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Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo

Echinacea purpurea L. (Moench) is used in traditional and conventional medicine. However, there is lack of data on the biological activities of primary plant metabolite lectins. The aim of our experiment was to find out how lectin LysM (lysine motif), which was previously purified, affects the immun...

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Autores principales: Balčiūnaitė-Murzienė, Gabrielė, Miknienė, Zoja, Ragažinskienė, Ona, Juodžiukynienė, Nomeda, Savickas, Arūnas, Savickienė, Nijolė, Pangonytė, Dalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151625/
https://www.ncbi.nlm.nih.gov/pubmed/34067195
http://dx.doi.org/10.3390/plants10050936
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author Balčiūnaitė-Murzienė, Gabrielė
Miknienė, Zoja
Ragažinskienė, Ona
Juodžiukynienė, Nomeda
Savickas, Arūnas
Savickienė, Nijolė
Pangonytė, Dalia
author_facet Balčiūnaitė-Murzienė, Gabrielė
Miknienė, Zoja
Ragažinskienė, Ona
Juodžiukynienė, Nomeda
Savickas, Arūnas
Savickienė, Nijolė
Pangonytė, Dalia
author_sort Balčiūnaitė-Murzienė, Gabrielė
collection PubMed
description Echinacea purpurea L. (Moench) is used in traditional and conventional medicine. However, there is lack of data on the biological activities of primary plant metabolite lectins. The aim of our experiment was to find out how lectin LysM (lysine motif), which was previously purified, affects the immune response in vivo. Eight-week-old BALB/c male mice (n = 15) received four weekly 250 μg/kg peritonial injections of purified Echinacea purpurea L. (Moench) roots’ LysM lectin. The control animal group (n = 15) received 50 μL peritoneal injections of fresh Echinacea purpurea L. (Moench) root tincture, and the negative control animal group (n = 15) received 50 μL peritoneal injections of physiological solution. At the fifth experimental week, the animals were sedated with carbon dioxide, and later euthanized by cervical dislocation, and then their blood and spleen samples were collected. The leukocytes’ formula and lymphocytes’ count was estimated in blood samples, the T lymphocytes’ density was evaluated in spleen zones. A statistically significant (p < 0.05) difference between each group was observed in the leukocytes’ formula (monocytes’ percentage, also little, medium and giant size lymphocytes). The purple coneflower fresh roots’ tincture significantly decreased (p < 0.05) the T lymphocytes’ quantity in peritoneal lymphoid sheaths (PALS) compared with the physiological solution injection’s group (p < 0.05) and the lectin injection’s group (p < 0.001). Meanwhile, lectin injections caused a significant (p < 0.01) increase in the T lymphocytes in a spleen PALS zone, compared with the physiological solution and tincture injection’s group. Our data suggests that LysM lectin acts as an immunostimulant, while fresh purple coneflower tincture causes immunosuppression.
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spelling pubmed-81516252021-05-27 Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo Balčiūnaitė-Murzienė, Gabrielė Miknienė, Zoja Ragažinskienė, Ona Juodžiukynienė, Nomeda Savickas, Arūnas Savickienė, Nijolė Pangonytė, Dalia Plants (Basel) Article Echinacea purpurea L. (Moench) is used in traditional and conventional medicine. However, there is lack of data on the biological activities of primary plant metabolite lectins. The aim of our experiment was to find out how lectin LysM (lysine motif), which was previously purified, affects the immune response in vivo. Eight-week-old BALB/c male mice (n = 15) received four weekly 250 μg/kg peritonial injections of purified Echinacea purpurea L. (Moench) roots’ LysM lectin. The control animal group (n = 15) received 50 μL peritoneal injections of fresh Echinacea purpurea L. (Moench) root tincture, and the negative control animal group (n = 15) received 50 μL peritoneal injections of physiological solution. At the fifth experimental week, the animals were sedated with carbon dioxide, and later euthanized by cervical dislocation, and then their blood and spleen samples were collected. The leukocytes’ formula and lymphocytes’ count was estimated in blood samples, the T lymphocytes’ density was evaluated in spleen zones. A statistically significant (p < 0.05) difference between each group was observed in the leukocytes’ formula (monocytes’ percentage, also little, medium and giant size lymphocytes). The purple coneflower fresh roots’ tincture significantly decreased (p < 0.05) the T lymphocytes’ quantity in peritoneal lymphoid sheaths (PALS) compared with the physiological solution injection’s group (p < 0.05) and the lectin injection’s group (p < 0.001). Meanwhile, lectin injections caused a significant (p < 0.01) increase in the T lymphocytes in a spleen PALS zone, compared with the physiological solution and tincture injection’s group. Our data suggests that LysM lectin acts as an immunostimulant, while fresh purple coneflower tincture causes immunosuppression. MDPI 2021-05-07 /pmc/articles/PMC8151625/ /pubmed/34067195 http://dx.doi.org/10.3390/plants10050936 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Balčiūnaitė-Murzienė, Gabrielė
Miknienė, Zoja
Ragažinskienė, Ona
Juodžiukynienė, Nomeda
Savickas, Arūnas
Savickienė, Nijolė
Pangonytė, Dalia
Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo
title Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo
title_full Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo
title_fullStr Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo
title_full_unstemmed Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo
title_short Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo
title_sort echinacea purpurea l. (moench) hemagglutinin effect on immune response in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151625/
https://www.ncbi.nlm.nih.gov/pubmed/34067195
http://dx.doi.org/10.3390/plants10050936
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