The Use of Molecular Profiling to Track Equine Reinfection Rates of Cyathostomin Species Following Anthelmintic Administration

SIMPLE SUMMARY: Cyathostomins (small strongyles) are a multispecies group of intestinal parasites in horses and the main target of deworming efforts by horse owners. It is not known whether species of cyathostomins have individual responses to dewormers. The objective of this study was to identify differences between cyathostomin species in reemergence rates following commercial dewormer treatment. This study used gene sequencing to profile the presence/absence of cyathostomin species in fecal samples at 2-week intervals following deworming to determine how quickly each species reinfected horses. Moxidectin was found to be the most effective at slowing the overall reemergence of these parasites, followed by Ivermectin, then Pyrantel. Seven species were resistant to all three deworming products. This study demonstrates that dewormer sensitivity differs between cyathostomin species, which could lead to more targeted control measures. ABSTRACT: Cyathostomins are a multispecies parasite ubiquitous in Equids. Cyathostomins have developed resistance to all but one class of anthelmintics, but species-level sensitivity to anthelmintics has not been shown. This study measured reinfection rates of cyathostomin species following the administration of three commercial dewormers. Nine treated horses were compared with 90 untreated controls during June-September 2017–2019. Ivermectin (IVM) (n = 6), Moxidectin (MOX) (n = 8) or Pyrantel (PYR) (n = 8) were orally administered. Fecal samples were collected every 14 d for 98 d. Fecal egg count reductions (FECR) were calculated using a modified McMaster technique. Nineteen cyathostomin species were identified by 5.8S-ITS-2 profiling using amplicon sequencing. Data were analyzed in QIIME1 and R statistical software using presence/absence methods. MOX had the lowest numbers of species present over the time course, followed by PYR then IVM (7.14, 10.17, 11.09, respectively); however, FECR was fastest for PYR. The presence of seven species: Coronocyclus labiatus, Cyathostomum catinatum, Cyathostomum tetracanthum, Cylicocylus elongatus, Cylicodontophorus bicoronatus, Cylicostephanus minutus, and Cylicostephanus goldi were unaffected by treatment (p > 0.05) points to species-specific differences in dewormer sensitivity and environmental persistence. Identifying resistance patterns at the species level will enable mechanistic understandings of cyathostomin anthelmintic resistance and targeted approaches to control them.