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Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis

Purpose: This prospective, single-center cohort study analyzes the potential of inflammatory protein mediator leucine-rich alpha-2 glycoprotein 1 (LRG1) for the early and accurate diagnosis of acute appendicitis (AA), and differentiation of acute complicated (AcA) from uncomplicated appendicitis (Au...

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Autores principales: Kakar, Mohit, Berezovska, Marisa Maija, Broks, Renars, Asare, Lasma, Delorme, Mathilde, Crouzen, Emile, Zviedre, Astra, Reinis, Aigars, Engelis, Arnis, Kroica, Juta, Saxena, Amulya, Petersons, Aigars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151968/
https://www.ncbi.nlm.nih.gov/pubmed/34064691
http://dx.doi.org/10.3390/diagnostics11050860
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author Kakar, Mohit
Berezovska, Marisa Maija
Broks, Renars
Asare, Lasma
Delorme, Mathilde
Crouzen, Emile
Zviedre, Astra
Reinis, Aigars
Engelis, Arnis
Kroica, Juta
Saxena, Amulya
Petersons, Aigars
author_facet Kakar, Mohit
Berezovska, Marisa Maija
Broks, Renars
Asare, Lasma
Delorme, Mathilde
Crouzen, Emile
Zviedre, Astra
Reinis, Aigars
Engelis, Arnis
Kroica, Juta
Saxena, Amulya
Petersons, Aigars
author_sort Kakar, Mohit
collection PubMed
description Purpose: This prospective, single-center cohort study analyzes the potential of inflammatory protein mediator leucine-rich alpha-2 glycoprotein 1 (LRG1) for the early and accurate diagnosis of acute appendicitis (AA), and differentiation of acute complicated (AcA) from uncomplicated appendicitis (AuA). Methods: Participants were divided into the AcA, AuA, and control groups, and their serum (s-LRG1) and urine LRG1 (u-LRG1) levels were assayed preoperatively on the second and fifth postoperative days. Results: 153 patients participated, 97 had AA. Preoperative u-LRG1 with a cut-off value of 0.18 μg/mL generated an area under the receiver operated characteristic (AUC) curve of 0.70 (95% CI 0.62–0.79) for AA versus control (p < 0.001), while the results for AcA versus AuA were not significant (AUC 0.60, 95% CI 0.49–0.71, p = 0.089). The s-LRG1 levels of AA versus the control with a cut-off value of 51.69 μg/mL generated an AUC of 0.94 (95% CI 0.91–0.99, p < 0.001). The cut-off value of s-LRG1 was 84.06 μg/mL for diagnosis of AcA from AuA, and therefore, significant (AUC 0.69, 95% CI 0.59–0.80, p = 0.001). Conclusions: LRG1 exhibited excellent diagnostic performance as an inexpensive, non-invasive, rapid, and accurate biomarker able to reflect the pathogenesis of AA. LRG1 has the potential to replace advanced imaging to diagnose clinically ambiguous AA cases.
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spelling pubmed-81519682021-05-27 Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis Kakar, Mohit Berezovska, Marisa Maija Broks, Renars Asare, Lasma Delorme, Mathilde Crouzen, Emile Zviedre, Astra Reinis, Aigars Engelis, Arnis Kroica, Juta Saxena, Amulya Petersons, Aigars Diagnostics (Basel) Article Purpose: This prospective, single-center cohort study analyzes the potential of inflammatory protein mediator leucine-rich alpha-2 glycoprotein 1 (LRG1) for the early and accurate diagnosis of acute appendicitis (AA), and differentiation of acute complicated (AcA) from uncomplicated appendicitis (AuA). Methods: Participants were divided into the AcA, AuA, and control groups, and their serum (s-LRG1) and urine LRG1 (u-LRG1) levels were assayed preoperatively on the second and fifth postoperative days. Results: 153 patients participated, 97 had AA. Preoperative u-LRG1 with a cut-off value of 0.18 μg/mL generated an area under the receiver operated characteristic (AUC) curve of 0.70 (95% CI 0.62–0.79) for AA versus control (p < 0.001), while the results for AcA versus AuA were not significant (AUC 0.60, 95% CI 0.49–0.71, p = 0.089). The s-LRG1 levels of AA versus the control with a cut-off value of 51.69 μg/mL generated an AUC of 0.94 (95% CI 0.91–0.99, p < 0.001). The cut-off value of s-LRG1 was 84.06 μg/mL for diagnosis of AcA from AuA, and therefore, significant (AUC 0.69, 95% CI 0.59–0.80, p = 0.001). Conclusions: LRG1 exhibited excellent diagnostic performance as an inexpensive, non-invasive, rapid, and accurate biomarker able to reflect the pathogenesis of AA. LRG1 has the potential to replace advanced imaging to diagnose clinically ambiguous AA cases. MDPI 2021-05-11 /pmc/articles/PMC8151968/ /pubmed/34064691 http://dx.doi.org/10.3390/diagnostics11050860 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kakar, Mohit
Berezovska, Marisa Maija
Broks, Renars
Asare, Lasma
Delorme, Mathilde
Crouzen, Emile
Zviedre, Astra
Reinis, Aigars
Engelis, Arnis
Kroica, Juta
Saxena, Amulya
Petersons, Aigars
Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
title Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
title_full Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
title_fullStr Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
title_full_unstemmed Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
title_short Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
title_sort serum and urine biomarker leucine-rich alpha-2 glycoprotein 1 differentiates pediatric acute complicated and uncomplicated appendicitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151968/
https://www.ncbi.nlm.nih.gov/pubmed/34064691
http://dx.doi.org/10.3390/diagnostics11050860
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