Cargando…
Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity
This study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate th...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150829/ https://www.ncbi.nlm.nih.gov/pubmed/34064830 http://dx.doi.org/10.3390/antiox10050761 |
_version_ | 1783698240892305408 |
---|---|
author | Yeo, Dahee Hwang, Su-Jung Song, Ye-Seul Lee, Hyo-Jong |
author_facet | Yeo, Dahee Hwang, Su-Jung Song, Ye-Seul Lee, Hyo-Jong |
author_sort | Yeo, Dahee |
collection | PubMed |
description | This study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate the mucosal protective effect of α-humulene. α-Humulene significantly inhibited gastric lesions in HCl/ethanol-induced acute gastritis and decreased gastric acid secretion pyloric ligation-induced gastric ulcers in vivo. In addition, α-humulene reduced the amount of reactive oxygen species and malondialdehyde through upregulation of prostaglandin E2 (PGE2) and superoxide dismutase (SOD). In HMC-1 cells, α-humulene decreased intracellular calcium and increased intracellular cyclic adenosine monophosphate (cAMP) levels, resulting in low histamine levels. α-Humulene also reduced the expression levels of cytokine genes such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF) by downregulating nuclear factor-κB (NF-κB) nuclear translocation. Finally, α-humulene upregulated the expression levels of mucin 5AC (Muc5ac), Muc6, trefoil factor 1 (Tff1), trefoil factor 2 (Tff2), and polymeric immunoglobulin receptor (pigr). α-Humulene may attenuate HCl/ethanol-induced gastritis by inhibiting histamine release and NF-κB activation and stimulating antioxidants and mucosal protective factors, particularly Muc5ac and Muc6. Therefore, these data suggest that α-humulene is a potential drug candidate for the treatment of stress-induced or alcoholic gastritis. |
format | Online Article Text |
id | pubmed-8150829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81508292021-05-27 Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity Yeo, Dahee Hwang, Su-Jung Song, Ye-Seul Lee, Hyo-Jong Antioxidants (Basel) Article This study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate the mucosal protective effect of α-humulene. α-Humulene significantly inhibited gastric lesions in HCl/ethanol-induced acute gastritis and decreased gastric acid secretion pyloric ligation-induced gastric ulcers in vivo. In addition, α-humulene reduced the amount of reactive oxygen species and malondialdehyde through upregulation of prostaglandin E2 (PGE2) and superoxide dismutase (SOD). In HMC-1 cells, α-humulene decreased intracellular calcium and increased intracellular cyclic adenosine monophosphate (cAMP) levels, resulting in low histamine levels. α-Humulene also reduced the expression levels of cytokine genes such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF) by downregulating nuclear factor-κB (NF-κB) nuclear translocation. Finally, α-humulene upregulated the expression levels of mucin 5AC (Muc5ac), Muc6, trefoil factor 1 (Tff1), trefoil factor 2 (Tff2), and polymeric immunoglobulin receptor (pigr). α-Humulene may attenuate HCl/ethanol-induced gastritis by inhibiting histamine release and NF-κB activation and stimulating antioxidants and mucosal protective factors, particularly Muc5ac and Muc6. Therefore, these data suggest that α-humulene is a potential drug candidate for the treatment of stress-induced or alcoholic gastritis. MDPI 2021-05-11 /pmc/articles/PMC8150829/ /pubmed/34064830 http://dx.doi.org/10.3390/antiox10050761 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yeo, Dahee Hwang, Su-Jung Song, Ye-Seul Lee, Hyo-Jong Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
title | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
title_full | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
title_fullStr | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
title_full_unstemmed | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
title_short | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
title_sort | humulene inhibits acute gastric mucosal injury by enhancing mucosal integrity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150829/ https://www.ncbi.nlm.nih.gov/pubmed/34064830 http://dx.doi.org/10.3390/antiox10050761 |
work_keys_str_mv | AT yeodahee humuleneinhibitsacutegastricmucosalinjurybyenhancingmucosalintegrity AT hwangsujung humuleneinhibitsacutegastricmucosalinjurybyenhancingmucosalintegrity AT songyeseul humuleneinhibitsacutegastricmucosalinjurybyenhancingmucosalintegrity AT leehyojong humuleneinhibitsacutegastricmucosalinjurybyenhancingmucosalintegrity |