: Antoniukas, Linas, et al.
Publicado: (2007)

Recombinant nucleocapsid protein is potentially an inexpensive, effective serodiagnostic reagent for infectious bronchitis virus
por: Ndifuna, Abdul, et al.
Publicado: (1998)

Cannot write session to /tmp/vufind_sessions/sess_plvmpvs3t4vn0grc77oecc37s4