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Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi
Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypoma...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153575/ https://www.ncbi.nlm.nih.gov/pubmed/34068436 http://dx.doi.org/10.3390/ijms22105183 |
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author | Retana Moreira, Lissette Prescilla-Ledezma, Alexa Cornet-Gomez, Alberto Linares, Fátima Jódar-Reyes, Ana Belén Fernandez, Jorge Ibarrola Vannucci, Ana Karina De Pablos, Luis Miguel Osuna, Antonio |
author_facet | Retana Moreira, Lissette Prescilla-Ledezma, Alexa Cornet-Gomez, Alberto Linares, Fátima Jódar-Reyes, Ana Belén Fernandez, Jorge Ibarrola Vannucci, Ana Karina De Pablos, Luis Miguel Osuna, Antonio |
author_sort | Retana Moreira, Lissette |
collection | PubMed |
description | Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of Trypanosoma cruzi, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential centrifugation and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), electron microscopy and atomic force microscopy (AFM). Measurements of zeta-potential were also included. Results show marked differences in the surface molecular cargos of EVs between both stages, with a noteworthy expansion of all groups of trans-sialidase proteins in trypomastigote’s EVs. In contrast, chromosomal locations of trans-sialidases of EVs of epimastigotes were dramatically reduced and restricted to subtelomeric regions, indicating a possible regulatable expression of these proteins between both stages of the parasite. Regarding mechanical properties, EVs of trypomastigotes showed higher adhesion compared to the EVs of epimastigotes. These findings demonstrate the remarkable surface remodeling throughout the life cycle of T. cruzi, which shapes the physicochemical composition of the extracellular vesicles and could have an impact in the ability of these vesicles to participate in cell communication in completely different niches of infection. |
format | Online Article Text |
id | pubmed-8153575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81535752021-05-27 Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi Retana Moreira, Lissette Prescilla-Ledezma, Alexa Cornet-Gomez, Alberto Linares, Fátima Jódar-Reyes, Ana Belén Fernandez, Jorge Ibarrola Vannucci, Ana Karina De Pablos, Luis Miguel Osuna, Antonio Int J Mol Sci Article Extracellular vesicles (EVs) are small lipid vesicles released by either any prokaryotic or eukaryotic cell, or both, with a biological role in cell-to-cell communication. In this work, we characterize the proteomes and nanomechanical properties of EVs released by tissue-culture cell-derived trypomastigotes (mammalian infective stage; (TCT)) and epimastigotes (insect stage; (E)) of Trypanosoma cruzi, the etiologic agent of Chagas disease. EVs of each stage were isolated by differential centrifugation and analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS), dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), electron microscopy and atomic force microscopy (AFM). Measurements of zeta-potential were also included. Results show marked differences in the surface molecular cargos of EVs between both stages, with a noteworthy expansion of all groups of trans-sialidase proteins in trypomastigote’s EVs. In contrast, chromosomal locations of trans-sialidases of EVs of epimastigotes were dramatically reduced and restricted to subtelomeric regions, indicating a possible regulatable expression of these proteins between both stages of the parasite. Regarding mechanical properties, EVs of trypomastigotes showed higher adhesion compared to the EVs of epimastigotes. These findings demonstrate the remarkable surface remodeling throughout the life cycle of T. cruzi, which shapes the physicochemical composition of the extracellular vesicles and could have an impact in the ability of these vesicles to participate in cell communication in completely different niches of infection. MDPI 2021-05-13 /pmc/articles/PMC8153575/ /pubmed/34068436 http://dx.doi.org/10.3390/ijms22105183 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Retana Moreira, Lissette Prescilla-Ledezma, Alexa Cornet-Gomez, Alberto Linares, Fátima Jódar-Reyes, Ana Belén Fernandez, Jorge Ibarrola Vannucci, Ana Karina De Pablos, Luis Miguel Osuna, Antonio Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi |
title | Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi |
title_full | Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi |
title_fullStr | Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi |
title_full_unstemmed | Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi |
title_short | Biophysical and Biochemical Comparison of Extracellular Vesicles Produced by Infective and Non-Infective Stages of Trypanosoma cruzi |
title_sort | biophysical and biochemical comparison of extracellular vesicles produced by infective and non-infective stages of trypanosoma cruzi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153575/ https://www.ncbi.nlm.nih.gov/pubmed/34068436 http://dx.doi.org/10.3390/ijms22105183 |
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