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Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as st...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153323/ https://www.ncbi.nlm.nih.gov/pubmed/34068343 http://dx.doi.org/10.3390/polym13101568 |
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author | Garzón, Ingrid Jaimes-Parra, Boris Damián Pascual-Geler, Manrique Cózar, José Manuel Sánchez-Quevedo, María del Carmen Mosquera-Pacheco, María Auxiliadora Sánchez-Montesinos, Indalecio Fernández-Valadés, Ricardo Campos, Fernando Alaminos, Miguel |
author_facet | Garzón, Ingrid Jaimes-Parra, Boris Damián Pascual-Geler, Manrique Cózar, José Manuel Sánchez-Quevedo, María del Carmen Mosquera-Pacheco, María Auxiliadora Sánchez-Montesinos, Indalecio Fernández-Valadés, Ricardo Campos, Fernando Alaminos, Miguel |
author_sort | Garzón, Ingrid |
collection | PubMed |
description | Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use. |
format | Online Article Text |
id | pubmed-8153323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81533232021-05-27 Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells Garzón, Ingrid Jaimes-Parra, Boris Damián Pascual-Geler, Manrique Cózar, José Manuel Sánchez-Quevedo, María del Carmen Mosquera-Pacheco, María Auxiliadora Sánchez-Montesinos, Indalecio Fernández-Valadés, Ricardo Campos, Fernando Alaminos, Miguel Polymers (Basel) Article Several models of bioartificial human urothelial mucosa (UM) have been described recently. In this study, we generated novel tubularized UM substitutes using alternative sources of cells. Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC) were used to generate an epithelial-like layer on top. Three differentiation media were used for 7 and 14 days. Results showed that the biofabrication methods used here succeeded in generating a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top, especially using a medium containing epithelial growth and differentiation factors (EM), although differentiation was not complete. At the functional level, UM substitutes were able to synthesize collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tight-junction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods explored here were able to generate a potential substitute of the human UM for future clinical use. MDPI 2021-05-13 /pmc/articles/PMC8153323/ /pubmed/34068343 http://dx.doi.org/10.3390/polym13101568 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Garzón, Ingrid Jaimes-Parra, Boris Damián Pascual-Geler, Manrique Cózar, José Manuel Sánchez-Quevedo, María del Carmen Mosquera-Pacheco, María Auxiliadora Sánchez-Montesinos, Indalecio Fernández-Valadés, Ricardo Campos, Fernando Alaminos, Miguel Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells |
title | Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells |
title_full | Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells |
title_fullStr | Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells |
title_full_unstemmed | Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells |
title_short | Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells |
title_sort | biofabrication of a tubular model of human urothelial mucosa using human wharton jelly mesenchymal stromal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153323/ https://www.ncbi.nlm.nih.gov/pubmed/34068343 http://dx.doi.org/10.3390/polym13101568 |
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