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Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol

Emerging evidence supports a significant association between human cytomegalovirus (HCMV) and human malignancies, suggesting HCMV as a human oncomodulatory virus. HCMV gene products are found in >90% of breast cancer tumors and seem to be correlated with more aggressive disease. The definitive di...

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Autores principales: Touma, Joel, Liu, Yan, Rahbar, Afsar, Pantalone, Mattia Russel, Almazan, Nerea Martin, Vetvik, Katja, Söderberg-Nauclér, Cecilia, Geisler, Jürgen, Sauer, Torill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153275/
https://www.ncbi.nlm.nih.gov/pubmed/34068349
http://dx.doi.org/10.3390/microorganisms9051059
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author Touma, Joel
Liu, Yan
Rahbar, Afsar
Pantalone, Mattia Russel
Almazan, Nerea Martin
Vetvik, Katja
Söderberg-Nauclér, Cecilia
Geisler, Jürgen
Sauer, Torill
author_facet Touma, Joel
Liu, Yan
Rahbar, Afsar
Pantalone, Mattia Russel
Almazan, Nerea Martin
Vetvik, Katja
Söderberg-Nauclér, Cecilia
Geisler, Jürgen
Sauer, Torill
author_sort Touma, Joel
collection PubMed
description Emerging evidence supports a significant association between human cytomegalovirus (HCMV) and human malignancies, suggesting HCMV as a human oncomodulatory virus. HCMV gene products are found in >90% of breast cancer tumors and seem to be correlated with more aggressive disease. The definitive diagnosis of HCMV relies on identification of virus inclusions and/or viral proteins by different techniques including immunohistochemical staining. In order to reduce biases and improve clinical value of HCMV diagnostics in oncological pathology, automation of the procedure is needed and this was the purpose of this study. Tumor specimens from 115 patients treated for primary breast cancer at Akershus University Hospital in Norway were available for the validation of the staining method in this retrospective study. We demonstrate that our method is highly sensitive and delivers excellent reproducibility for staining of HCMV late antigen (LA), which makes this method useful for future routine diagnostics and scientific applications.
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spelling pubmed-81532752021-05-27 Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol Touma, Joel Liu, Yan Rahbar, Afsar Pantalone, Mattia Russel Almazan, Nerea Martin Vetvik, Katja Söderberg-Nauclér, Cecilia Geisler, Jürgen Sauer, Torill Microorganisms Article Emerging evidence supports a significant association between human cytomegalovirus (HCMV) and human malignancies, suggesting HCMV as a human oncomodulatory virus. HCMV gene products are found in >90% of breast cancer tumors and seem to be correlated with more aggressive disease. The definitive diagnosis of HCMV relies on identification of virus inclusions and/or viral proteins by different techniques including immunohistochemical staining. In order to reduce biases and improve clinical value of HCMV diagnostics in oncological pathology, automation of the procedure is needed and this was the purpose of this study. Tumor specimens from 115 patients treated for primary breast cancer at Akershus University Hospital in Norway were available for the validation of the staining method in this retrospective study. We demonstrate that our method is highly sensitive and delivers excellent reproducibility for staining of HCMV late antigen (LA), which makes this method useful for future routine diagnostics and scientific applications. MDPI 2021-05-13 /pmc/articles/PMC8153275/ /pubmed/34068349 http://dx.doi.org/10.3390/microorganisms9051059 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Touma, Joel
Liu, Yan
Rahbar, Afsar
Pantalone, Mattia Russel
Almazan, Nerea Martin
Vetvik, Katja
Söderberg-Nauclér, Cecilia
Geisler, Jürgen
Sauer, Torill
Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol
title Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol
title_full Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol
title_fullStr Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol
title_full_unstemmed Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol
title_short Detection of Human Cytomegalovirus Proteins in Paraffin-Embedded Breast Cancer Tissue Specimens—A Novel, Automated Immunohistochemical Staining Protocol
title_sort detection of human cytomegalovirus proteins in paraffin-embedded breast cancer tissue specimens—a novel, automated immunohistochemical staining protocol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153275/
https://www.ncbi.nlm.nih.gov/pubmed/34068349
http://dx.doi.org/10.3390/microorganisms9051059
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