Cargando…
Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel
Wild barley is abundant, occupying large diversity of sites, ranging from the northern mesic Mediterranean meadows to the southern xeric deserts in Israel. This is also reflected in its wide phenotypic heterogeneity. We investigated the dynamics of DNA content changes in seed tissues in ten wild bar...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151103/ https://www.ncbi.nlm.nih.gov/pubmed/34068721 http://dx.doi.org/10.3390/genes12050711 |
_version_ | 1783698304746389504 |
---|---|
author | Nowicka, Anna Sahu, Pranav Pankaj Kovacik, Martin Weigt, Dorota Tokarz, Barbara Krugman, Tamar Pecinka, Ales |
author_facet | Nowicka, Anna Sahu, Pranav Pankaj Kovacik, Martin Weigt, Dorota Tokarz, Barbara Krugman, Tamar Pecinka, Ales |
author_sort | Nowicka, Anna |
collection | PubMed |
description | Wild barley is abundant, occupying large diversity of sites, ranging from the northern mesic Mediterranean meadows to the southern xeric deserts in Israel. This is also reflected in its wide phenotypic heterogeneity. We investigated the dynamics of DNA content changes in seed tissues in ten wild barley accessions that originated from an environmental gradient in Israel. The flow cytometric measurements were done from the time shortly after pollination up to the dry seeds. We show variation in mitotic cell cycle and endoreduplication dynamics in both diploid seed tissues (represented by seed maternal tissues and embryo) and in the triploid endosperm. We found that wild barley accessions collected at harsher xeric environmental conditions produce higher proportion of endoreduplicated nuclei in endosperm tissues. Also, a comparison of wild and cultivated barley strains revealed a higher endopolyploidy level in the endosperm of wild barley, that is accompanied by temporal changes in the timing of the major developmental phases. In summary, we present a new direction of research focusing on connecting spatiotemporal patterns of endoreduplication in barley seeds and possibly buffering for stress conditions. |
format | Online Article Text |
id | pubmed-8151103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81511032021-05-27 Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel Nowicka, Anna Sahu, Pranav Pankaj Kovacik, Martin Weigt, Dorota Tokarz, Barbara Krugman, Tamar Pecinka, Ales Genes (Basel) Article Wild barley is abundant, occupying large diversity of sites, ranging from the northern mesic Mediterranean meadows to the southern xeric deserts in Israel. This is also reflected in its wide phenotypic heterogeneity. We investigated the dynamics of DNA content changes in seed tissues in ten wild barley accessions that originated from an environmental gradient in Israel. The flow cytometric measurements were done from the time shortly after pollination up to the dry seeds. We show variation in mitotic cell cycle and endoreduplication dynamics in both diploid seed tissues (represented by seed maternal tissues and embryo) and in the triploid endosperm. We found that wild barley accessions collected at harsher xeric environmental conditions produce higher proportion of endoreduplicated nuclei in endosperm tissues. Also, a comparison of wild and cultivated barley strains revealed a higher endopolyploidy level in the endosperm of wild barley, that is accompanied by temporal changes in the timing of the major developmental phases. In summary, we present a new direction of research focusing on connecting spatiotemporal patterns of endoreduplication in barley seeds and possibly buffering for stress conditions. MDPI 2021-05-10 /pmc/articles/PMC8151103/ /pubmed/34068721 http://dx.doi.org/10.3390/genes12050711 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nowicka, Anna Sahu, Pranav Pankaj Kovacik, Martin Weigt, Dorota Tokarz, Barbara Krugman, Tamar Pecinka, Ales Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel |
title | Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel |
title_full | Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel |
title_fullStr | Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel |
title_full_unstemmed | Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel |
title_short | Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel |
title_sort | endopolyploidy variation in wild barley seeds across environmental gradients in israel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151103/ https://www.ncbi.nlm.nih.gov/pubmed/34068721 http://dx.doi.org/10.3390/genes12050711 |
work_keys_str_mv | AT nowickaanna endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael AT sahupranavpankaj endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael AT kovacikmartin endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael AT weigtdorota endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael AT tokarzbarbara endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael AT krugmantamar endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael AT pecinkaales endopolyploidyvariationinwildbarleyseedsacrossenvironmentalgradientsinisrael |