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Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells

Background: Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits...

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Autores principales: Vado, Yerai, Puras, Gustavo, Rosique, Melania, Martin, Cesar, Pedraz, Jose Luis, Jebari-Benslaiman, Shifa, de Pancorbo, Marian M., Zarate, Jon, Perez de Nanclares, Guiomar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151286/
https://www.ncbi.nlm.nih.gov/pubmed/34064902
http://dx.doi.org/10.3390/pharmaceutics13050696
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author Vado, Yerai
Puras, Gustavo
Rosique, Melania
Martin, Cesar
Pedraz, Jose Luis
Jebari-Benslaiman, Shifa
de Pancorbo, Marian M.
Zarate, Jon
Perez de Nanclares, Guiomar
author_facet Vado, Yerai
Puras, Gustavo
Rosique, Melania
Martin, Cesar
Pedraz, Jose Luis
Jebari-Benslaiman, Shifa
de Pancorbo, Marian M.
Zarate, Jon
Perez de Nanclares, Guiomar
author_sort Vado, Yerai
collection PubMed
description Background: Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits in the fact that they can be easily obtained noninvasively. Regarding gene delivery, nonviral vectors based on cationic niosomes have been used and are more stable and have lower immunogenicity than viral vectors. However, their transfection efficiency is low and in need of improvement. Methods: We isolated hUSCs from urine, and the cell culture was tested and characterized. Different cationic niosomes were elaborated using reverse-phase evaporation, and they were physicochemically characterized. Then, they were screened into hUSCs for transfection efficiency, and their internalization was evaluated. Results: GPxT-CQ at a lipid/DNA ratio of 5:1 (w/w) had the best transfection efficiency. Intracellular localization studies confirmed that nioplexes entered mainly via caveolae-mediated endocytosis. Conclusions: In conclusion, we established a protocol for hUSC isolation and their transfection with cationic niosomes, which could have relevant clinical applications such as in gene therapy. This methodology could also be used for creating cellular models for studying and validating pathogenic genetic variants, and even for performing functional studies. Our study increases knowledge about the internalization of tested cationic niosomes in these previously unexplored cells.
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spelling pubmed-81512862021-05-27 Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells Vado, Yerai Puras, Gustavo Rosique, Melania Martin, Cesar Pedraz, Jose Luis Jebari-Benslaiman, Shifa de Pancorbo, Marian M. Zarate, Jon Perez de Nanclares, Guiomar Pharmaceutics Article Background: Mesenchymal stem cells (MSCs) are stem cells present in adult tissues. They can be cultured, have great growth capacity, and can differentiate into several cell types. The isolation of urine-derived mesenchymal stem cells (hUSCs) was recently described. hUSCs present additional benefits in the fact that they can be easily obtained noninvasively. Regarding gene delivery, nonviral vectors based on cationic niosomes have been used and are more stable and have lower immunogenicity than viral vectors. However, their transfection efficiency is low and in need of improvement. Methods: We isolated hUSCs from urine, and the cell culture was tested and characterized. Different cationic niosomes were elaborated using reverse-phase evaporation, and they were physicochemically characterized. Then, they were screened into hUSCs for transfection efficiency, and their internalization was evaluated. Results: GPxT-CQ at a lipid/DNA ratio of 5:1 (w/w) had the best transfection efficiency. Intracellular localization studies confirmed that nioplexes entered mainly via caveolae-mediated endocytosis. Conclusions: In conclusion, we established a protocol for hUSC isolation and their transfection with cationic niosomes, which could have relevant clinical applications such as in gene therapy. This methodology could also be used for creating cellular models for studying and validating pathogenic genetic variants, and even for performing functional studies. Our study increases knowledge about the internalization of tested cationic niosomes in these previously unexplored cells. MDPI 2021-05-11 /pmc/articles/PMC8151286/ /pubmed/34064902 http://dx.doi.org/10.3390/pharmaceutics13050696 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vado, Yerai
Puras, Gustavo
Rosique, Melania
Martin, Cesar
Pedraz, Jose Luis
Jebari-Benslaiman, Shifa
de Pancorbo, Marian M.
Zarate, Jon
Perez de Nanclares, Guiomar
Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
title Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
title_full Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
title_fullStr Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
title_full_unstemmed Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
title_short Design and Validation of a Process Based on Cationic Niosomes for Gene Delivery into Novel Urine-Derived Mesenchymal Stem Cells
title_sort design and validation of a process based on cationic niosomes for gene delivery into novel urine-derived mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151286/
https://www.ncbi.nlm.nih.gov/pubmed/34064902
http://dx.doi.org/10.3390/pharmaceutics13050696
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