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Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans

Various peptides and their derivatives have been reported to exhibit antimicrobial activities. Although these activities have been examined against microorganisms, novel methods have recently emerged for conjugation of the biomaterials to improve their activities. Here, we prepared CKR12-PLGA, in wh...

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Autores principales: Mori, Takeshi, Yoshida, Miyako, Hazekawa, Mai, Ishibashi, Daisuke, Hatanaka, Yoshiro, Nagao, Toshihiro, Kakehashi, Rie, Kojima, Honami, Uno, Rio, Ozeki, Minoru, Kawasaki, Ikuo, Yamashita, Taku, Nishikawa, Junichi, Uchida, Takahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151943/
https://www.ncbi.nlm.nih.gov/pubmed/34065861
http://dx.doi.org/10.3390/ijms22105097
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author Mori, Takeshi
Yoshida, Miyako
Hazekawa, Mai
Ishibashi, Daisuke
Hatanaka, Yoshiro
Nagao, Toshihiro
Kakehashi, Rie
Kojima, Honami
Uno, Rio
Ozeki, Minoru
Kawasaki, Ikuo
Yamashita, Taku
Nishikawa, Junichi
Uchida, Takahiro
author_facet Mori, Takeshi
Yoshida, Miyako
Hazekawa, Mai
Ishibashi, Daisuke
Hatanaka, Yoshiro
Nagao, Toshihiro
Kakehashi, Rie
Kojima, Honami
Uno, Rio
Ozeki, Minoru
Kawasaki, Ikuo
Yamashita, Taku
Nishikawa, Junichi
Uchida, Takahiro
author_sort Mori, Takeshi
collection PubMed
description Various peptides and their derivatives have been reported to exhibit antimicrobial activities. Although these activities have been examined against microorganisms, novel methods have recently emerged for conjugation of the biomaterials to improve their activities. Here, we prepared CKR12-PLGA, in which CKR12 (a mutated fragment of human cathelicidin peptide, LL-37) was conjugated with poly (lactic-co-glycolic) acid (PLGA), and compared the antimicrobial and antifungal activities of the conjugated peptide with those of FK13 (a small fragment of LL-37) and CKR12 alone. The prepared CKR12-PLGA was characterized by dynamic light scattering and measurement of the zeta potential, critical micellar concentration, and antimicrobial activities of the fragments and conjugate. Although CKR12 showed higher antibacterial activities than FK13 against Staphylococcus aureus and Escherichia coli, the antifungal activity of CKR12 was lower than that of FK13. CKR12-PLGA showed higher antibacterial activities against S. aureus and E. coli and higher antifungal activity against Candida albicans compared to those of FK13. Additionally, CKR12-PLGA showed no hemolytic activity in erythrocytes, and scanning and transmission electron microscopy suggested that CKR12-PLGA killed and disrupted the surface structure of microbial cells. Conjugation of antimicrobial peptide fragment analogues was a successful approach for obtaining increased microbial activity with minimized cytotoxicity.
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spelling pubmed-81519432021-05-27 Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans Mori, Takeshi Yoshida, Miyako Hazekawa, Mai Ishibashi, Daisuke Hatanaka, Yoshiro Nagao, Toshihiro Kakehashi, Rie Kojima, Honami Uno, Rio Ozeki, Minoru Kawasaki, Ikuo Yamashita, Taku Nishikawa, Junichi Uchida, Takahiro Int J Mol Sci Article Various peptides and their derivatives have been reported to exhibit antimicrobial activities. Although these activities have been examined against microorganisms, novel methods have recently emerged for conjugation of the biomaterials to improve their activities. Here, we prepared CKR12-PLGA, in which CKR12 (a mutated fragment of human cathelicidin peptide, LL-37) was conjugated with poly (lactic-co-glycolic) acid (PLGA), and compared the antimicrobial and antifungal activities of the conjugated peptide with those of FK13 (a small fragment of LL-37) and CKR12 alone. The prepared CKR12-PLGA was characterized by dynamic light scattering and measurement of the zeta potential, critical micellar concentration, and antimicrobial activities of the fragments and conjugate. Although CKR12 showed higher antibacterial activities than FK13 against Staphylococcus aureus and Escherichia coli, the antifungal activity of CKR12 was lower than that of FK13. CKR12-PLGA showed higher antibacterial activities against S. aureus and E. coli and higher antifungal activity against Candida albicans compared to those of FK13. Additionally, CKR12-PLGA showed no hemolytic activity in erythrocytes, and scanning and transmission electron microscopy suggested that CKR12-PLGA killed and disrupted the surface structure of microbial cells. Conjugation of antimicrobial peptide fragment analogues was a successful approach for obtaining increased microbial activity with minimized cytotoxicity. MDPI 2021-05-12 /pmc/articles/PMC8151943/ /pubmed/34065861 http://dx.doi.org/10.3390/ijms22105097 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mori, Takeshi
Yoshida, Miyako
Hazekawa, Mai
Ishibashi, Daisuke
Hatanaka, Yoshiro
Nagao, Toshihiro
Kakehashi, Rie
Kojima, Honami
Uno, Rio
Ozeki, Minoru
Kawasaki, Ikuo
Yamashita, Taku
Nishikawa, Junichi
Uchida, Takahiro
Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans
title Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans
title_full Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans
title_fullStr Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans
title_full_unstemmed Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans
title_short Antimicrobial Activities of LL-37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate against Staphylococcus aureus, Escherichia coli, and Candida albicans
title_sort antimicrobial activities of ll-37 fragment mutant-poly (lactic-co-glycolic) acid conjugate against staphylococcus aureus, escherichia coli, and candida albicans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151943/
https://www.ncbi.nlm.nih.gov/pubmed/34065861
http://dx.doi.org/10.3390/ijms22105097
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