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Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae

Microalgal triacylglycerols (TAGs) are a good feedstock for liquid biofuel production. Improving the expression and/or function of transcription factors (TFs) involved in TAG accumulation may increase TAG content; however, information on microalgae is still lacking. In this study, 14 TFs in the unic...

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Autores principales: Takahashi, Sota, Okubo, Riho, Kanesaki, Yu, Zhou, Baifeng, Takaya, Kazuhiro, Watanabe, Satoru, Tanaka, Kan, Imamura, Sousuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152781/
https://www.ncbi.nlm.nih.gov/pubmed/34068121
http://dx.doi.org/10.3390/plants10050971
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author Takahashi, Sota
Okubo, Riho
Kanesaki, Yu
Zhou, Baifeng
Takaya, Kazuhiro
Watanabe, Satoru
Tanaka, Kan
Imamura, Sousuke
author_facet Takahashi, Sota
Okubo, Riho
Kanesaki, Yu
Zhou, Baifeng
Takaya, Kazuhiro
Watanabe, Satoru
Tanaka, Kan
Imamura, Sousuke
author_sort Takahashi, Sota
collection PubMed
description Microalgal triacylglycerols (TAGs) are a good feedstock for liquid biofuel production. Improving the expression and/or function of transcription factors (TFs) involved in TAG accumulation may increase TAG content; however, information on microalgae is still lacking. In this study, 14 TFs in the unicellular red alga Cyanidioschyzon merolae were identified as candidate TFs regulating TAG accumulation using available transcriptome and phosphoproteome data under conditions driving TAG accumulation. To investigate the roles of these TFs, we constructed TF-overexpression strains and analyzed lipid droplet (LD) formation and TAG contents in the cells grown under standard conditions. Based on the results, we identified four TFs involved in LD and TAG accumulation. RNA-Seq analyses were performed to identify genes regulated by the four TFs using each overexpression strain. Among the TAG biosynthesis-related genes, only the gene encoding the endoplasmic reticulum-localized lysophosphatidic acid acyltransferase 1 (LPAT1) was notably increased among the overexpression strains. In the LPAT1 overexpression strain, TAG accumulation was significantly increased compared with the control strain under normal growth conditions. These results indicate that the four TFs positively regulate TAG accumulation by changing their target gene expression in C. merolae.
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spelling pubmed-81527812021-05-27 Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae Takahashi, Sota Okubo, Riho Kanesaki, Yu Zhou, Baifeng Takaya, Kazuhiro Watanabe, Satoru Tanaka, Kan Imamura, Sousuke Plants (Basel) Article Microalgal triacylglycerols (TAGs) are a good feedstock for liquid biofuel production. Improving the expression and/or function of transcription factors (TFs) involved in TAG accumulation may increase TAG content; however, information on microalgae is still lacking. In this study, 14 TFs in the unicellular red alga Cyanidioschyzon merolae were identified as candidate TFs regulating TAG accumulation using available transcriptome and phosphoproteome data under conditions driving TAG accumulation. To investigate the roles of these TFs, we constructed TF-overexpression strains and analyzed lipid droplet (LD) formation and TAG contents in the cells grown under standard conditions. Based on the results, we identified four TFs involved in LD and TAG accumulation. RNA-Seq analyses were performed to identify genes regulated by the four TFs using each overexpression strain. Among the TAG biosynthesis-related genes, only the gene encoding the endoplasmic reticulum-localized lysophosphatidic acid acyltransferase 1 (LPAT1) was notably increased among the overexpression strains. In the LPAT1 overexpression strain, TAG accumulation was significantly increased compared with the control strain under normal growth conditions. These results indicate that the four TFs positively regulate TAG accumulation by changing their target gene expression in C. merolae. MDPI 2021-05-13 /pmc/articles/PMC8152781/ /pubmed/34068121 http://dx.doi.org/10.3390/plants10050971 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takahashi, Sota
Okubo, Riho
Kanesaki, Yu
Zhou, Baifeng
Takaya, Kazuhiro
Watanabe, Satoru
Tanaka, Kan
Imamura, Sousuke
Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
title Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
title_full Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
title_fullStr Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
title_full_unstemmed Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
title_short Identification of Transcription Factors and the Regulatory Genes Involved in Triacylglycerol Accumulation in the Unicellular Red Alga Cyanidioschyzon merolae
title_sort identification of transcription factors and the regulatory genes involved in triacylglycerol accumulation in the unicellular red alga cyanidioschyzon merolae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152781/
https://www.ncbi.nlm.nih.gov/pubmed/34068121
http://dx.doi.org/10.3390/plants10050971
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