Cargando…
RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sa...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153481/ https://www.ncbi.nlm.nih.gov/pubmed/34038467 http://dx.doi.org/10.1371/journal.pone.0252022 |
_version_ | 1783698808255807488 |
---|---|
author | Barsøe, Sofie Ullman, Karin Leijon, Mikael Hedlund, Kjell Olof Klingström, Jonas Krarup, Louise Iuel Andresen, Lars Quaade, Michelle Lauge Hammer, Anne Sofie |
author_facet | Barsøe, Sofie Ullman, Karin Leijon, Mikael Hedlund, Kjell Olof Klingström, Jonas Krarup, Louise Iuel Andresen, Lars Quaade, Michelle Lauge Hammer, Anne Sofie |
author_sort | Barsøe, Sofie |
collection | PubMed |
description | Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals were classified based on age and patoanatomical evaluation as having pre-weaning diarrhea, diarrhea in the growth period or as having no macroscopic signs of diarrhea. Fecal samples were analyzed for MiAstV with RT-qPCR, next generation sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by next generation sequencing in a high proportion of samples (22/27), and by visualization of astrovirus particles with EM in some of the samples. Mink astrovirus was highly prevalent (68%) among kits in the outbreaks of pre-weaning diarrhea, in particular outbreaks from May, while less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea in the growth period, though in a much lesser extent than in the pre-weaning period. The role of mink astrovirus in the diarrhea disease complex of mink remain to be investigated, and for that purpose this sensitive and robust RT-qPCR can be a valuable tool in the future. |
format | Online Article Text |
id | pubmed-8153481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81534812021-06-09 RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms Barsøe, Sofie Ullman, Karin Leijon, Mikael Hedlund, Kjell Olof Klingström, Jonas Krarup, Louise Iuel Andresen, Lars Quaade, Michelle Lauge Hammer, Anne Sofie PLoS One Research Article Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals were classified based on age and patoanatomical evaluation as having pre-weaning diarrhea, diarrhea in the growth period or as having no macroscopic signs of diarrhea. Fecal samples were analyzed for MiAstV with RT-qPCR, next generation sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by next generation sequencing in a high proportion of samples (22/27), and by visualization of astrovirus particles with EM in some of the samples. Mink astrovirus was highly prevalent (68%) among kits in the outbreaks of pre-weaning diarrhea, in particular outbreaks from May, while less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea in the growth period, though in a much lesser extent than in the pre-weaning period. The role of mink astrovirus in the diarrhea disease complex of mink remain to be investigated, and for that purpose this sensitive and robust RT-qPCR can be a valuable tool in the future. Public Library of Science 2021-05-26 /pmc/articles/PMC8153481/ /pubmed/34038467 http://dx.doi.org/10.1371/journal.pone.0252022 Text en © 2021 Barsøe et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Barsøe, Sofie Ullman, Karin Leijon, Mikael Hedlund, Kjell Olof Klingström, Jonas Krarup, Louise Iuel Andresen, Lars Quaade, Michelle Lauge Hammer, Anne Sofie RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms |
title | RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms |
title_full | RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms |
title_fullStr | RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms |
title_full_unstemmed | RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms |
title_short | RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms |
title_sort | rt-qpcr assay for detection of mink astrovirus in outbreaks of diarrhea on danish mink farms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153481/ https://www.ncbi.nlm.nih.gov/pubmed/34038467 http://dx.doi.org/10.1371/journal.pone.0252022 |
work_keys_str_mv | AT barsøesofie rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT ullmankarin rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT leijonmikael rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT hedlundkjellolof rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT klingstromjonas rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT kraruplouiseiuel rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT andresenlars rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT quaademichellelauge rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms AT hammerannesofie rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms |