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Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing

This study describes a case of SARS-CoV-2 reinfection confirmed by whole-genome sequencing in a healthy physician who had been working in a COVID-19 hospital in Italy since the beginning of the pandemic. Nasopharyngeal swabs were obtained from the patient at each presentation as part of routine surv...

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Autores principales: Loconsole, Daniela, Sallustio, Anna, Accogli, Marisa, Centrone, Francesca, Casulli, Daniele, Madaro, Antonino, Tedeschi, Ersilia, Parisi, Antonio, Chironna, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150928/
https://www.ncbi.nlm.nih.gov/pubmed/34066205
http://dx.doi.org/10.3390/v13050899
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author Loconsole, Daniela
Sallustio, Anna
Accogli, Marisa
Centrone, Francesca
Casulli, Daniele
Madaro, Antonino
Tedeschi, Ersilia
Parisi, Antonio
Chironna, Maria
author_facet Loconsole, Daniela
Sallustio, Anna
Accogli, Marisa
Centrone, Francesca
Casulli, Daniele
Madaro, Antonino
Tedeschi, Ersilia
Parisi, Antonio
Chironna, Maria
author_sort Loconsole, Daniela
collection PubMed
description This study describes a case of SARS-CoV-2 reinfection confirmed by whole-genome sequencing in a healthy physician who had been working in a COVID-19 hospital in Italy since the beginning of the pandemic. Nasopharyngeal swabs were obtained from the patient at each presentation as part of routine surveillance. Nucleic acid amplification testing was performed on the two samples to confirm SARS-CoV-2 infection, and serological tests were used to detect SARS-CoV-2 IgG antibodies. Comparative genome analysis with whole-genome sequencing was performed on nasopharyngeal swabs collected during the two episodes of COVID-19. The first COVID-19 episode was in March 2020, and the second was in January 2021. Both SARS-CoV-2 infections presented with mild symptoms, and seroconversion for SARS-CoV-2 IgG was documented. Genomic analysis showed that the viral genome from the first infection belonged to the lineage B.1.1.74, while that from the second infection to the lineage B.1.177. Epidemiological, clinical, serological, and genomic analyses confirmed that the second episode of SARS-CoV-2 infection in the healthcare worker met the qualifications for “best evidence” for reinfection. Further studies are urgently needed to assess the frequency of such a worrisome occurrence, particularly in the light of the recent diffusion of SARS-CoV-2 variants of concern.
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spelling pubmed-81509282021-05-27 Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing Loconsole, Daniela Sallustio, Anna Accogli, Marisa Centrone, Francesca Casulli, Daniele Madaro, Antonino Tedeschi, Ersilia Parisi, Antonio Chironna, Maria Viruses Article This study describes a case of SARS-CoV-2 reinfection confirmed by whole-genome sequencing in a healthy physician who had been working in a COVID-19 hospital in Italy since the beginning of the pandemic. Nasopharyngeal swabs were obtained from the patient at each presentation as part of routine surveillance. Nucleic acid amplification testing was performed on the two samples to confirm SARS-CoV-2 infection, and serological tests were used to detect SARS-CoV-2 IgG antibodies. Comparative genome analysis with whole-genome sequencing was performed on nasopharyngeal swabs collected during the two episodes of COVID-19. The first COVID-19 episode was in March 2020, and the second was in January 2021. Both SARS-CoV-2 infections presented with mild symptoms, and seroconversion for SARS-CoV-2 IgG was documented. Genomic analysis showed that the viral genome from the first infection belonged to the lineage B.1.1.74, while that from the second infection to the lineage B.1.177. Epidemiological, clinical, serological, and genomic analyses confirmed that the second episode of SARS-CoV-2 infection in the healthcare worker met the qualifications for “best evidence” for reinfection. Further studies are urgently needed to assess the frequency of such a worrisome occurrence, particularly in the light of the recent diffusion of SARS-CoV-2 variants of concern. MDPI 2021-05-12 /pmc/articles/PMC8150928/ /pubmed/34066205 http://dx.doi.org/10.3390/v13050899 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loconsole, Daniela
Sallustio, Anna
Accogli, Marisa
Centrone, Francesca
Casulli, Daniele
Madaro, Antonino
Tedeschi, Ersilia
Parisi, Antonio
Chironna, Maria
Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing
title Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing
title_full Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing
title_fullStr Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing
title_full_unstemmed Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing
title_short Symptomatic SARS-CoV-2 Reinfection in a Healthy Healthcare Worker in Italy Confirmed by Whole-Genome Sequencing
title_sort symptomatic sars-cov-2 reinfection in a healthy healthcare worker in italy confirmed by whole-genome sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150928/
https://www.ncbi.nlm.nih.gov/pubmed/34066205
http://dx.doi.org/10.3390/v13050899
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