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Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens
The virological meaning of the different patterns of serology in COVID-19 has been little examined in clinical settings. Asymptomatic subjects with IgM-spike (S) and IgG-nucleocapsid (N) determinations by chemiluminescence were studied for SARS-CoV-2 shedding in respiratory secretions by transcripti...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151912/ https://www.ncbi.nlm.nih.gov/pubmed/34068703 http://dx.doi.org/10.3390/v13050874 |
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author | Barreiro, Pablo Candel, Francisco Javier Sanz, Juan Carlos San Román, Jesús del Mar Carretero, María Pérez-Abeledo, Marta Ramos, Belén Viñuela-Prieto, José Manuel Canora, Jesús Martínez-Peromingo, Francisco Javier Zapatero, Antonio |
author_facet | Barreiro, Pablo Candel, Francisco Javier Sanz, Juan Carlos San Román, Jesús del Mar Carretero, María Pérez-Abeledo, Marta Ramos, Belén Viñuela-Prieto, José Manuel Canora, Jesús Martínez-Peromingo, Francisco Javier Zapatero, Antonio |
author_sort | Barreiro, Pablo |
collection | PubMed |
description | The virological meaning of the different patterns of serology in COVID-19 has been little examined in clinical settings. Asymptomatic subjects with IgM-spike (S) and IgG-nucleocapsid (N) determinations by chemiluminescence were studied for SARS-CoV-2 shedding in respiratory secretions by transcription-mediated amplification (TMA). In subjects showing IgM-S positive and IgG-N negative, IgG-S was determined by lateral flow assay. A total of 712 individuals were tested: 30.0% presented IgM-S(+)/IgG-N(−), 25.8% had IgM-S(+)/IgG-N(+) and 44.2% had IgM-S(−)/IgG-N(+); the proportion with TMA(+) were comparable in these three groups: 12.1, 8.7 and 10.5%, respectively. In individuals with IgM-S(+)/IgG-N(−), IgG-S(+) was detected in 66.5%. The frequency of IgM-S(+)/IgG-S(−) in the total population was 10.0%, of whom 24.1% had TMA(+); the chances for TMA(+) in subjects with an IgM-S(+) alone pattern were 2.4%. Targeting of the same SARS-CoV-2 antigen seems to be better for the characterization of IgM/IgG patterns of response. IgM-S(+) alone reactivity is rare, and a small proportion is associated with viral shedding. |
format | Online Article Text |
id | pubmed-8151912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81519122021-05-27 Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens Barreiro, Pablo Candel, Francisco Javier Sanz, Juan Carlos San Román, Jesús del Mar Carretero, María Pérez-Abeledo, Marta Ramos, Belén Viñuela-Prieto, José Manuel Canora, Jesús Martínez-Peromingo, Francisco Javier Zapatero, Antonio Viruses Article The virological meaning of the different patterns of serology in COVID-19 has been little examined in clinical settings. Asymptomatic subjects with IgM-spike (S) and IgG-nucleocapsid (N) determinations by chemiluminescence were studied for SARS-CoV-2 shedding in respiratory secretions by transcription-mediated amplification (TMA). In subjects showing IgM-S positive and IgG-N negative, IgG-S was determined by lateral flow assay. A total of 712 individuals were tested: 30.0% presented IgM-S(+)/IgG-N(−), 25.8% had IgM-S(+)/IgG-N(+) and 44.2% had IgM-S(−)/IgG-N(+); the proportion with TMA(+) were comparable in these three groups: 12.1, 8.7 and 10.5%, respectively. In individuals with IgM-S(+)/IgG-N(−), IgG-S(+) was detected in 66.5%. The frequency of IgM-S(+)/IgG-S(−) in the total population was 10.0%, of whom 24.1% had TMA(+); the chances for TMA(+) in subjects with an IgM-S(+) alone pattern were 2.4%. Targeting of the same SARS-CoV-2 antigen seems to be better for the characterization of IgM/IgG patterns of response. IgM-S(+) alone reactivity is rare, and a small proportion is associated with viral shedding. MDPI 2021-05-10 /pmc/articles/PMC8151912/ /pubmed/34068703 http://dx.doi.org/10.3390/v13050874 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Barreiro, Pablo Candel, Francisco Javier Sanz, Juan Carlos San Román, Jesús del Mar Carretero, María Pérez-Abeledo, Marta Ramos, Belén Viñuela-Prieto, José Manuel Canora, Jesús Martínez-Peromingo, Francisco Javier Zapatero, Antonio Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens |
title | Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens |
title_full | Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens |
title_fullStr | Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens |
title_full_unstemmed | Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens |
title_short | Virological Correlates of IgM–IgG Patterns of Response to SARS-CoV-2 Infection According to Targeted Antigens |
title_sort | virological correlates of igm–igg patterns of response to sars-cov-2 infection according to targeted antigens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151912/ https://www.ncbi.nlm.nih.gov/pubmed/34068703 http://dx.doi.org/10.3390/v13050874 |
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