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Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy
Rapid identification and characterization of multidrug-resistant Klebsiella pneumoniae strains is essential to diagnose severe infections in patients. In clinical routine practice, K. pneumoniae is frequently identified and characterized for outbreak investigation. Pulsed-field gel electrophoresis o...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150785/ https://www.ncbi.nlm.nih.gov/pubmed/34064924 http://dx.doi.org/10.3390/antibiotics10050560 |
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author | Fasciana, Teresa Ciammaruconi, Andrea Gentile, Bernardina Di Carlo, Paola Virruso, Roberta Tricoli, Maria Rita Palma, Daniela Maria Pitarresi, Giovanna Laura Lista, Florigio Giammanco, Anna |
author_facet | Fasciana, Teresa Ciammaruconi, Andrea Gentile, Bernardina Di Carlo, Paola Virruso, Roberta Tricoli, Maria Rita Palma, Daniela Maria Pitarresi, Giovanna Laura Lista, Florigio Giammanco, Anna |
author_sort | Fasciana, Teresa |
collection | PubMed |
description | Rapid identification and characterization of multidrug-resistant Klebsiella pneumoniae strains is essential to diagnose severe infections in patients. In clinical routine practice, K. pneumoniae is frequently identified and characterized for outbreak investigation. Pulsed-field gel electrophoresis or multilocus sequence typing could be used, but, unfortunately, these methods are time-consuming, laborious, expensive, and do not provide any information about the presence of resistance and virulence genes. In recent years, the decreasing cost of next-generation sequencing and its easy use have led to it being considered a useful method, not only for outbreak surveillance but also for rapid identification and evaluation, in a single step, of virulence factors and resistance genes. Carbapenem-resistant strains of K. pneumoniae have become endemic in Italy, and in these strains the ability to form biofilms, communities of bacteria fixed in an extracellular matrix, can defend the pathogen from the host immune response as well as from antibiotics, improving its persistence in epithelial tissues and on medical device surfaces. |
format | Online Article Text |
id | pubmed-8150785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81507852021-05-27 Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy Fasciana, Teresa Ciammaruconi, Andrea Gentile, Bernardina Di Carlo, Paola Virruso, Roberta Tricoli, Maria Rita Palma, Daniela Maria Pitarresi, Giovanna Laura Lista, Florigio Giammanco, Anna Antibiotics (Basel) Communication Rapid identification and characterization of multidrug-resistant Klebsiella pneumoniae strains is essential to diagnose severe infections in patients. In clinical routine practice, K. pneumoniae is frequently identified and characterized for outbreak investigation. Pulsed-field gel electrophoresis or multilocus sequence typing could be used, but, unfortunately, these methods are time-consuming, laborious, expensive, and do not provide any information about the presence of resistance and virulence genes. In recent years, the decreasing cost of next-generation sequencing and its easy use have led to it being considered a useful method, not only for outbreak surveillance but also for rapid identification and evaluation, in a single step, of virulence factors and resistance genes. Carbapenem-resistant strains of K. pneumoniae have become endemic in Italy, and in these strains the ability to form biofilms, communities of bacteria fixed in an extracellular matrix, can defend the pathogen from the host immune response as well as from antibiotics, improving its persistence in epithelial tissues and on medical device surfaces. MDPI 2021-05-11 /pmc/articles/PMC8150785/ /pubmed/34064924 http://dx.doi.org/10.3390/antibiotics10050560 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Fasciana, Teresa Ciammaruconi, Andrea Gentile, Bernardina Di Carlo, Paola Virruso, Roberta Tricoli, Maria Rita Palma, Daniela Maria Pitarresi, Giovanna Laura Lista, Florigio Giammanco, Anna Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy |
title | Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy |
title_full | Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy |
title_fullStr | Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy |
title_full_unstemmed | Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy |
title_short | Draft Genome Sequence and Biofilm Production of a Carbapenemase-Producing Klebsiella pneumoniae (KpR405) Sequence Type 405 Strain Isolated in Italy |
title_sort | draft genome sequence and biofilm production of a carbapenemase-producing klebsiella pneumoniae (kpr405) sequence type 405 strain isolated in italy |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150785/ https://www.ncbi.nlm.nih.gov/pubmed/34064924 http://dx.doi.org/10.3390/antibiotics10050560 |
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