Cargando…

Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells

Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as st...

Descripción completa

Detalles Bibliográficos
Autores principales: Garzón, Ingrid, Jaimes-Parra, Boris Damián, Pascual-Geler, Manrique, Cózar, José Manuel, Sánchez-Quevedo, María del Carmen, Mosquera-Pacheco, María Auxiliadora, Sánchez-Montesinos, Indalecio, Fernández-Valadés, Ricardo, Campos, Fernando, Alaminos, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153323/
https://www.ncbi.nlm.nih.gov/pubmed/34068343
http://dx.doi.org/10.3390/polym13101568
_version_ 1783698775612588032
author Garzón, Ingrid
Jaimes-Parra, Boris Damián
Pascual-Geler, Manrique
Cózar, José Manuel
Sánchez-Quevedo, María del Carmen
Mosquera-Pacheco, María Auxiliadora
Sánchez-Montesinos, Indalecio
Fernández-Valadés, Ricardo
Campos, Fernando
Alaminos, Miguel
author_facet Garzón, Ingrid
Jaimes-Parra, Boris Damián
Pascual-Geler, Manrique
Cózar, José Manuel
Sánchez-Quevedo, María del Carmen
Mosquera-Pacheco, María Auxiliadora
Sánchez-Montesinos, Indalecio
Fernández-Valadés, Ricardo
Campos, Fernando
Alaminos, Miguel
author_sort Garzón, Ingrid
collection PubMed
description Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use.
format Online
Article
Text
id pubmed-8153323
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81533232021-05-27 Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells Garzón, Ingrid Jaimes-Parra, Boris Damián Pascual-Geler, Manrique Cózar, José Manuel Sánchez-Quevedo, María del Carmen Mosquera-Pacheco, María Auxiliadora Sánchez-Montesinos, Indalecio Fernández-Valadés, Ricardo Campos, Fernando Alaminos, Miguel Polymers (Basel) Article Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use. MDPI 2021-05-13 /pmc/articles/PMC8153323/ /pubmed/34068343 http://dx.doi.org/10.3390/polym13101568 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garzón, Ingrid
Jaimes-Parra, Boris Damián
Pascual-Geler, Manrique
Cózar, José Manuel
Sánchez-Quevedo, María del Carmen
Mosquera-Pacheco, María Auxiliadora
Sánchez-Montesinos, Indalecio
Fernández-Valadés, Ricardo
Campos, Fernando
Alaminos, Miguel
Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
title Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
title_full Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
title_fullStr Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
title_full_unstemmed Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
title_short Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
title_sort biofabrication of a tubular model of human urothelial mucosa using human wharton jelly mesenchymal stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153323/
https://www.ncbi.nlm.nih.gov/pubmed/34068343
http://dx.doi.org/10.3390/polym13101568
work_keys_str_mv AT garzoningrid biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT jaimesparraborisdamian biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT pascualgelermanrique biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT cozarjosemanuel biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT sanchezquevedomariadelcarmen biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT mosquerapachecomariaauxiliadora biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT sanchezmontesinosindalecio biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT fernandezvaladesricardo biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT camposfernando biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells
AT alaminosmiguel biofabricationofatubularmodelofhumanurothelialmucosausinghumanwhartonjellymesenchymalstromalcells