Cargando…

Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array

Dynamic mapping of the cell-generated force of cardiomyocytes will help provide an intrinsic understanding of the heart. However, a real-time, dynamic, and high-resolution mapping of the force distribution across a single living cell remains a challenge. Here, we established a force mapping method b...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Qiang, Peng, Mingzeng, Liu, Zhuo, Li, Shuyu, Han, Rongcheng, Ouyang, Han, Fan, Yubo, Pan, Caofeng, Hu, Weiguo, Zhai, Junyi, Li, Zhou, Wang, Zhong Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153726/
https://www.ncbi.nlm.nih.gov/pubmed/34039600
http://dx.doi.org/10.1126/sciadv.abe7738
_version_ 1783698862945337344
author Zheng, Qiang
Peng, Mingzeng
Liu, Zhuo
Li, Shuyu
Han, Rongcheng
Ouyang, Han
Fan, Yubo
Pan, Caofeng
Hu, Weiguo
Zhai, Junyi
Li, Zhou
Wang, Zhong Lin
author_facet Zheng, Qiang
Peng, Mingzeng
Liu, Zhuo
Li, Shuyu
Han, Rongcheng
Ouyang, Han
Fan, Yubo
Pan, Caofeng
Hu, Weiguo
Zhai, Junyi
Li, Zhou
Wang, Zhong Lin
author_sort Zheng, Qiang
collection PubMed
description Dynamic mapping of the cell-generated force of cardiomyocytes will help provide an intrinsic understanding of the heart. However, a real-time, dynamic, and high-resolution mapping of the force distribution across a single living cell remains a challenge. Here, we established a force mapping method based on a “light nano-antenna” array with the use of piezo-phototronic effect. A spatial resolution of 800 nm and a temporal resolution of 333 ms have been demonstrated for force mapping. The dynamic mapping of cell force of live cardiomyocytes was directly derived by locating the antennas’ positions and quantifying the light intensities of the piezo-phototronic light nano-antenna array. This study presents a rapid and ultrahigh-resolution methodology for the fundamental study of cardiomyocyte behavior at the cell or subcellular level. It can provide valuable information about disease detection, drug screening, and tissue engineering for heart-related studies.
format Online
Article
Text
id pubmed-8153726
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Association for the Advancement of Science
record_format MEDLINE/PubMed
spelling pubmed-81537262021-06-07 Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array Zheng, Qiang Peng, Mingzeng Liu, Zhuo Li, Shuyu Han, Rongcheng Ouyang, Han Fan, Yubo Pan, Caofeng Hu, Weiguo Zhai, Junyi Li, Zhou Wang, Zhong Lin Sci Adv Research Articles Dynamic mapping of the cell-generated force of cardiomyocytes will help provide an intrinsic understanding of the heart. However, a real-time, dynamic, and high-resolution mapping of the force distribution across a single living cell remains a challenge. Here, we established a force mapping method based on a “light nano-antenna” array with the use of piezo-phototronic effect. A spatial resolution of 800 nm and a temporal resolution of 333 ms have been demonstrated for force mapping. The dynamic mapping of cell force of live cardiomyocytes was directly derived by locating the antennas’ positions and quantifying the light intensities of the piezo-phototronic light nano-antenna array. This study presents a rapid and ultrahigh-resolution methodology for the fundamental study of cardiomyocyte behavior at the cell or subcellular level. It can provide valuable information about disease detection, drug screening, and tissue engineering for heart-related studies. American Association for the Advancement of Science 2021-05-26 /pmc/articles/PMC8153726/ /pubmed/34039600 http://dx.doi.org/10.1126/sciadv.abe7738 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Zheng, Qiang
Peng, Mingzeng
Liu, Zhuo
Li, Shuyu
Han, Rongcheng
Ouyang, Han
Fan, Yubo
Pan, Caofeng
Hu, Weiguo
Zhai, Junyi
Li, Zhou
Wang, Zhong Lin
Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
title Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
title_full Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
title_fullStr Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
title_full_unstemmed Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
title_short Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
title_sort dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153726/
https://www.ncbi.nlm.nih.gov/pubmed/34039600
http://dx.doi.org/10.1126/sciadv.abe7738
work_keys_str_mv AT zhengqiang dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT pengmingzeng dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT liuzhuo dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT lishuyu dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT hanrongcheng dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT ouyanghan dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT fanyubo dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT pancaofeng dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT huweiguo dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT zhaijunyi dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT lizhou dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray
AT wangzhonglin dynamicrealtimeimagingoflivingcelltractionforcebypiezophototroniclightnanoantennaarray