Cargando…

Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7

Circular RNAs (circRNAs) represent a class of covalently closed single-stranded RNA molecules that are emerging as essential regulators of various biological processes. The circRNA circHipk2 originates from exon 2 of the Hipk2 gene in mice and was reported to be involved in acute promyelocytic leuke...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Junyu, Yang, Yalan, Fan, Xinhao, Tang, Yijie, Tang, Zhonglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151578/
https://www.ncbi.nlm.nih.gov/pubmed/34066653
http://dx.doi.org/10.3390/genes12050696
_version_ 1783698416299147264
author Yan, Junyu
Yang, Yalan
Fan, Xinhao
Tang, Yijie
Tang, Zhonglin
author_facet Yan, Junyu
Yang, Yalan
Fan, Xinhao
Tang, Yijie
Tang, Zhonglin
author_sort Yan, Junyu
collection PubMed
description Circular RNAs (circRNAs) represent a class of covalently closed single-stranded RNA molecules that are emerging as essential regulators of various biological processes. The circRNA circHipk2 originates from exon 2 of the Hipk2 gene in mice and was reported to be involved in acute promyelocytic leukemia and myocardial injury. However, the functions and mechanisms of circHipk2 in myogenesis are largely unknown. Here, to deepen our knowledge about the role of circHipk2, we studied the expression and function of circHipk2 during skeletal myogenesis. We found that circHipk2 was mostly distributed in the cytoplasm, and dynamically and differentially expressed in various myogenesis systems in vitro and in vivo. Functionally, overexpression of circHipk2 inhibited myoblast proliferation and promoted myotube formation in C2C12 cells, whereas the opposite effects were observed after circHipk2 knockdown. Mechanistically, circHipk2 could directly bind to ribosomal protein Rpl7, an essential 60S preribosomal assembly factor, to inhibit ribosome translation. In addition, we verified that transcription factor Sp1 directly bound to the promoter of circHipk2 and affected the expression of Hipk2 and circHipk2 in C2C12 myoblasts. Collectively, these findings identify circHipk2 as a candidate circRNA regulating ribosome biogenesis and myogenesis proliferation and differentiation.
format Online
Article
Text
id pubmed-8151578
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81515782021-05-27 Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7 Yan, Junyu Yang, Yalan Fan, Xinhao Tang, Yijie Tang, Zhonglin Genes (Basel) Article Circular RNAs (circRNAs) represent a class of covalently closed single-stranded RNA molecules that are emerging as essential regulators of various biological processes. The circRNA circHipk2 originates from exon 2 of the Hipk2 gene in mice and was reported to be involved in acute promyelocytic leukemia and myocardial injury. However, the functions and mechanisms of circHipk2 in myogenesis are largely unknown. Here, to deepen our knowledge about the role of circHipk2, we studied the expression and function of circHipk2 during skeletal myogenesis. We found that circHipk2 was mostly distributed in the cytoplasm, and dynamically and differentially expressed in various myogenesis systems in vitro and in vivo. Functionally, overexpression of circHipk2 inhibited myoblast proliferation and promoted myotube formation in C2C12 cells, whereas the opposite effects were observed after circHipk2 knockdown. Mechanistically, circHipk2 could directly bind to ribosomal protein Rpl7, an essential 60S preribosomal assembly factor, to inhibit ribosome translation. In addition, we verified that transcription factor Sp1 directly bound to the promoter of circHipk2 and affected the expression of Hipk2 and circHipk2 in C2C12 myoblasts. Collectively, these findings identify circHipk2 as a candidate circRNA regulating ribosome biogenesis and myogenesis proliferation and differentiation. MDPI 2021-05-08 /pmc/articles/PMC8151578/ /pubmed/34066653 http://dx.doi.org/10.3390/genes12050696 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Junyu
Yang, Yalan
Fan, Xinhao
Tang, Yijie
Tang, Zhonglin
Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
title Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
title_full Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
title_fullStr Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
title_full_unstemmed Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
title_short Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
title_sort sp1-mediated circrna circhipk2 regulates myogenesis by targeting ribosomal protein rpl7
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151578/
https://www.ncbi.nlm.nih.gov/pubmed/34066653
http://dx.doi.org/10.3390/genes12050696
work_keys_str_mv AT yanjunyu sp1mediatedcircrnacirchipk2regulatesmyogenesisbytargetingribosomalproteinrpl7
AT yangyalan sp1mediatedcircrnacirchipk2regulatesmyogenesisbytargetingribosomalproteinrpl7
AT fanxinhao sp1mediatedcircrnacirchipk2regulatesmyogenesisbytargetingribosomalproteinrpl7
AT tangyijie sp1mediatedcircrnacirchipk2regulatesmyogenesisbytargetingribosomalproteinrpl7
AT tangzhonglin sp1mediatedcircrnacirchipk2regulatesmyogenesisbytargetingribosomalproteinrpl7