Cargando…

RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms

Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sa...

Descripción completa

Detalles Bibliográficos
Autores principales: Barsøe, Sofie, Ullman, Karin, Leijon, Mikael, Hedlund, Kjell Olof, Klingström, Jonas, Krarup, Louise Iuel, Andresen, Lars, Quaade, Michelle Lauge, Hammer, Anne Sofie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153481/
https://www.ncbi.nlm.nih.gov/pubmed/34038467
http://dx.doi.org/10.1371/journal.pone.0252022
_version_ 1783698808255807488
author Barsøe, Sofie
Ullman, Karin
Leijon, Mikael
Hedlund, Kjell Olof
Klingström, Jonas
Krarup, Louise Iuel
Andresen, Lars
Quaade, Michelle Lauge
Hammer, Anne Sofie
author_facet Barsøe, Sofie
Ullman, Karin
Leijon, Mikael
Hedlund, Kjell Olof
Klingström, Jonas
Krarup, Louise Iuel
Andresen, Lars
Quaade, Michelle Lauge
Hammer, Anne Sofie
author_sort Barsøe, Sofie
collection PubMed
description Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals were classified based on age and patoanatomical evaluation as having pre-weaning diarrhea, diarrhea in the growth period or as having no macroscopic signs of diarrhea. Fecal samples were analyzed for MiAstV with RT-qPCR, next generation sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by next generation sequencing in a high proportion of samples (22/27), and by visualization of astrovirus particles with EM in some of the samples. Mink astrovirus was highly prevalent (68%) among kits in the outbreaks of pre-weaning diarrhea, in particular outbreaks from May, while less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea in the growth period, though in a much lesser extent than in the pre-weaning period. The role of mink astrovirus in the diarrhea disease complex of mink remain to be investigated, and for that purpose this sensitive and robust RT-qPCR can be a valuable tool in the future.
format Online
Article
Text
id pubmed-8153481
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-81534812021-06-09 RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms Barsøe, Sofie Ullman, Karin Leijon, Mikael Hedlund, Kjell Olof Klingström, Jonas Krarup, Louise Iuel Andresen, Lars Quaade, Michelle Lauge Hammer, Anne Sofie PLoS One Research Article Diarrhea in mink kits is a major cause of disease and mortality in the mink production. The etiology remains unknown in most outbreaks due to a lack of diagnostic assays. In the current study we present an RT-qPCR method to detect mink astrovirus in fecal samples from mink kits with diarrhea. All sampled animals were classified based on age and patoanatomical evaluation as having pre-weaning diarrhea, diarrhea in the growth period or as having no macroscopic signs of diarrhea. Fecal samples were analyzed for MiAstV with RT-qPCR, next generation sequencing and electron microscopy in parallel. Mink astrovirus was detected with RT-qPCR in 92 out of 203 samples. This detection was confirmed by next generation sequencing in a high proportion of samples (22/27), and by visualization of astrovirus particles with EM in some of the samples. Mink astrovirus was highly prevalent (68%) among kits in the outbreaks of pre-weaning diarrhea, in particular outbreaks from May, while less prevalent in outbreaks in June. Mink astrovirus was detected in outbreaks of diarrhea in the growth period, though in a much lesser extent than in the pre-weaning period. The role of mink astrovirus in the diarrhea disease complex of mink remain to be investigated, and for that purpose this sensitive and robust RT-qPCR can be a valuable tool in the future. Public Library of Science 2021-05-26 /pmc/articles/PMC8153481/ /pubmed/34038467 http://dx.doi.org/10.1371/journal.pone.0252022 Text en © 2021 Barsøe et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barsøe, Sofie
Ullman, Karin
Leijon, Mikael
Hedlund, Kjell Olof
Klingström, Jonas
Krarup, Louise Iuel
Andresen, Lars
Quaade, Michelle Lauge
Hammer, Anne Sofie
RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
title RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
title_full RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
title_fullStr RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
title_full_unstemmed RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
title_short RT-qPCR assay for detection of mink astrovirus in outbreaks of diarrhea on Danish mink farms
title_sort rt-qpcr assay for detection of mink astrovirus in outbreaks of diarrhea on danish mink farms
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153481/
https://www.ncbi.nlm.nih.gov/pubmed/34038467
http://dx.doi.org/10.1371/journal.pone.0252022
work_keys_str_mv AT barsøesofie rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT ullmankarin rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT leijonmikael rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT hedlundkjellolof rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT klingstromjonas rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT kraruplouiseiuel rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT andresenlars rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT quaademichellelauge rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms
AT hammerannesofie rtqpcrassayfordetectionofminkastrovirusinoutbreaksofdiarrheaondanishminkfarms