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Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures

Rapid determination of bacterial antibiotic susceptibility is important for proper treatment of infections. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) has recently published guidelines for rapid antimicrobial susceptibility testing (RAST) performed directly from positive...

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Autores principales: Shifman, Ohad, Aminov, Tamar, Aftalion, Moshe, Gur, David, Cohen, Hila, Bar-David, Elad, Cohen, Ofer, Mamroud, Emanuelle, Levy, Haim, Aloni-Grinstein, Ronit, Steinberger-Levy, Ida, Rotem, Shahar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153291/
https://www.ncbi.nlm.nih.gov/pubmed/34068310
http://dx.doi.org/10.3390/microorganisms9051055
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author Shifman, Ohad
Aminov, Tamar
Aftalion, Moshe
Gur, David
Cohen, Hila
Bar-David, Elad
Cohen, Ofer
Mamroud, Emanuelle
Levy, Haim
Aloni-Grinstein, Ronit
Steinberger-Levy, Ida
Rotem, Shahar
author_facet Shifman, Ohad
Aminov, Tamar
Aftalion, Moshe
Gur, David
Cohen, Hila
Bar-David, Elad
Cohen, Ofer
Mamroud, Emanuelle
Levy, Haim
Aloni-Grinstein, Ronit
Steinberger-Levy, Ida
Rotem, Shahar
author_sort Shifman, Ohad
collection PubMed
description Rapid determination of bacterial antibiotic susceptibility is important for proper treatment of infections. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) has recently published guidelines for rapid antimicrobial susceptibility testing (RAST) performed directly from positive blood culture vials. These guidelines, however, were only published for a limited number of common pathogenic bacteria. In this study, we evaluated the applicability of these guidelines to three Tier 1 bioterror agents (Bacillus anthracis, Yersinia pestis and Francisella tularensis) that require prompt antibiotic treatment to mitigate morbidity and mortality. We used spiked-in human blood incubated in a BACTEC™ FX40 system to determine the proper conditions for RAST using disc-diffusion and Etest assays. We found that reliable disc-diffusion inhibition diameters and Etest MIC values could be obtained in remarkably short times. Compared to the EUCAST-recommended disc-diffusion assays that will require adjusted clinical breakpoint tables, Etest-based RAST was advantageous, as the obtained MIC values were similar to the standard MIC values, enabling the use of established category breakpoint tables. Our results demonstrate the promising applicability of the EUCAST RAST for B. anthracis-, Y. pestis- or F. tularensis-positive blood cultures, which can lead to shorter diagnostics and prompt antibiotic treatment of these dangerous pathogens.
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spelling pubmed-81532912021-05-27 Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures Shifman, Ohad Aminov, Tamar Aftalion, Moshe Gur, David Cohen, Hila Bar-David, Elad Cohen, Ofer Mamroud, Emanuelle Levy, Haim Aloni-Grinstein, Ronit Steinberger-Levy, Ida Rotem, Shahar Microorganisms Article Rapid determination of bacterial antibiotic susceptibility is important for proper treatment of infections. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) has recently published guidelines for rapid antimicrobial susceptibility testing (RAST) performed directly from positive blood culture vials. These guidelines, however, were only published for a limited number of common pathogenic bacteria. In this study, we evaluated the applicability of these guidelines to three Tier 1 bioterror agents (Bacillus anthracis, Yersinia pestis and Francisella tularensis) that require prompt antibiotic treatment to mitigate morbidity and mortality. We used spiked-in human blood incubated in a BACTEC™ FX40 system to determine the proper conditions for RAST using disc-diffusion and Etest assays. We found that reliable disc-diffusion inhibition diameters and Etest MIC values could be obtained in remarkably short times. Compared to the EUCAST-recommended disc-diffusion assays that will require adjusted clinical breakpoint tables, Etest-based RAST was advantageous, as the obtained MIC values were similar to the standard MIC values, enabling the use of established category breakpoint tables. Our results demonstrate the promising applicability of the EUCAST RAST for B. anthracis-, Y. pestis- or F. tularensis-positive blood cultures, which can lead to shorter diagnostics and prompt antibiotic treatment of these dangerous pathogens. MDPI 2021-05-13 /pmc/articles/PMC8153291/ /pubmed/34068310 http://dx.doi.org/10.3390/microorganisms9051055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shifman, Ohad
Aminov, Tamar
Aftalion, Moshe
Gur, David
Cohen, Hila
Bar-David, Elad
Cohen, Ofer
Mamroud, Emanuelle
Levy, Haim
Aloni-Grinstein, Ronit
Steinberger-Levy, Ida
Rotem, Shahar
Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures
title Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures
title_full Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures
title_fullStr Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures
title_full_unstemmed Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures
title_short Evaluation of the European Committee on Antimicrobial Susceptibility Testing Guidelines for Rapid Antimicrobial Susceptibility Testing of Bacillus anthracis-, Yersinia pestis- and Francisella tularensis-Positive Blood Cultures
title_sort evaluation of the european committee on antimicrobial susceptibility testing guidelines for rapid antimicrobial susceptibility testing of bacillus anthracis-, yersinia pestis- and francisella tularensis-positive blood cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153291/
https://www.ncbi.nlm.nih.gov/pubmed/34068310
http://dx.doi.org/10.3390/microorganisms9051055
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