Cargando…

Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test

The prevalence of varicella is especially high among children in the age group of 4–6 years in South Korea, regardless of vaccination. We investigated the immune status of healthy children enrolled in day-care centers and compared pre- and post-vaccination immunity. Antibody titers were measured usi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Yunhwa, Hwang, Ji-Young, Lee, Kyung-Min, Lee, Eunsil, Park, Hosun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151998/
https://www.ncbi.nlm.nih.gov/pubmed/34065863
http://dx.doi.org/10.3390/vaccines9050492
_version_ 1783698516398309376
author Kim, Yunhwa
Hwang, Ji-Young
Lee, Kyung-Min
Lee, Eunsil
Park, Hosun
author_facet Kim, Yunhwa
Hwang, Ji-Young
Lee, Kyung-Min
Lee, Eunsil
Park, Hosun
author_sort Kim, Yunhwa
collection PubMed
description The prevalence of varicella is especially high among children in the age group of 4–6 years in South Korea, regardless of vaccination. We investigated the immune status of healthy children enrolled in day-care centers and compared pre- and post-vaccination immunity. Antibody titers were measured using a glycoprotein enzyme-linked immunosorbent assay (gpEIA) kit, and the seroconversion rate was assessed using a fluorescent antibody to membrane antigen (FAMA) test. Among 541 vaccinated children, 109 (20.1%) had breakthrough varicella. However, 13 (72.2%) of the 18 unvaccinated children had a history of varicella. The gpEIA geometric mean titers (GMTs) of pre- and 5 weeks post-vaccination in 1-year-old children were 14.7 and 72 mIU/mL, respectively, and the FAMA seroconversion rate was 91.1%. The gpEIA GMTs of 2-, 3-, 4-, 5-, and 6-year-old children were 104.1, 133.8, 223.5, 364.1, and 353.0 mIU/mL, respectively. Even though the gpEIA GMT increased with age, the pattern of gpEIA titer distribution in 4- to 6-year-old vaccinees without varicella history represented both waning immunity and natural boosting immunity. These results suggest that some vaccinees are vulnerable to varicella infection. Therefore, it is necessary to consider a two-dose varicella vaccine regimen in South Korea.
format Online
Article
Text
id pubmed-8151998
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81519982021-05-27 Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test Kim, Yunhwa Hwang, Ji-Young Lee, Kyung-Min Lee, Eunsil Park, Hosun Vaccines (Basel) Article The prevalence of varicella is especially high among children in the age group of 4–6 years in South Korea, regardless of vaccination. We investigated the immune status of healthy children enrolled in day-care centers and compared pre- and post-vaccination immunity. Antibody titers were measured using a glycoprotein enzyme-linked immunosorbent assay (gpEIA) kit, and the seroconversion rate was assessed using a fluorescent antibody to membrane antigen (FAMA) test. Among 541 vaccinated children, 109 (20.1%) had breakthrough varicella. However, 13 (72.2%) of the 18 unvaccinated children had a history of varicella. The gpEIA geometric mean titers (GMTs) of pre- and 5 weeks post-vaccination in 1-year-old children were 14.7 and 72 mIU/mL, respectively, and the FAMA seroconversion rate was 91.1%. The gpEIA GMTs of 2-, 3-, 4-, 5-, and 6-year-old children were 104.1, 133.8, 223.5, 364.1, and 353.0 mIU/mL, respectively. Even though the gpEIA GMT increased with age, the pattern of gpEIA titer distribution in 4- to 6-year-old vaccinees without varicella history represented both waning immunity and natural boosting immunity. These results suggest that some vaccinees are vulnerable to varicella infection. Therefore, it is necessary to consider a two-dose varicella vaccine regimen in South Korea. MDPI 2021-05-12 /pmc/articles/PMC8151998/ /pubmed/34065863 http://dx.doi.org/10.3390/vaccines9050492 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yunhwa
Hwang, Ji-Young
Lee, Kyung-Min
Lee, Eunsil
Park, Hosun
Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test
title Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test
title_full Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test
title_fullStr Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test
title_full_unstemmed Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test
title_short Cross-Sectional Study of Varicella Zoster Virus Immunity in Healthy Korean Children Assessed by Glycoprotein Enzyme-Linked Immunosorbent Assay and Fluorescent Antibody to Membrane Antigen Test
title_sort cross-sectional study of varicella zoster virus immunity in healthy korean children assessed by glycoprotein enzyme-linked immunosorbent assay and fluorescent antibody to membrane antigen test
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151998/
https://www.ncbi.nlm.nih.gov/pubmed/34065863
http://dx.doi.org/10.3390/vaccines9050492
work_keys_str_mv AT kimyunhwa crosssectionalstudyofvaricellazostervirusimmunityinhealthykoreanchildrenassessedbyglycoproteinenzymelinkedimmunosorbentassayandfluorescentantibodytomembraneantigentest
AT hwangjiyoung crosssectionalstudyofvaricellazostervirusimmunityinhealthykoreanchildrenassessedbyglycoproteinenzymelinkedimmunosorbentassayandfluorescentantibodytomembraneantigentest
AT leekyungmin crosssectionalstudyofvaricellazostervirusimmunityinhealthykoreanchildrenassessedbyglycoproteinenzymelinkedimmunosorbentassayandfluorescentantibodytomembraneantigentest
AT leeeunsil crosssectionalstudyofvaricellazostervirusimmunityinhealthykoreanchildrenassessedbyglycoproteinenzymelinkedimmunosorbentassayandfluorescentantibodytomembraneantigentest
AT parkhosun crosssectionalstudyofvaricellazostervirusimmunityinhealthykoreanchildrenassessedbyglycoproteinenzymelinkedimmunosorbentassayandfluorescentantibodytomembraneantigentest