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New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer

Breast cancer is one of the most prevalent forms of cancer globally and is among the leading causes of death in women. Its heterogenic nature is a result of the involvement of numerous aberrant genes that contribute to the multi-step pathway of tumorigenesis. Despite the fact that several disease-ca...

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Detalles Bibliográficos
Autores principales: Ahmed, Munazza, Daoud, Grace Hope, Mohamed, Asmaa, Harati, Rania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150278/
https://www.ncbi.nlm.nih.gov/pubmed/34066014
http://dx.doi.org/10.3390/genes12050723
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author Ahmed, Munazza
Daoud, Grace Hope
Mohamed, Asmaa
Harati, Rania
author_facet Ahmed, Munazza
Daoud, Grace Hope
Mohamed, Asmaa
Harati, Rania
author_sort Ahmed, Munazza
collection PubMed
description Breast cancer is one of the most prevalent forms of cancer globally and is among the leading causes of death in women. Its heterogenic nature is a result of the involvement of numerous aberrant genes that contribute to the multi-step pathway of tumorigenesis. Despite the fact that several disease-causing mutations have been identified, therapy is often aimed at alleviating symptoms rather than rectifying the mutation in the DNA sequence. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 is a groundbreaking tool that is being utilized for the identification and validation of genomic targets bearing tumorigenic potential. CRISPR/Cas9 supersedes its gene-editing predecessors through its unparalleled simplicity, efficiency and affordability. In this review, we provide an overview of the CRISPR/Cas9 mechanism and discuss genes that were edited using this system for the treatment of breast cancer. In addition, we shed light on the delivery methods—both viral and non-viral—that may be used to deliver the system and the barriers associated with each. Overall, the present review provides new insights into the potential therapeutic applications of CRISPR/Cas9 for the advancement of breast cancer treatment.
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spelling pubmed-81502782021-05-27 New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer Ahmed, Munazza Daoud, Grace Hope Mohamed, Asmaa Harati, Rania Genes (Basel) Review Breast cancer is one of the most prevalent forms of cancer globally and is among the leading causes of death in women. Its heterogenic nature is a result of the involvement of numerous aberrant genes that contribute to the multi-step pathway of tumorigenesis. Despite the fact that several disease-causing mutations have been identified, therapy is often aimed at alleviating symptoms rather than rectifying the mutation in the DNA sequence. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 is a groundbreaking tool that is being utilized for the identification and validation of genomic targets bearing tumorigenic potential. CRISPR/Cas9 supersedes its gene-editing predecessors through its unparalleled simplicity, efficiency and affordability. In this review, we provide an overview of the CRISPR/Cas9 mechanism and discuss genes that were edited using this system for the treatment of breast cancer. In addition, we shed light on the delivery methods—both viral and non-viral—that may be used to deliver the system and the barriers associated with each. Overall, the present review provides new insights into the potential therapeutic applications of CRISPR/Cas9 for the advancement of breast cancer treatment. MDPI 2021-05-12 /pmc/articles/PMC8150278/ /pubmed/34066014 http://dx.doi.org/10.3390/genes12050723 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ahmed, Munazza
Daoud, Grace Hope
Mohamed, Asmaa
Harati, Rania
New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer
title New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer
title_full New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer
title_fullStr New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer
title_full_unstemmed New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer
title_short New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer
title_sort new insights into the therapeutic applications of crispr/cas9 genome editing in breast cancer
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150278/
https://www.ncbi.nlm.nih.gov/pubmed/34066014
http://dx.doi.org/10.3390/genes12050723
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