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Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy

Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible man...

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Autores principales: Moreno, Sergio, Canals, Joan, Moro, Victor, Franch, Nil, Vilà, Anna, Romano-Rodriguez, Albert, Prades, Joan Daniel, Bezshlyakh, Daria D., Waag, Andreas, Kluczyk-Korch, Katarzyna, Auf der Maur, Matthias, Di Carlo, Aldo, Krieger, Sigurd, Geleff, Silvana, Diéguez, Angel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151575/
https://www.ncbi.nlm.nih.gov/pubmed/34064543
http://dx.doi.org/10.3390/s21103305
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author Moreno, Sergio
Canals, Joan
Moro, Victor
Franch, Nil
Vilà, Anna
Romano-Rodriguez, Albert
Prades, Joan Daniel
Bezshlyakh, Daria D.
Waag, Andreas
Kluczyk-Korch, Katarzyna
Auf der Maur, Matthias
Di Carlo, Aldo
Krieger, Sigurd
Geleff, Silvana
Diéguez, Angel
author_facet Moreno, Sergio
Canals, Joan
Moro, Victor
Franch, Nil
Vilà, Anna
Romano-Rodriguez, Albert
Prades, Joan Daniel
Bezshlyakh, Daria D.
Waag, Andreas
Kluczyk-Korch, Katarzyna
Auf der Maur, Matthias
Di Carlo, Aldo
Krieger, Sigurd
Geleff, Silvana
Diéguez, Angel
author_sort Moreno, Sergio
collection PubMed
description Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible manner. This work presents the exploration of their capabilities with two different prototypes. In the first version, a resolution of less than 1 µm was shown thanks to a prototype based on an optically downscaled LED using an LED scanning transmission optical microscopy (STOM) technique. This research demonstrates how this technique can be used to improve STOM images by oversampling the acquisition. The second STOM-based microscope was fabricated with a 200 nm GaN LED. This demonstrates the possibilities for the miniaturization of on-chip-based microscopes.
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spelling pubmed-81515752021-05-27 Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy Moreno, Sergio Canals, Joan Moro, Victor Franch, Nil Vilà, Anna Romano-Rodriguez, Albert Prades, Joan Daniel Bezshlyakh, Daria D. Waag, Andreas Kluczyk-Korch, Katarzyna Auf der Maur, Matthias Di Carlo, Aldo Krieger, Sigurd Geleff, Silvana Diéguez, Angel Sensors (Basel) Article Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible manner. This work presents the exploration of their capabilities with two different prototypes. In the first version, a resolution of less than 1 µm was shown thanks to a prototype based on an optically downscaled LED using an LED scanning transmission optical microscopy (STOM) technique. This research demonstrates how this technique can be used to improve STOM images by oversampling the acquisition. The second STOM-based microscope was fabricated with a 200 nm GaN LED. This demonstrates the possibilities for the miniaturization of on-chip-based microscopes. MDPI 2021-05-11 /pmc/articles/PMC8151575/ /pubmed/34064543 http://dx.doi.org/10.3390/s21103305 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moreno, Sergio
Canals, Joan
Moro, Victor
Franch, Nil
Vilà, Anna
Romano-Rodriguez, Albert
Prades, Joan Daniel
Bezshlyakh, Daria D.
Waag, Andreas
Kluczyk-Korch, Katarzyna
Auf der Maur, Matthias
Di Carlo, Aldo
Krieger, Sigurd
Geleff, Silvana
Diéguez, Angel
Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
title Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
title_full Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
title_fullStr Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
title_full_unstemmed Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
title_short Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy
title_sort pursuing the diffraction limit with nano-led scanning transmission optical microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151575/
https://www.ncbi.nlm.nih.gov/pubmed/34064543
http://dx.doi.org/10.3390/s21103305
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