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Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)

Viral hemorrhagic septicemia (VHS), caused by viral hemorrhagic septicemia virus (VHSV), is a viral disease affecting teleosts, and is the major cause of virus-related deaths in olive flounder (Paralichthys olivaceus). Research has focused on ways to control VHS, and recently, the use of polyinosini...

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Autores principales: Chun, Jin Hong, Jung, Jae Wook, Kim, Young Rim, Lazarte, Jassy Mary S., Kim, Si Won, Kim, Jaesung, Thompson, Kim D., Kim, Hyoung Jun, Jung, Tae Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151365/
https://www.ncbi.nlm.nih.gov/pubmed/34068522
http://dx.doi.org/10.3390/vaccines9050482
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author Chun, Jin Hong
Jung, Jae Wook
Kim, Young Rim
Lazarte, Jassy Mary S.
Kim, Si Won
Kim, Jaesung
Thompson, Kim D.
Kim, Hyoung Jun
Jung, Tae Sung
author_facet Chun, Jin Hong
Jung, Jae Wook
Kim, Young Rim
Lazarte, Jassy Mary S.
Kim, Si Won
Kim, Jaesung
Thompson, Kim D.
Kim, Hyoung Jun
Jung, Tae Sung
author_sort Chun, Jin Hong
collection PubMed
description Viral hemorrhagic septicemia (VHS), caused by viral hemorrhagic septicemia virus (VHSV), is a viral disease affecting teleosts, and is the major cause of virus-related deaths in olive flounder (Paralichthys olivaceus). Research has focused on ways to control VHS, and recently, the use of polyinosinic-polycytidylic acid poly (I:C)-potentiated vaccination has been investigated, whereby fish are injected with poly (I:C) and then with live pathogenic virus, resulting in a significant decrease in VHSV-related mortality. T cell responses were investigated in the present study after vaccinating olive flounder with poly (I:C)-potentiated vaccination to understand the ability of poly (I:C) to induce T cell immunity. Stimulation of T cell responses with the poly (I:C)-potentiated vaccination was confirmed by examining levels of CD3(+) T cells, CD4-1(+) T cells and CD4-2(+) T cells. Higher levels of CD4-2(+) T cells were found in vaccinated fish than CD4-1(+) T cells, believed to result from a synergistic effect between poly (I:C) administration and pathogenic VHSV immunization. More importantly, the role of CD4-2(+) T cells in the antiviral response was clearly evident. The results of this study suggest that the outstanding protection obtained with the poly (I:C)-potentiated vaccination is due to the robust immune response initiated by the CD4-2(+) T cells.
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spelling pubmed-81513652021-05-27 Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV) Chun, Jin Hong Jung, Jae Wook Kim, Young Rim Lazarte, Jassy Mary S. Kim, Si Won Kim, Jaesung Thompson, Kim D. Kim, Hyoung Jun Jung, Tae Sung Vaccines (Basel) Article Viral hemorrhagic septicemia (VHS), caused by viral hemorrhagic septicemia virus (VHSV), is a viral disease affecting teleosts, and is the major cause of virus-related deaths in olive flounder (Paralichthys olivaceus). Research has focused on ways to control VHS, and recently, the use of polyinosinic-polycytidylic acid poly (I:C)-potentiated vaccination has been investigated, whereby fish are injected with poly (I:C) and then with live pathogenic virus, resulting in a significant decrease in VHSV-related mortality. T cell responses were investigated in the present study after vaccinating olive flounder with poly (I:C)-potentiated vaccination to understand the ability of poly (I:C) to induce T cell immunity. Stimulation of T cell responses with the poly (I:C)-potentiated vaccination was confirmed by examining levels of CD3(+) T cells, CD4-1(+) T cells and CD4-2(+) T cells. Higher levels of CD4-2(+) T cells were found in vaccinated fish than CD4-1(+) T cells, believed to result from a synergistic effect between poly (I:C) administration and pathogenic VHSV immunization. More importantly, the role of CD4-2(+) T cells in the antiviral response was clearly evident. The results of this study suggest that the outstanding protection obtained with the poly (I:C)-potentiated vaccination is due to the robust immune response initiated by the CD4-2(+) T cells. MDPI 2021-05-10 /pmc/articles/PMC8151365/ /pubmed/34068522 http://dx.doi.org/10.3390/vaccines9050482 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chun, Jin Hong
Jung, Jae Wook
Kim, Young Rim
Lazarte, Jassy Mary S.
Kim, Si Won
Kim, Jaesung
Thompson, Kim D.
Kim, Hyoung Jun
Jung, Tae Sung
Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)
title Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)
title_full Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)
title_fullStr Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)
title_full_unstemmed Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)
title_short Poly (I:C)-Potentiated Vaccination Enhances T Cell Response in Olive Flounder (Paralichthys olivaceus) Providing Protection against Viral Hemorrhagic Septicemia Virus (VHSV)
title_sort poly (i:c)-potentiated vaccination enhances t cell response in olive flounder (paralichthys olivaceus) providing protection against viral hemorrhagic septicemia virus (vhsv)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151365/
https://www.ncbi.nlm.nih.gov/pubmed/34068522
http://dx.doi.org/10.3390/vaccines9050482
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