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Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films
[Image: see text] Platelet-surface interaction is of paramount importance in biomedical applications as well as in vitro studies. However, controlling platelet-surface activation is challenging and still requires more effort as they activate immediately when contacting with any nonphysiological surf...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153948/ https://www.ncbi.nlm.nih.gov/pubmed/34056249 http://dx.doi.org/10.1021/acsomega.1c00764 |
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author | Apte, Gurunath Lindenbauer, Annerose Schemberg, Jörg Rothe, Holger Nguyen, Thi-Huong |
author_facet | Apte, Gurunath Lindenbauer, Annerose Schemberg, Jörg Rothe, Holger Nguyen, Thi-Huong |
author_sort | Apte, Gurunath |
collection | PubMed |
description | [Image: see text] Platelet-surface interaction is of paramount importance in biomedical applications as well as in vitro studies. However, controlling platelet-surface activation is challenging and still requires more effort as they activate immediately when contacting with any nonphysiological surface. As hydrogels are highly biocompatible, in this study, we developed agarose and gelatin-based hydrogel films to inhibit platelet-surface adhesion. We found promising agarose films that exhibit higher surface wettability, better controlled-swelling properties, and greater stiffness compared to gelatin, resulting in a strong reduction of platelet adhesion. Mechanical properties and surface wettability of the hydrogel films were varied by adding magnetite (Fe(3)O(4)) nanoparticles. While all of the films prevented platelet spreading, films formed by agarose and its nanocomposite repelled platelets and inhibited platelet adhesion and activation stronger than those of gelatin. Our results showed that platelet-surface activation is modulated by controlling the properties of the films underneath platelets and that the bioinert agarose can be potentially translated to the development of platelet storage and other medical applications. |
format | Online Article Text |
id | pubmed-8153948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-81539482021-05-27 Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films Apte, Gurunath Lindenbauer, Annerose Schemberg, Jörg Rothe, Holger Nguyen, Thi-Huong ACS Omega [Image: see text] Platelet-surface interaction is of paramount importance in biomedical applications as well as in vitro studies. However, controlling platelet-surface activation is challenging and still requires more effort as they activate immediately when contacting with any nonphysiological surface. As hydrogels are highly biocompatible, in this study, we developed agarose and gelatin-based hydrogel films to inhibit platelet-surface adhesion. We found promising agarose films that exhibit higher surface wettability, better controlled-swelling properties, and greater stiffness compared to gelatin, resulting in a strong reduction of platelet adhesion. Mechanical properties and surface wettability of the hydrogel films were varied by adding magnetite (Fe(3)O(4)) nanoparticles. While all of the films prevented platelet spreading, films formed by agarose and its nanocomposite repelled platelets and inhibited platelet adhesion and activation stronger than those of gelatin. Our results showed that platelet-surface activation is modulated by controlling the properties of the films underneath platelets and that the bioinert agarose can be potentially translated to the development of platelet storage and other medical applications. American Chemical Society 2021-04-13 /pmc/articles/PMC8153948/ /pubmed/34056249 http://dx.doi.org/10.1021/acsomega.1c00764 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Apte, Gurunath Lindenbauer, Annerose Schemberg, Jörg Rothe, Holger Nguyen, Thi-Huong Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films |
title | Controlling Surface-Induced Platelet Activation by
Agarose and Gelatin-Based Hydrogel Films |
title_full | Controlling Surface-Induced Platelet Activation by
Agarose and Gelatin-Based Hydrogel Films |
title_fullStr | Controlling Surface-Induced Platelet Activation by
Agarose and Gelatin-Based Hydrogel Films |
title_full_unstemmed | Controlling Surface-Induced Platelet Activation by
Agarose and Gelatin-Based Hydrogel Films |
title_short | Controlling Surface-Induced Platelet Activation by
Agarose and Gelatin-Based Hydrogel Films |
title_sort | controlling surface-induced platelet activation by
agarose and gelatin-based hydrogel films |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153948/ https://www.ncbi.nlm.nih.gov/pubmed/34056249 http://dx.doi.org/10.1021/acsomega.1c00764 |
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