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Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo

Background: Angiogenesis is well known to be an important event in the tissue remodeling observed in allergic diseases. Although there is much evidence that quercetin, one of the most abundant dietary flavonoids, exerts anti-allergic effects in both human and experimental animal models of allergic d...

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Autores principales: Okumo, Takayuki, Furuta, Atsuko, Kimura, Tarou, Yusa, Kanako, Asano, Kazuhito, Sunagawa, Masataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150841/
https://www.ncbi.nlm.nih.gov/pubmed/34065895
http://dx.doi.org/10.3390/medicines8050022
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author Okumo, Takayuki
Furuta, Atsuko
Kimura, Tarou
Yusa, Kanako
Asano, Kazuhito
Sunagawa, Masataka
author_facet Okumo, Takayuki
Furuta, Atsuko
Kimura, Tarou
Yusa, Kanako
Asano, Kazuhito
Sunagawa, Masataka
author_sort Okumo, Takayuki
collection PubMed
description Background: Angiogenesis is well known to be an important event in the tissue remodeling observed in allergic diseases. Although there is much evidence that quercetin, one of the most abundant dietary flavonoids, exerts anti-allergic effects in both human and experimental animal models of allergic diseases, the action of quercetin on angiogenesis has not been defined. Therefore, in this study, we first examined the action of quercetin on the secretion of angiogenic factors from murine mast cells in vitro. We also examined the action of quercetin on angiogenic factor secretion in the murine allergic rhinitis model in vivo. Methods: Mast cells (1 × 105 cells/mL) sensitized with ovalbumin (OVA)-specific murine IgE were stimulated with 10.0 ng/mL OVA in the presence or the absence of quercetin for 24 h. The concentrations of angiogenic factors, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α, IL-6 and IL-8 in the supernatants were examined by ELISA. BALB/c male mice immunized with OVA were challenged intranasally with OVA every other day, starting seven days after the final immunization. These mice were then orally administered quercetin once a day for five days, starting seven days after the final immunization. Clinical symptoms were assessed by counting the number of sneezes and nasal rubbing behaviors during the 10 min period just after OVA nasal provocation. The angiogenic factor concentrations in the nasal lavage fluids obtained 6 h after nasal antigenic provocation were examined by ELISA. Results: Quercetin significantly inhibited the production of angiogenetic factors induced by IgE-dependent mechanisms at 5.0 µM or more. Oral administration of 25.0 mg/kg quercetin into the mice also suppressed the appearance of angiogenetic factors in nasal lavage fluids, along with the attenuation of nasal symptoms. Conclusions: These results strongly suggest that the inhibitory action of quercetin on angiogenic factor secretion may be implicated in the therapeutic action of quercetin on allergic diseases, especially allergic rhinitis.
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spelling pubmed-81508412021-05-27 Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo Okumo, Takayuki Furuta, Atsuko Kimura, Tarou Yusa, Kanako Asano, Kazuhito Sunagawa, Masataka Medicines (Basel) Article Background: Angiogenesis is well known to be an important event in the tissue remodeling observed in allergic diseases. Although there is much evidence that quercetin, one of the most abundant dietary flavonoids, exerts anti-allergic effects in both human and experimental animal models of allergic diseases, the action of quercetin on angiogenesis has not been defined. Therefore, in this study, we first examined the action of quercetin on the secretion of angiogenic factors from murine mast cells in vitro. We also examined the action of quercetin on angiogenic factor secretion in the murine allergic rhinitis model in vivo. Methods: Mast cells (1 × 105 cells/mL) sensitized with ovalbumin (OVA)-specific murine IgE were stimulated with 10.0 ng/mL OVA in the presence or the absence of quercetin for 24 h. The concentrations of angiogenic factors, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), tumor necrosis factor-α, IL-6 and IL-8 in the supernatants were examined by ELISA. BALB/c male mice immunized with OVA were challenged intranasally with OVA every other day, starting seven days after the final immunization. These mice were then orally administered quercetin once a day for five days, starting seven days after the final immunization. Clinical symptoms were assessed by counting the number of sneezes and nasal rubbing behaviors during the 10 min period just after OVA nasal provocation. The angiogenic factor concentrations in the nasal lavage fluids obtained 6 h after nasal antigenic provocation were examined by ELISA. Results: Quercetin significantly inhibited the production of angiogenetic factors induced by IgE-dependent mechanisms at 5.0 µM or more. Oral administration of 25.0 mg/kg quercetin into the mice also suppressed the appearance of angiogenetic factors in nasal lavage fluids, along with the attenuation of nasal symptoms. Conclusions: These results strongly suggest that the inhibitory action of quercetin on angiogenic factor secretion may be implicated in the therapeutic action of quercetin on allergic diseases, especially allergic rhinitis. MDPI 2021-05-12 /pmc/articles/PMC8150841/ /pubmed/34065895 http://dx.doi.org/10.3390/medicines8050022 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Okumo, Takayuki
Furuta, Atsuko
Kimura, Tarou
Yusa, Kanako
Asano, Kazuhito
Sunagawa, Masataka
Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo
title Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo
title_full Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo
title_fullStr Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo
title_full_unstemmed Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo
title_short Inhibition of Angiogenic Factor Productions by Quercetin In Vitro and In Vivo
title_sort inhibition of angiogenic factor productions by quercetin in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150841/
https://www.ncbi.nlm.nih.gov/pubmed/34065895
http://dx.doi.org/10.3390/medicines8050022
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