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Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data?
Reticulated platelets (RP) are the youngest platelet fraction released into the circulation. These immature platelets have increased RNA content, a larger cell volume, more dense granules, higher levels of surface activation markers and are thought to be more reactive compared to their mature counte...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150321/ https://www.ncbi.nlm.nih.gov/pubmed/34065800 http://dx.doi.org/10.3390/cells10051172 |
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author | Hamad, Muataz Ali Schanze, Nancy Schommer, Nicolas Nührenberg, Thomas Duerschmied, Daniel |
author_facet | Hamad, Muataz Ali Schanze, Nancy Schommer, Nicolas Nührenberg, Thomas Duerschmied, Daniel |
author_sort | Hamad, Muataz Ali |
collection | PubMed |
description | Reticulated platelets (RP) are the youngest platelet fraction released into the circulation. These immature platelets have increased RNA content, a larger cell volume, more dense granules, higher levels of surface activation markers and are thought to be more reactive compared to their mature counterparts. RP have been associated with cardiovascular disease, diabetes and increased mortality. Yet only a few animal studies investigating RP have been conducted so far and further investigations are warranted. Established methods to count RP are flow cytometry (staining with thiazole orange or SYTO13) or fully automated hematology analyzers (immature platelet fraction, IPF). IPF has been established as a diagnostic parameter in thrombocytopenia, cardiovascular disease and, in particular, the response to antiplatelet therapy. This review seeks to provide an overview of the key features of RP as well as preanalytical and analytical aspects that need to be considered when working with this platelet population. |
format | Online Article Text |
id | pubmed-8150321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81503212021-05-27 Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? Hamad, Muataz Ali Schanze, Nancy Schommer, Nicolas Nührenberg, Thomas Duerschmied, Daniel Cells Review Reticulated platelets (RP) are the youngest platelet fraction released into the circulation. These immature platelets have increased RNA content, a larger cell volume, more dense granules, higher levels of surface activation markers and are thought to be more reactive compared to their mature counterparts. RP have been associated with cardiovascular disease, diabetes and increased mortality. Yet only a few animal studies investigating RP have been conducted so far and further investigations are warranted. Established methods to count RP are flow cytometry (staining with thiazole orange or SYTO13) or fully automated hematology analyzers (immature platelet fraction, IPF). IPF has been established as a diagnostic parameter in thrombocytopenia, cardiovascular disease and, in particular, the response to antiplatelet therapy. This review seeks to provide an overview of the key features of RP as well as preanalytical and analytical aspects that need to be considered when working with this platelet population. MDPI 2021-05-12 /pmc/articles/PMC8150321/ /pubmed/34065800 http://dx.doi.org/10.3390/cells10051172 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Hamad, Muataz Ali Schanze, Nancy Schommer, Nicolas Nührenberg, Thomas Duerschmied, Daniel Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? |
title | Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? |
title_full | Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? |
title_fullStr | Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? |
title_full_unstemmed | Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? |
title_short | Reticulated Platelets—Which Functions Have Been Established by In Vivo and In Vitro Data? |
title_sort | reticulated platelets—which functions have been established by in vivo and in vitro data? |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150321/ https://www.ncbi.nlm.nih.gov/pubmed/34065800 http://dx.doi.org/10.3390/cells10051172 |
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