Cargando…
Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus
Field surveys were conducted in Greek olive orchards from 2017 to 2020 to collect information on the sanitary status of the trees. Using a high-throughput sequencing approach, viral sequences were identified in total RNA extracts from several trees and assembled to reconstruct the complete genomes o...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150953/ https://www.ncbi.nlm.nih.gov/pubmed/34066889 http://dx.doi.org/10.3390/pathogens10050574 |
_version_ | 1783698269085368320 |
---|---|
author | Xylogianni, Evanthia Margaria, Paolo Knierim, Dennis Sareli, Kyriaki Winter, Stephan Chatzivassiliou, Elisavet K. |
author_facet | Xylogianni, Evanthia Margaria, Paolo Knierim, Dennis Sareli, Kyriaki Winter, Stephan Chatzivassiliou, Elisavet K. |
author_sort | Xylogianni, Evanthia |
collection | PubMed |
description | Field surveys were conducted in Greek olive orchards from 2017 to 2020 to collect information on the sanitary status of the trees. Using a high-throughput sequencing approach, viral sequences were identified in total RNA extracts from several trees and assembled to reconstruct the complete genomes of two isolates of a new viral species of the genus Tepovirus (Betaflexiviridae), for which the name olive virus T (OlVT) is proposed. A reverse transcription–polymerase chain reaction assay was developed which detected OlVT in samples collected in olive growing regions in Central and Northern Greece, showing a virus prevalence of 4.4% in the olive trees screened. Sequences of amplified fragments from the movement–coat protein region of OlVT isolates varied from 75.64% to 99.35%. Three olive varieties (Koroneiki, Arbequina and Frantoio) were infected with OlVT via grafting to confirm a graft-transmissible agent, but virus infections remained latent. In addition, cucumber mosaic virus, olive leaf yellowing-associated virus and cherry leaf roll virus were identified. |
format | Online Article Text |
id | pubmed-8150953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81509532021-05-27 Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus Xylogianni, Evanthia Margaria, Paolo Knierim, Dennis Sareli, Kyriaki Winter, Stephan Chatzivassiliou, Elisavet K. Pathogens Article Field surveys were conducted in Greek olive orchards from 2017 to 2020 to collect information on the sanitary status of the trees. Using a high-throughput sequencing approach, viral sequences were identified in total RNA extracts from several trees and assembled to reconstruct the complete genomes of two isolates of a new viral species of the genus Tepovirus (Betaflexiviridae), for which the name olive virus T (OlVT) is proposed. A reverse transcription–polymerase chain reaction assay was developed which detected OlVT in samples collected in olive growing regions in Central and Northern Greece, showing a virus prevalence of 4.4% in the olive trees screened. Sequences of amplified fragments from the movement–coat protein region of OlVT isolates varied from 75.64% to 99.35%. Three olive varieties (Koroneiki, Arbequina and Frantoio) were infected with OlVT via grafting to confirm a graft-transmissible agent, but virus infections remained latent. In addition, cucumber mosaic virus, olive leaf yellowing-associated virus and cherry leaf roll virus were identified. MDPI 2021-05-08 /pmc/articles/PMC8150953/ /pubmed/34066889 http://dx.doi.org/10.3390/pathogens10050574 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xylogianni, Evanthia Margaria, Paolo Knierim, Dennis Sareli, Kyriaki Winter, Stephan Chatzivassiliou, Elisavet K. Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus |
title | Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus |
title_full | Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus |
title_fullStr | Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus |
title_full_unstemmed | Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus |
title_short | Virus Surveys in Olive Orchards in Greece Identify Olive Virus T, a Novel Member of the Genus Tepovirus |
title_sort | virus surveys in olive orchards in greece identify olive virus t, a novel member of the genus tepovirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8150953/ https://www.ncbi.nlm.nih.gov/pubmed/34066889 http://dx.doi.org/10.3390/pathogens10050574 |
work_keys_str_mv | AT xylogiannievanthia virussurveysinoliveorchardsingreeceidentifyolivevirustanovelmemberofthegenustepovirus AT margariapaolo virussurveysinoliveorchardsingreeceidentifyolivevirustanovelmemberofthegenustepovirus AT knierimdennis virussurveysinoliveorchardsingreeceidentifyolivevirustanovelmemberofthegenustepovirus AT sarelikyriaki virussurveysinoliveorchardsingreeceidentifyolivevirustanovelmemberofthegenustepovirus AT winterstephan virussurveysinoliveorchardsingreeceidentifyolivevirustanovelmemberofthegenustepovirus AT chatzivassiliouelisavetk virussurveysinoliveorchardsingreeceidentifyolivevirustanovelmemberofthegenustepovirus |