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Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible man...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151575/ https://www.ncbi.nlm.nih.gov/pubmed/34064543 http://dx.doi.org/10.3390/s21103305 |
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author | Moreno, Sergio Canals, Joan Moro, Victor Franch, Nil Vilà, Anna Romano-Rodriguez, Albert Prades, Joan Daniel Bezshlyakh, Daria D. Waag, Andreas Kluczyk-Korch, Katarzyna Auf der Maur, Matthias Di Carlo, Aldo Krieger, Sigurd Geleff, Silvana Diéguez, Angel |
author_facet | Moreno, Sergio Canals, Joan Moro, Victor Franch, Nil Vilà, Anna Romano-Rodriguez, Albert Prades, Joan Daniel Bezshlyakh, Daria D. Waag, Andreas Kluczyk-Korch, Katarzyna Auf der Maur, Matthias Di Carlo, Aldo Krieger, Sigurd Geleff, Silvana Diéguez, Angel |
author_sort | Moreno, Sergio |
collection | PubMed |
description | Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible manner. This work presents the exploration of their capabilities with two different prototypes. In the first version, a resolution of less than 1 µm was shown thanks to a prototype based on an optically downscaled LED using an LED scanning transmission optical microscopy (STOM) technique. This research demonstrates how this technique can be used to improve STOM images by oversampling the acquisition. The second STOM-based microscope was fabricated with a 200 nm GaN LED. This demonstrates the possibilities for the miniaturization of on-chip-based microscopes. |
format | Online Article Text |
id | pubmed-8151575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81515752021-05-27 Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy Moreno, Sergio Canals, Joan Moro, Victor Franch, Nil Vilà, Anna Romano-Rodriguez, Albert Prades, Joan Daniel Bezshlyakh, Daria D. Waag, Andreas Kluczyk-Korch, Katarzyna Auf der Maur, Matthias Di Carlo, Aldo Krieger, Sigurd Geleff, Silvana Diéguez, Angel Sensors (Basel) Article Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible manner. This work presents the exploration of their capabilities with two different prototypes. In the first version, a resolution of less than 1 µm was shown thanks to a prototype based on an optically downscaled LED using an LED scanning transmission optical microscopy (STOM) technique. This research demonstrates how this technique can be used to improve STOM images by oversampling the acquisition. The second STOM-based microscope was fabricated with a 200 nm GaN LED. This demonstrates the possibilities for the miniaturization of on-chip-based microscopes. MDPI 2021-05-11 /pmc/articles/PMC8151575/ /pubmed/34064543 http://dx.doi.org/10.3390/s21103305 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moreno, Sergio Canals, Joan Moro, Victor Franch, Nil Vilà, Anna Romano-Rodriguez, Albert Prades, Joan Daniel Bezshlyakh, Daria D. Waag, Andreas Kluczyk-Korch, Katarzyna Auf der Maur, Matthias Di Carlo, Aldo Krieger, Sigurd Geleff, Silvana Diéguez, Angel Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy |
title | Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy |
title_full | Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy |
title_fullStr | Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy |
title_full_unstemmed | Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy |
title_short | Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy |
title_sort | pursuing the diffraction limit with nano-led scanning transmission optical microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151575/ https://www.ncbi.nlm.nih.gov/pubmed/34064543 http://dx.doi.org/10.3390/s21103305 |
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