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Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro
Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and differentiation. In vitro cell culture, however,...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151809/ https://www.ncbi.nlm.nih.gov/pubmed/34066027 http://dx.doi.org/10.3390/cells10051177 |
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author | Hunter-Featherstone, Eve Young, Natalie Chamberlain, Kathryn Cubillas, Pablo Hulette, Ben Wei, Xingtao Tiesman, Jay P. Bascom, Charles C. Benham, Adam M. Goldberg, Martin W. Saretzki, Gabriele Karakesisoglou, Iakowos |
author_facet | Hunter-Featherstone, Eve Young, Natalie Chamberlain, Kathryn Cubillas, Pablo Hulette, Ben Wei, Xingtao Tiesman, Jay P. Bascom, Charles C. Benham, Adam M. Goldberg, Martin W. Saretzki, Gabriele Karakesisoglou, Iakowos |
author_sort | Hunter-Featherstone, Eve |
collection | PubMed |
description | Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and differentiation. In vitro cell culture, however, utilises tissue culture plastic, which is significantly stiffer than the in vivo environment. Current epidermal models fail to consider the effects of culturing keratinocytes on plastic prior to setting up three-dimensional cultures, so the impact of this non-physiological exposure on epidermal assembly is largely overlooked. In this study, primary keratinocytes cultured on plastic were compared with those grown on 4, 8, and 50 kPa stiff biomimetic hydrogels that have similar mechanical properties to skin. Our data show that keratinocytes cultured on biomimetic hydrogels exhibited major changes in cellular architecture, cell density, nuclear biomechanics, and mechanoprotein expression, such as specific Linker of Nucleoskeleton and Cytoskeleton (LINC) complex constituents. Mechanical conditioning of keratinocytes on 50 kPa biomimetic hydrogels improved the thickness and organisation of 3D epidermal models. In summary, the current study demonstrates that the effects of extracellular mechanics on keratinocyte cell biology are significant and therefore should be harnessed in skin research to ensure the successful production of physiologically relevant skin models. |
format | Online Article Text |
id | pubmed-8151809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81518092021-05-27 Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro Hunter-Featherstone, Eve Young, Natalie Chamberlain, Kathryn Cubillas, Pablo Hulette, Ben Wei, Xingtao Tiesman, Jay P. Bascom, Charles C. Benham, Adam M. Goldberg, Martin W. Saretzki, Gabriele Karakesisoglou, Iakowos Cells Article Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond to mechanical cues by altering their proliferation, migration, and differentiation. In vitro cell culture, however, utilises tissue culture plastic, which is significantly stiffer than the in vivo environment. Current epidermal models fail to consider the effects of culturing keratinocytes on plastic prior to setting up three-dimensional cultures, so the impact of this non-physiological exposure on epidermal assembly is largely overlooked. In this study, primary keratinocytes cultured on plastic were compared with those grown on 4, 8, and 50 kPa stiff biomimetic hydrogels that have similar mechanical properties to skin. Our data show that keratinocytes cultured on biomimetic hydrogels exhibited major changes in cellular architecture, cell density, nuclear biomechanics, and mechanoprotein expression, such as specific Linker of Nucleoskeleton and Cytoskeleton (LINC) complex constituents. Mechanical conditioning of keratinocytes on 50 kPa biomimetic hydrogels improved the thickness and organisation of 3D epidermal models. In summary, the current study demonstrates that the effects of extracellular mechanics on keratinocyte cell biology are significant and therefore should be harnessed in skin research to ensure the successful production of physiologically relevant skin models. MDPI 2021-05-12 /pmc/articles/PMC8151809/ /pubmed/34066027 http://dx.doi.org/10.3390/cells10051177 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hunter-Featherstone, Eve Young, Natalie Chamberlain, Kathryn Cubillas, Pablo Hulette, Ben Wei, Xingtao Tiesman, Jay P. Bascom, Charles C. Benham, Adam M. Goldberg, Martin W. Saretzki, Gabriele Karakesisoglou, Iakowos Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro |
title | Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro |
title_full | Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro |
title_fullStr | Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro |
title_full_unstemmed | Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro |
title_short | Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro |
title_sort | culturing keratinocytes on biomimetic substrates facilitates improved epidermal assembly in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151809/ https://www.ncbi.nlm.nih.gov/pubmed/34066027 http://dx.doi.org/10.3390/cells10051177 |
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