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RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii
A homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in Acinetobacter baumannii. Here, we evaluated the industrial version of this test, the RapidR...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150362/ https://www.ncbi.nlm.nih.gov/pubmed/34064682 http://dx.doi.org/10.3390/antibiotics10050558 |
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author | Bouvier, Maxime Sadek, Mustafa Pomponio, Stefano D’Emidio, Fernando Poirel, Laurent Nordmann, Patrice |
author_facet | Bouvier, Maxime Sadek, Mustafa Pomponio, Stefano D’Emidio, Fernando Poirel, Laurent Nordmann, Patrice |
author_sort | Bouvier, Maxime |
collection | PubMed |
description | A homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in Acinetobacter baumannii. Here, we evaluated the industrial version of this test, the RapidResa Polymyxin Acinetobacter NP(®) test (Liofilchem, Italy). A well-characterized panel of 68 clinical A. baumannii strains (36 polymyxin-susceptible, 26 polymyxin-resistant A. baumannii, and 6 colistin-heteroresistant isolates) of worldwide origin was tested. All the colistin-susceptible A. baumannii isolates gave negative results according to the RapidResa Polymyxin Acinetobacter NP(®) test, except for a single isolate that gave a false-positive result. Out of the 26 colistin-resistant A. baumannii strains, 25 were correctly identified as colistin resistant using the RapidResa Polymyxin Acinetobacter NP(®) test. Only a single colistin-resistant A. baumannii strain gave a false-negative result. Additionally, the six colistin-heteroresistant isolates tested gave positive results. Altogether, the sensitivity and the specificity of the test were found to be 96% and 97%, respectively. The turn-around-time of this easy-to-perform test was 3-4h, which showed excellent reliability for identification of polymyxin resistance in A. baumannii. The RapidResa Polymyxin Acinetobacter NP(®) test allows a rapid differentiation between polymyxin-susceptible and -resistant A. baumannii isolates, which may contribute to optimization of the use of polymyxins for treating infections due to multidrug-resistant A. baumannii. |
format | Online Article Text |
id | pubmed-8150362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81503622021-05-27 RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii Bouvier, Maxime Sadek, Mustafa Pomponio, Stefano D’Emidio, Fernando Poirel, Laurent Nordmann, Patrice Antibiotics (Basel) Brief Report A homemade and culture-based test, relying on the visual detection of the reduction of the resazurin reagent (a cell viability indicator), has been developed for the rapid detection of polymyxin resistance in Acinetobacter baumannii. Here, we evaluated the industrial version of this test, the RapidResa Polymyxin Acinetobacter NP(®) test (Liofilchem, Italy). A well-characterized panel of 68 clinical A. baumannii strains (36 polymyxin-susceptible, 26 polymyxin-resistant A. baumannii, and 6 colistin-heteroresistant isolates) of worldwide origin was tested. All the colistin-susceptible A. baumannii isolates gave negative results according to the RapidResa Polymyxin Acinetobacter NP(®) test, except for a single isolate that gave a false-positive result. Out of the 26 colistin-resistant A. baumannii strains, 25 were correctly identified as colistin resistant using the RapidResa Polymyxin Acinetobacter NP(®) test. Only a single colistin-resistant A. baumannii strain gave a false-negative result. Additionally, the six colistin-heteroresistant isolates tested gave positive results. Altogether, the sensitivity and the specificity of the test were found to be 96% and 97%, respectively. The turn-around-time of this easy-to-perform test was 3-4h, which showed excellent reliability for identification of polymyxin resistance in A. baumannii. The RapidResa Polymyxin Acinetobacter NP(®) test allows a rapid differentiation between polymyxin-susceptible and -resistant A. baumannii isolates, which may contribute to optimization of the use of polymyxins for treating infections due to multidrug-resistant A. baumannii. MDPI 2021-05-11 /pmc/articles/PMC8150362/ /pubmed/34064682 http://dx.doi.org/10.3390/antibiotics10050558 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Bouvier, Maxime Sadek, Mustafa Pomponio, Stefano D’Emidio, Fernando Poirel, Laurent Nordmann, Patrice RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii |
title | RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii |
title_full | RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii |
title_fullStr | RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii |
title_full_unstemmed | RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii |
title_short | RapidResa Polymyxin Acinetobacter NP(®) Test for Rapid Detection of Polymyxin Resistance in Acinetobacter baumannii |
title_sort | rapidresa polymyxin acinetobacter np(®) test for rapid detection of polymyxin resistance in acinetobacter baumannii |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150362/ https://www.ncbi.nlm.nih.gov/pubmed/34064682 http://dx.doi.org/10.3390/antibiotics10050558 |
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