Cargando…

Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation

Environmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compo...

Descripción completa

Detalles Bibliográficos
Autores principales: Baron, Noemi Carla, Pagnocca, Fernando Carlos, Otsuka, Ayumi Aquino, Prenafeta-Boldú, Francesc Xavier, Vicente, Vânia Aparecida, Attili de Angelis, Derlene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151820/
https://www.ncbi.nlm.nih.gov/pubmed/34067085
http://dx.doi.org/10.3390/microorganisms9051008
_version_ 1783698473593339904
author Baron, Noemi Carla
Pagnocca, Fernando Carlos
Otsuka, Ayumi Aquino
Prenafeta-Boldú, Francesc Xavier
Vicente, Vânia Aparecida
Attili de Angelis, Derlene
author_facet Baron, Noemi Carla
Pagnocca, Fernando Carlos
Otsuka, Ayumi Aquino
Prenafeta-Boldú, Francesc Xavier
Vicente, Vânia Aparecida
Attili de Angelis, Derlene
author_sort Baron, Noemi Carla
collection PubMed
description Environmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compounds. Melanized fungi present extremophilic characteristics, which allow their survival in inhospitable habitats such as those contaminated with hydrocarbons. Screening methodologies for testing the microbial assimilation of volatile organic compounds (VOC) are scarce despite their importance for the bioremediation of hydrocarbon associated areas. In this study, 200 strains of melanized fungi were isolated from four different hydrocarbon-related environments by using selective methods, and their biodiversity was assessed by molecular and ecological analyses. Seventeen genera and 27 species from three main orders, namely Chaetothyriales, Cladosporiales, and Pleosporales, were identified. The ecological analysis showed a particular species distribution according to their original substrate. The isolated strains were also screened for their toluene assimilation potential using a simple and inexpensive methodology based on miniaturized incubations under controlled atmospheres. The biomass produced by the 200 strains with toluene as the sole carbon source was compared against positive and negative controls, with glucose and with only mineral medium, respectively. Nineteen strains were selected as the most promising for further investigation on the biodegradation of alkylbenzenes.
format Online
Article
Text
id pubmed-8151820
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81518202021-05-27 Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation Baron, Noemi Carla Pagnocca, Fernando Carlos Otsuka, Ayumi Aquino Prenafeta-Boldú, Francesc Xavier Vicente, Vânia Aparecida Attili de Angelis, Derlene Microorganisms Article Environmental pollution with alkylbenzene hydrocarbons such as toluene is a recurring phenomenon. Their toxicity and harmful effect on people and the environment drive the search for sustainable removal techniques such as bioremediation, which is based on the microbial metabolism of xenobiotic compounds. Melanized fungi present extremophilic characteristics, which allow their survival in inhospitable habitats such as those contaminated with hydrocarbons. Screening methodologies for testing the microbial assimilation of volatile organic compounds (VOC) are scarce despite their importance for the bioremediation of hydrocarbon associated areas. In this study, 200 strains of melanized fungi were isolated from four different hydrocarbon-related environments by using selective methods, and their biodiversity was assessed by molecular and ecological analyses. Seventeen genera and 27 species from three main orders, namely Chaetothyriales, Cladosporiales, and Pleosporales, were identified. The ecological analysis showed a particular species distribution according to their original substrate. The isolated strains were also screened for their toluene assimilation potential using a simple and inexpensive methodology based on miniaturized incubations under controlled atmospheres. The biomass produced by the 200 strains with toluene as the sole carbon source was compared against positive and negative controls, with glucose and with only mineral medium, respectively. Nineteen strains were selected as the most promising for further investigation on the biodegradation of alkylbenzenes. MDPI 2021-05-07 /pmc/articles/PMC8151820/ /pubmed/34067085 http://dx.doi.org/10.3390/microorganisms9051008 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baron, Noemi Carla
Pagnocca, Fernando Carlos
Otsuka, Ayumi Aquino
Prenafeta-Boldú, Francesc Xavier
Vicente, Vânia Aparecida
Attili de Angelis, Derlene
Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation
title Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation
title_full Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation
title_fullStr Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation
title_full_unstemmed Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation
title_short Black Fungi and Hydrocarbons: An Environmental Survey for Alkylbenzene Assimilation
title_sort black fungi and hydrocarbons: an environmental survey for alkylbenzene assimilation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151820/
https://www.ncbi.nlm.nih.gov/pubmed/34067085
http://dx.doi.org/10.3390/microorganisms9051008
work_keys_str_mv AT baronnoemicarla blackfungiandhydrocarbonsanenvironmentalsurveyforalkylbenzeneassimilation
AT pagnoccafernandocarlos blackfungiandhydrocarbonsanenvironmentalsurveyforalkylbenzeneassimilation
AT otsukaayumiaquino blackfungiandhydrocarbonsanenvironmentalsurveyforalkylbenzeneassimilation
AT prenafetaboldufrancescxavier blackfungiandhydrocarbonsanenvironmentalsurveyforalkylbenzeneassimilation
AT vicentevaniaaparecida blackfungiandhydrocarbonsanenvironmentalsurveyforalkylbenzeneassimilation
AT attilideangelisderlene blackfungiandhydrocarbonsanenvironmentalsurveyforalkylbenzeneassimilation