Cargando…

Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures

Recently, the oxidative behavior of methotrexate (MTX) anticancer drug is highly demanded, due to its side effects on healthy cells, despite being a very challenging task. In this study, we have prepared porous NiO material using sodium sulfate as an electronic disorder reagent by hydrothermal metho...

Descripción completa

Detalles Bibliográficos
Autores principales: Khand, Aftab A., Lakho, Saeed A., Tahira, Aneela, Ubaidullah, Mohd, Alothman, Asma A., Aljadoa, Khoulwod, Nafady, Ayman, Ibupoto, Zafar H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150394/
https://www.ncbi.nlm.nih.gov/pubmed/34065856
http://dx.doi.org/10.3390/nano11051266
_version_ 1783698141651927040
author Khand, Aftab A.
Lakho, Saeed A.
Tahira, Aneela
Ubaidullah, Mohd
Alothman, Asma A.
Aljadoa, Khoulwod
Nafady, Ayman
Ibupoto, Zafar H.
author_facet Khand, Aftab A.
Lakho, Saeed A.
Tahira, Aneela
Ubaidullah, Mohd
Alothman, Asma A.
Aljadoa, Khoulwod
Nafady, Ayman
Ibupoto, Zafar H.
author_sort Khand, Aftab A.
collection PubMed
description Recently, the oxidative behavior of methotrexate (MTX) anticancer drug is highly demanded, due to its side effects on healthy cells, despite being a very challenging task. In this study, we have prepared porous NiO material using sodium sulfate as an electronic disorder reagent by hydrothermal method and found it highly sensitive and selective for the oxidation of MTX. The synthesized NiO nanostructures were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. These physical characterizations delineated the porous morphology and cubic crystalline phase of NiO. Different electrochemical approaches have been utilized to determine the MTX concentrations in 0.04 M Britton–Robinson buffer (BRB) at pH 2 using glassy carbon electrode (GCE)-modified with electronically disordered NiO nanostructures. The linear range for MTX using cyclic voltammetry (CV) was found to be from 5 to 30 nM, and the limit of detection (LOD) and limit of quantification (LOQ) were 1.46 nM and 4.86 nM, respectively, whereas the linear range obtained via linear sweep voltammetry (LSV) was estimated as 15–90 nM with LOD and LOQ of 0.819 nM and 2.713 nM, respectively. Additionally, amperometric studies revealed a linear range from 10 to70 nM with LOD and LOQ of 0.1 nM and 1.3 nM, respectively. Importantly, MTX was successfully monitored in pharmaceutical products using the standard recovery method. Thus, the proposed approach for the synthesis of active metal oxide materials could be sued for the determination of other anticancer drugs in real samples and other biomedical applications.
format Online
Article
Text
id pubmed-8150394
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81503942021-05-27 Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures Khand, Aftab A. Lakho, Saeed A. Tahira, Aneela Ubaidullah, Mohd Alothman, Asma A. Aljadoa, Khoulwod Nafady, Ayman Ibupoto, Zafar H. Nanomaterials (Basel) Article Recently, the oxidative behavior of methotrexate (MTX) anticancer drug is highly demanded, due to its side effects on healthy cells, despite being a very challenging task. In this study, we have prepared porous NiO material using sodium sulfate as an electronic disorder reagent by hydrothermal method and found it highly sensitive and selective for the oxidation of MTX. The synthesized NiO nanostructures were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. These physical characterizations delineated the porous morphology and cubic crystalline phase of NiO. Different electrochemical approaches have been utilized to determine the MTX concentrations in 0.04 M Britton–Robinson buffer (BRB) at pH 2 using glassy carbon electrode (GCE)-modified with electronically disordered NiO nanostructures. The linear range for MTX using cyclic voltammetry (CV) was found to be from 5 to 30 nM, and the limit of detection (LOD) and limit of quantification (LOQ) were 1.46 nM and 4.86 nM, respectively, whereas the linear range obtained via linear sweep voltammetry (LSV) was estimated as 15–90 nM with LOD and LOQ of 0.819 nM and 2.713 nM, respectively. Additionally, amperometric studies revealed a linear range from 10 to70 nM with LOD and LOQ of 0.1 nM and 1.3 nM, respectively. Importantly, MTX was successfully monitored in pharmaceutical products using the standard recovery method. Thus, the proposed approach for the synthesis of active metal oxide materials could be sued for the determination of other anticancer drugs in real samples and other biomedical applications. MDPI 2021-05-12 /pmc/articles/PMC8150394/ /pubmed/34065856 http://dx.doi.org/10.3390/nano11051266 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khand, Aftab A.
Lakho, Saeed A.
Tahira, Aneela
Ubaidullah, Mohd
Alothman, Asma A.
Aljadoa, Khoulwod
Nafady, Ayman
Ibupoto, Zafar H.
Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
title Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
title_full Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
title_fullStr Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
title_full_unstemmed Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
title_short Facile Electrochemical Determination of Methotrexate (MTX) Using Glassy Carbon Electrode-Modified with Electronically Disordered NiO Nanostructures
title_sort facile electrochemical determination of methotrexate (mtx) using glassy carbon electrode-modified with electronically disordered nio nanostructures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150394/
https://www.ncbi.nlm.nih.gov/pubmed/34065856
http://dx.doi.org/10.3390/nano11051266
work_keys_str_mv AT khandaftaba facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT lakhosaeeda facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT tahiraaneela facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT ubaidullahmohd facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT alothmanasmaa facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT aljadoakhoulwod facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT nafadyayman facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures
AT ibupotozafarh facileelectrochemicaldeterminationofmethotrexatemtxusingglassycarbonelectrodemodifiedwithelectronicallydisorderednionanostructures