Cargando…

Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi

Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypoma...

Descripción completa

Detalles Bibliográficos
Autores principales: Retana Moreira, Lissette, Prescilla-Ledezma, Alexa, Cornet-Gomez, Alberto, Linares, Fátima, Jódar-Reyes, Ana Belén, Fernandez, Jorge, Ibarrola Vannucci, Ana Karina, De Pablos, Luis Miguel, Osuna, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153575/
https://www.ncbi.nlm.nih.gov/pubmed/34068436
http://dx.doi.org/10.3390/ijms22105183
_version_ 1783698829572308992
author Retana Moreira, Lissette
Prescilla-Ledezma, Alexa
Cornet-Gomez, Alberto
Linares, Fátima
Jódar-Reyes, Ana Belén
Fernandez, Jorge
Ibarrola Vannucci, Ana Karina
De Pablos, Luis Miguel
Osuna, Antonio
author_facet Retana Moreira, Lissette
Prescilla-Ledezma, Alexa
Cornet-Gomez, Alberto
Linares, Fátima
Jódar-Reyes, Ana Belén
Fernandez, Jorge
Ibarrola Vannucci, Ana Karina
De Pablos, Luis Miguel
Osuna, Antonio
author_sort Retana Moreira, Lissette
collection PubMed
description Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of Trypanosoma cruzi, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential centrifugation and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), electron microscopy and atomic force microscopy (AFM). Measurements of zeta-potential were also included. Results show marked differences in the surface molecular cargos of EVs between both stages, with a noteworthy expansion of all groups of trans-sialidase proteins in trypomastigote’s EVs. In contrast, chromosomal locations of trans-sialidases of EVs of epimastigotes were dramatically reduced and restricted to subtelomeric regions, indicating a possible regulatable expression of these proteins between both stages of the parasite. Regarding mechanical properties, EVs of trypomastigotes showed higher adhesion compared to the EVs of epimastigotes. These findings demonstrate the remarkable surface remodeling throughout the life cycle of T. cruzi, which shapes the physicochemical composition of the extracellular vesicles and could have an impact in the ability of these vesicles to participate in cell communication in completely different niches of infection.
format Online
Article
Text
id pubmed-8153575
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81535752021-05-27 Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi Retana Moreira, Lissette Prescilla-Ledezma, Alexa Cornet-Gomez, Alberto Linares, Fátima Jódar-Reyes, Ana Belén Fernandez, Jorge Ibarrola Vannucci, Ana Karina De Pablos, Luis Miguel Osuna, Antonio Int J Mol Sci Article Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of Trypanosoma cruzi, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential centrifugation and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), electron microscopy and atomic force microscopy (AFM). Measurements of zeta-potential were also included. Results show marked differences in the surface molecular cargos of EVs between both stages, with a noteworthy expansion of all groups of trans-sialidase proteins in trypomastigote’s EVs. In contrast, chromosomal locations of trans-sialidases of EVs of epimastigotes were dramatically reduced and restricted to subtelomeric regions, indicating a possible regulatable expression of these proteins between both stages of the parasite. Regarding mechanical properties, EVs of trypomastigotes showed higher adhesion compared to the EVs of epimastigotes. These findings demonstrate the remarkable surface remodeling throughout the life cycle of T. cruzi, which shapes the physicochemical composition of the extracellular vesicles and could have an impact in the ability of these vesicles to participate in cell communication in completely different niches of infection. MDPI 2021-05-13 /pmc/articles/PMC8153575/ /pubmed/34068436 http://dx.doi.org/10.3390/ijms22105183 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Retana Moreira, Lissette
Prescilla-Ledezma, Alexa
Cornet-Gomez, Alberto
Linares, Fátima
Jódar-Reyes, Ana Belén
Fernandez, Jorge
Ibarrola Vannucci, Ana Karina
De Pablos, Luis Miguel
Osuna, Antonio
Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
title Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
title_full Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
title_fullStr Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
title_full_unstemmed Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
title_short Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
title_sort biophysical and biochemical comparison of extracellular vesicles produced by infective and non-infective stages of trypanosoma cruzi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153575/
https://www.ncbi.nlm.nih.gov/pubmed/34068436
http://dx.doi.org/10.3390/ijms22105183
work_keys_str_mv AT retanamoreiralissette biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT prescillaledezmaalexa biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT cornetgomezalberto biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT linaresfatima biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT jodarreyesanabelen biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT fernandezjorge biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT ibarrolavannuccianakarina biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT depablosluismiguel biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi
AT osunaantonio biophysicalandbiochemicalcomparisonofextracellularvesiclesproducedbyinfectiveandnoninfectivestagesoftrypanosomacruzi