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Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?

Breeding programs in ornamentals can be facilitated by integrating knowledge of phylogenetic relatedness of potential parents along with other genomic information. Using AFLP, genetic distances were determined for 59 Geranium genotypes, comprising 55 commercial cultivars of the three subgenera of a...

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Autores principales: Akbarzadeh, Mehrdad, Van Laere, Katrijn, Leus, Leen, De Riek, Jan, Van Huylenbroeck, Johan, Werbrouck, Stefaan P.O., Dhooghe, Emmy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152959/
https://www.ncbi.nlm.nih.gov/pubmed/34068148
http://dx.doi.org/10.3390/genes12050730
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author Akbarzadeh, Mehrdad
Van Laere, Katrijn
Leus, Leen
De Riek, Jan
Van Huylenbroeck, Johan
Werbrouck, Stefaan P.O.
Dhooghe, Emmy
author_facet Akbarzadeh, Mehrdad
Van Laere, Katrijn
Leus, Leen
De Riek, Jan
Van Huylenbroeck, Johan
Werbrouck, Stefaan P.O.
Dhooghe, Emmy
author_sort Akbarzadeh, Mehrdad
collection PubMed
description Breeding programs in ornamentals can be facilitated by integrating knowledge of phylogenetic relatedness of potential parents along with other genomic information. Using AFLP, genetic distances were determined for 59 Geranium genotypes, comprising 55 commercial cultivars of the three subgenera of a total collection of 61 Geranium genotypes. A subgroup of 45 genotypes, including intragroup and intergroup hybrids, were selected and further characterized for genome sizes and chromosome numbers. The variation in genome size ranged from 1.51 ± 0.01 pg/2C to 12.94 ± 0.07 pg/2C. The chromosome numbers ranged from 26 to 108–110 with some hybrids showing an aberrant number of chromosomes based on their parents’ constitution. All chromosome numbers of Geranium are an even number, which presumes that unreduced gametes occur in some cross combinations. Overall, parental difference in genome size and chromosome number were not limiting for cross compatibility. Good crossing compatibility was correlated to a Jaccard similarity coefficient as parameter for parental relatedness of about 0.5. Additionally, parent combinations with high differences in the DNA/chromosome value could not result in a successful cross. We expect that our results will enable breeding programs to overcome crossing barriers and support further breeding initiatives.
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spelling pubmed-81529592021-05-27 Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums? Akbarzadeh, Mehrdad Van Laere, Katrijn Leus, Leen De Riek, Jan Van Huylenbroeck, Johan Werbrouck, Stefaan P.O. Dhooghe, Emmy Genes (Basel) Article Breeding programs in ornamentals can be facilitated by integrating knowledge of phylogenetic relatedness of potential parents along with other genomic information. Using AFLP, genetic distances were determined for 59 Geranium genotypes, comprising 55 commercial cultivars of the three subgenera of a total collection of 61 Geranium genotypes. A subgroup of 45 genotypes, including intragroup and intergroup hybrids, were selected and further characterized for genome sizes and chromosome numbers. The variation in genome size ranged from 1.51 ± 0.01 pg/2C to 12.94 ± 0.07 pg/2C. The chromosome numbers ranged from 26 to 108–110 with some hybrids showing an aberrant number of chromosomes based on their parents’ constitution. All chromosome numbers of Geranium are an even number, which presumes that unreduced gametes occur in some cross combinations. Overall, parental difference in genome size and chromosome number were not limiting for cross compatibility. Good crossing compatibility was correlated to a Jaccard similarity coefficient as parameter for parental relatedness of about 0.5. Additionally, parent combinations with high differences in the DNA/chromosome value could not result in a successful cross. We expect that our results will enable breeding programs to overcome crossing barriers and support further breeding initiatives. MDPI 2021-05-13 /pmc/articles/PMC8152959/ /pubmed/34068148 http://dx.doi.org/10.3390/genes12050730 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akbarzadeh, Mehrdad
Van Laere, Katrijn
Leus, Leen
De Riek, Jan
Van Huylenbroeck, Johan
Werbrouck, Stefaan P.O.
Dhooghe, Emmy
Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
title Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
title_full Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
title_fullStr Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
title_full_unstemmed Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
title_short Can Knowledge of Genetic Distances, Genome Sizes and Chromosome Numbers Support Breeding Programs in Hardy Geraniums?
title_sort can knowledge of genetic distances, genome sizes and chromosome numbers support breeding programs in hardy geraniums?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152959/
https://www.ncbi.nlm.nih.gov/pubmed/34068148
http://dx.doi.org/10.3390/genes12050730
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