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Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol
The study evaluated the chlorogenic acid (CGA) antioxidant potential on oxidative stress (OS) induced in vitro in human spermatozoa and during cryopreservation procedure. Swim-up selected spermatozoa were treated with 100 µM CGA, 100 µM H(2)O(2) to induce lipid peroxidation (LPO), and with both comp...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150895/ https://www.ncbi.nlm.nih.gov/pubmed/34067222 http://dx.doi.org/10.3390/antiox10050744 |
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author | Noto, Daria Collodel, Giulia Cerretani, Daniela Signorini, Cinzia Gambera, Laura Menchiari, Andrea Moretti, Elena |
author_facet | Noto, Daria Collodel, Giulia Cerretani, Daniela Signorini, Cinzia Gambera, Laura Menchiari, Andrea Moretti, Elena |
author_sort | Noto, Daria |
collection | PubMed |
description | The study evaluated the chlorogenic acid (CGA) antioxidant potential on oxidative stress (OS) induced in vitro in human spermatozoa and during cryopreservation procedure. Swim-up selected spermatozoa were treated with 100 µM CGA, 100 µM H(2)O(2) to induce lipid peroxidation (LPO), and with both compounds and the effects on mitochondrial membrane potential (MMP) by JC-1, DNA integrity by acridine orange (AO), and sperm ultrastructure by transmission electron microscopy (TEM), were evaluated. CGA antioxidant activity was assessed by measuring malondialdehyde (MDA) and F(2)-isoprostanes (F(2)-IsoPs) in the media. The CGA protective activity and the immunolocalization of Phospho-AMPKα (Thr172) were explored in frozen-thawed sperm. CGA was not toxic for sperm motility, DNA integrity and MMP. The increase in MDA (p < 0.05) and F(2)-IsoPs (p < 0.001), DNA damage (p < 0.01) and low MMP (p < 0.01) levels after H(2)O(2) treatment were reduced in presence of CGA as well as the percentage of broken plasma membranes (p < 0.01) and altered acrosomes (p < 0.01) detected by TEM. Treated frozen-thawed spermatozoa showed increased sperm motility (p < 0.01), DNA integrity (p < 0.01), MMP (p < 0.01), reduced MDA (p < 0.01) and increased sperm percentage with Phospho-AMPKα labelling in the head (p < 0.001). CGA can be used to supplement culture media during semen handling and cryopreservation where OS is exacerbated. |
format | Online Article Text |
id | pubmed-8150895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81508952021-05-27 Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol Noto, Daria Collodel, Giulia Cerretani, Daniela Signorini, Cinzia Gambera, Laura Menchiari, Andrea Moretti, Elena Antioxidants (Basel) Article The study evaluated the chlorogenic acid (CGA) antioxidant potential on oxidative stress (OS) induced in vitro in human spermatozoa and during cryopreservation procedure. Swim-up selected spermatozoa were treated with 100 µM CGA, 100 µM H(2)O(2) to induce lipid peroxidation (LPO), and with both compounds and the effects on mitochondrial membrane potential (MMP) by JC-1, DNA integrity by acridine orange (AO), and sperm ultrastructure by transmission electron microscopy (TEM), were evaluated. CGA antioxidant activity was assessed by measuring malondialdehyde (MDA) and F(2)-isoprostanes (F(2)-IsoPs) in the media. The CGA protective activity and the immunolocalization of Phospho-AMPKα (Thr172) were explored in frozen-thawed sperm. CGA was not toxic for sperm motility, DNA integrity and MMP. The increase in MDA (p < 0.05) and F(2)-IsoPs (p < 0.001), DNA damage (p < 0.01) and low MMP (p < 0.01) levels after H(2)O(2) treatment were reduced in presence of CGA as well as the percentage of broken plasma membranes (p < 0.01) and altered acrosomes (p < 0.01) detected by TEM. Treated frozen-thawed spermatozoa showed increased sperm motility (p < 0.01), DNA integrity (p < 0.01), MMP (p < 0.01), reduced MDA (p < 0.01) and increased sperm percentage with Phospho-AMPKα labelling in the head (p < 0.001). CGA can be used to supplement culture media during semen handling and cryopreservation where OS is exacerbated. MDPI 2021-05-07 /pmc/articles/PMC8150895/ /pubmed/34067222 http://dx.doi.org/10.3390/antiox10050744 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Noto, Daria Collodel, Giulia Cerretani, Daniela Signorini, Cinzia Gambera, Laura Menchiari, Andrea Moretti, Elena Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol |
title | Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol |
title_full | Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol |
title_fullStr | Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol |
title_full_unstemmed | Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol |
title_short | Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen–Thawed Protocol |
title_sort | protective effect of chlorogenic acid on human sperm: in vitro studies and frozen–thawed protocol |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150895/ https://www.ncbi.nlm.nih.gov/pubmed/34067222 http://dx.doi.org/10.3390/antiox10050744 |
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