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Biofilm Removal by Reversible Shape Recovery of the Substrate

[Image: see text] Bacteria can colonize essentially any surface and form antibiotic resistant biofilms, which are multicellular structures embedded in an extracellular matrix secreted by the attached cells. To develop better biofilm control technologies, we recently demonstrated that mature biofilms...

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Autores principales: Lee, Sang Won, Carnicelli, Joseph, Getya, Dariya, Gitsov, Ivan, Phillips, K. Scott, Ren, Dacheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153534/
https://www.ncbi.nlm.nih.gov/pubmed/33822590
http://dx.doi.org/10.1021/acsami.0c20697
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author Lee, Sang Won
Carnicelli, Joseph
Getya, Dariya
Gitsov, Ivan
Phillips, K. Scott
Ren, Dacheng
author_facet Lee, Sang Won
Carnicelli, Joseph
Getya, Dariya
Gitsov, Ivan
Phillips, K. Scott
Ren, Dacheng
author_sort Lee, Sang Won
collection PubMed
description [Image: see text] Bacteria can colonize essentially any surface and form antibiotic resistant biofilms, which are multicellular structures embedded in an extracellular matrix secreted by the attached cells. To develop better biofilm control technologies, we recently demonstrated that mature biofilms can be effectively removed through on-demand shape recovery of a shape memory polymer (SMP) composed of tert-butyl acrylate (tBA). It was further demonstrated that such a dynamic substratum can sensitize the detached biofilm cells to antibiotics. However, this SMP can undergo shape change only once, limiting its application in long-term biofilm control. This motivated the present study, which aimed to prove the concept that biofilm can be effectively removed by repeated on-demand shape recovery. Reversible shape memory polymers (rSMPs) containing poly(ε-caprolactone) diisocyanatoethyl dimethacrylate (PCLDIMA) of varying molecular masses and butyl acrylate (BA) as a linker were synthesized by using benzoyl peroxide (BPO) as a thermal initiator. By comparison of several combinations of PCLDIMA of different molecular masses, a 2:1 weight ratio mixture of 2000 and 15000 g/mol PCLDIMA was the most promising because it had a shape transition (at 36.7 °C) close to body temperature. The synthesized rSMP demonstrated good reversible shape recovery and up to 94.3 ± 1.0% removal of 48 h Pseudomonas aeruginosa PAO1 biofilm cells after three consecutive shape recovery cycles. Additionally, the detached biofilm cells were found to be 5.0 ± 1.2 times more susceptible to 50 μg/mL tobramycin than the static control.
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spelling pubmed-81535342021-05-27 Biofilm Removal by Reversible Shape Recovery of the Substrate Lee, Sang Won Carnicelli, Joseph Getya, Dariya Gitsov, Ivan Phillips, K. Scott Ren, Dacheng ACS Appl Mater Interfaces [Image: see text] Bacteria can colonize essentially any surface and form antibiotic resistant biofilms, which are multicellular structures embedded in an extracellular matrix secreted by the attached cells. To develop better biofilm control technologies, we recently demonstrated that mature biofilms can be effectively removed through on-demand shape recovery of a shape memory polymer (SMP) composed of tert-butyl acrylate (tBA). It was further demonstrated that such a dynamic substratum can sensitize the detached biofilm cells to antibiotics. However, this SMP can undergo shape change only once, limiting its application in long-term biofilm control. This motivated the present study, which aimed to prove the concept that biofilm can be effectively removed by repeated on-demand shape recovery. Reversible shape memory polymers (rSMPs) containing poly(ε-caprolactone) diisocyanatoethyl dimethacrylate (PCLDIMA) of varying molecular masses and butyl acrylate (BA) as a linker were synthesized by using benzoyl peroxide (BPO) as a thermal initiator. By comparison of several combinations of PCLDIMA of different molecular masses, a 2:1 weight ratio mixture of 2000 and 15000 g/mol PCLDIMA was the most promising because it had a shape transition (at 36.7 °C) close to body temperature. The synthesized rSMP demonstrated good reversible shape recovery and up to 94.3 ± 1.0% removal of 48 h Pseudomonas aeruginosa PAO1 biofilm cells after three consecutive shape recovery cycles. Additionally, the detached biofilm cells were found to be 5.0 ± 1.2 times more susceptible to 50 μg/mL tobramycin than the static control. American Chemical Society 2021-04-06 2021-04-21 /pmc/articles/PMC8153534/ /pubmed/33822590 http://dx.doi.org/10.1021/acsami.0c20697 Text en © 2021 American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Lee, Sang Won
Carnicelli, Joseph
Getya, Dariya
Gitsov, Ivan
Phillips, K. Scott
Ren, Dacheng
Biofilm Removal by Reversible Shape Recovery of the Substrate
title Biofilm Removal by Reversible Shape Recovery of the Substrate
title_full Biofilm Removal by Reversible Shape Recovery of the Substrate
title_fullStr Biofilm Removal by Reversible Shape Recovery of the Substrate
title_full_unstemmed Biofilm Removal by Reversible Shape Recovery of the Substrate
title_short Biofilm Removal by Reversible Shape Recovery of the Substrate
title_sort biofilm removal by reversible shape recovery of the substrate
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153534/
https://www.ncbi.nlm.nih.gov/pubmed/33822590
http://dx.doi.org/10.1021/acsami.0c20697
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