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Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles
The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151946/ https://www.ncbi.nlm.nih.gov/pubmed/34064907 http://dx.doi.org/10.3390/molecules26102848 |
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author | Sabio, Laura Sosa, Andrea Delgado-López, José M. Dominguez-Vera, José M. |
author_facet | Sabio, Laura Sosa, Andrea Delgado-López, José M. Dominguez-Vera, José M. |
author_sort | Sabio, Laura |
collection | PubMed |
description | The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two antibacterials, silver nanoparticles (AgNPs) and living probiotics (Lactobacillus fermentum, Lf), using bacterial cellulose (BC) as scaffold. By controlling the loading of each antibacterial at opposite BC sides, we obtained a two-sided biomaterial (AgNP-BC-Lf) with a high density of alive and metabolically active probiotics on one surface and AgNPs on the opposite one, being probiotics well preserved from the killer effect of AgNPs. The resulting two-sided biomaterial was characterized by Field-Emission Scanning Electron Microscopy (FESEM) and Confocal Laser Scanning Microscopy (CLSM). The antibacterial capacity against Pseudomonas aeruginosa (PA), an opportunistic pathogen responsible for a broad range of skin infections, was also assessed by agar diffusion tests in pathogen-favorable media. Results showed an enhanced activity against PA when both antibacterials were combined into BC (AgNP-BC-Lf) with respect to BC containing only one of the antibacterials, BC-Lf or AgNP-BC. Therefore, AgNP-BC-Lf is an antibiotic-free biomaterial that can be useful for the therapy of topical bacterial infections. |
format | Online Article Text |
id | pubmed-8151946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81519462021-05-27 Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles Sabio, Laura Sosa, Andrea Delgado-López, José M. Dominguez-Vera, José M. Molecules Communication The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two antibacterials, silver nanoparticles (AgNPs) and living probiotics (Lactobacillus fermentum, Lf), using bacterial cellulose (BC) as scaffold. By controlling the loading of each antibacterial at opposite BC sides, we obtained a two-sided biomaterial (AgNP-BC-Lf) with a high density of alive and metabolically active probiotics on one surface and AgNPs on the opposite one, being probiotics well preserved from the killer effect of AgNPs. The resulting two-sided biomaterial was characterized by Field-Emission Scanning Electron Microscopy (FESEM) and Confocal Laser Scanning Microscopy (CLSM). The antibacterial capacity against Pseudomonas aeruginosa (PA), an opportunistic pathogen responsible for a broad range of skin infections, was also assessed by agar diffusion tests in pathogen-favorable media. Results showed an enhanced activity against PA when both antibacterials were combined into BC (AgNP-BC-Lf) with respect to BC containing only one of the antibacterials, BC-Lf or AgNP-BC. Therefore, AgNP-BC-Lf is an antibiotic-free biomaterial that can be useful for the therapy of topical bacterial infections. MDPI 2021-05-11 /pmc/articles/PMC8151946/ /pubmed/34064907 http://dx.doi.org/10.3390/molecules26102848 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Sabio, Laura Sosa, Andrea Delgado-López, José M. Dominguez-Vera, José M. Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles |
title | Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles |
title_full | Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles |
title_fullStr | Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles |
title_full_unstemmed | Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles |
title_short | Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles |
title_sort | two-sided antibacterial cellulose combining probiotics and silver nanoparticles |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151946/ https://www.ncbi.nlm.nih.gov/pubmed/34064907 http://dx.doi.org/10.3390/molecules26102848 |
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