Intestinal Microbes of Hooded Cranes (Grus monacha) Wintering in Three Lakes of the Middle and Lower Yangtze River Floodplain

SIMPLE SUMMARY: Intestinal microbes are critical to host health, and are affected by environmental factors. In this study, we investigated the intestinal microbes of Hooded Cranes wintering at three lakes with different environmental characteristics in the middle and lower Yangtze River floodplain in China, aiming to provide insights into the effects of habitat size and protection status of birds on their intestinal microbes. We found that the Hooded Cranes at the smaller lake had higher intestinal bacterial and fungal diversity than those at the larger lake. In addition, more diverse and abundant pathogens were found in the gut of Hooded Cranes that lived in the relatively poorly protected habitat than those that lived in well-protected habitat. This study contributes a new perspective for understanding the intestinal microbes of wintering migratory waterbirds at different habitats, and will help to understand the survival status of the vulnerable waterbirds at different habitats for their better conservation. ABSTRACT: Intestinal microbes participate in life activities of the host, and are affected by external environmental factors. Different habitat sizes and protection status provide different external environmental selection pressures for the same wintering waterbirds, which may be reflected in their intestinal microbes. Hooded Cranes are vulnerable migratory waterbirds with similar numbers wintering at three different lakes in the middle and lower Yangtze River floodplain, Poyang, Caizi, and Shengjin Lakes. Here, we analyzed the characteristics of intestinal bacterial and fungal communities of Hooded Cranes wintering at the three lakes to clarify the effect of habitat size and protection status on intestinal microbes, using high-throughput sequencing technology. Our results showed that community composition and diversity of intestinal microbes were significantly different among lakes with different habitat size and protection status. The Hooded Cranes at Shengjin Lake (small) had higher intestinal microbial alpha-diversity (for both bacteria and fungi) than those at Poyang Lake (large), which might be induced by social behavior of more waterbirds per unit area. The Hooded Cranes at Caizi Lake (relatively poorly protected habitat) had more diverse and abundant intestinal potential pathogens than Shengjin Lake (well-protected habitat). Our results indicated that the environmental pressure of a habitat might affect intestinal microorganisms and more attention might be needed for the vulnerable waterbirds at the habitat of poor protection status.