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Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles

The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two a...

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Autores principales: Sabio, Laura, Sosa, Andrea, Delgado-López, José M., Dominguez-Vera, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151946/
https://www.ncbi.nlm.nih.gov/pubmed/34064907
http://dx.doi.org/10.3390/molecules26102848
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author Sabio, Laura
Sosa, Andrea
Delgado-López, José M.
Dominguez-Vera, José M.
author_facet Sabio, Laura
Sosa, Andrea
Delgado-López, José M.
Dominguez-Vera, José M.
author_sort Sabio, Laura
collection PubMed
description The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two antibacterials, silver nanoparticles (AgNPs) and living probiotics (Lactobacillus fermentum, Lf), using bacterial cellulose (BC) as scaffold. By controlling the loading of each antibacterial at opposite BC sides, we obtained a two-sided biomaterial (AgNP-BC-Lf) with a high density of alive and metabolically active probiotics on one surface and AgNPs on the opposite one, being probiotics well preserved from the killer effect of AgNPs. The resulting two-sided biomaterial was characterized by Field-Emission Scanning Electron Microscopy (FESEM) and Confocal Laser Scanning Microscopy (CLSM). The antibacterial capacity against Pseudomonas aeruginosa (PA), an opportunistic pathogen responsible for a broad range of skin infections, was also assessed by agar diffusion tests in pathogen-favorable media. Results showed an enhanced activity against PA when both antibacterials were combined into BC (AgNP-BC-Lf) with respect to BC containing only one of the antibacterials, BC-Lf or AgNP-BC. Therefore, AgNP-BC-Lf is an antibiotic-free biomaterial that can be useful for the therapy of topical bacterial infections.
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spelling pubmed-81519462021-05-27 Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles Sabio, Laura Sosa, Andrea Delgado-López, José M. Dominguez-Vera, José M. Molecules Communication The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two antibacterials, silver nanoparticles (AgNPs) and living probiotics (Lactobacillus fermentum, Lf), using bacterial cellulose (BC) as scaffold. By controlling the loading of each antibacterial at opposite BC sides, we obtained a two-sided biomaterial (AgNP-BC-Lf) with a high density of alive and metabolically active probiotics on one surface and AgNPs on the opposite one, being probiotics well preserved from the killer effect of AgNPs. The resulting two-sided biomaterial was characterized by Field-Emission Scanning Electron Microscopy (FESEM) and Confocal Laser Scanning Microscopy (CLSM). The antibacterial capacity against Pseudomonas aeruginosa (PA), an opportunistic pathogen responsible for a broad range of skin infections, was also assessed by agar diffusion tests in pathogen-favorable media. Results showed an enhanced activity against PA when both antibacterials were combined into BC (AgNP-BC-Lf) with respect to BC containing only one of the antibacterials, BC-Lf or AgNP-BC. Therefore, AgNP-BC-Lf is an antibiotic-free biomaterial that can be useful for the therapy of topical bacterial infections. MDPI 2021-05-11 /pmc/articles/PMC8151946/ /pubmed/34064907 http://dx.doi.org/10.3390/molecules26102848 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Sabio, Laura
Sosa, Andrea
Delgado-López, José M.
Dominguez-Vera, José M.
Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
title Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
title_full Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
title_fullStr Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
title_full_unstemmed Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
title_short Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
title_sort two-sided antibacterial cellulose combining probiotics and silver nanoparticles
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151946/
https://www.ncbi.nlm.nih.gov/pubmed/34064907
http://dx.doi.org/10.3390/molecules26102848
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AT dominguezverajosem twosidedantibacterialcellulosecombiningprobioticsandsilvernanoparticles