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OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408
microRNAs (miRNAs) are promising targets for crop improvement of complex agricultural traits. Coordinated activity between/among different miRNAs may fine-tune specific developmental processes in diverse organisms. Grain size is a main factor determining rice (Oryza sativa L.) crop yield, but the ne...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154042/ https://www.ncbi.nlm.nih.gov/pubmed/33620493 http://dx.doi.org/10.1093/plphys/kiab084 |
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author | Yang, Xiaofang Zhao, Xiaoling Dai, Zhengyan Ma, Feilong Miao, Xuexia Shi, Zhenying |
author_facet | Yang, Xiaofang Zhao, Xiaoling Dai, Zhengyan Ma, Feilong Miao, Xuexia Shi, Zhenying |
author_sort | Yang, Xiaofang |
collection | PubMed |
description | microRNAs (miRNAs) are promising targets for crop improvement of complex agricultural traits. Coordinated activity between/among different miRNAs may fine-tune specific developmental processes in diverse organisms. Grain size is a main factor determining rice (Oryza sativa L.) crop yield, but the network of miRNAs influencing this trait remains uncharacterized. Here we show that sequestering OsmiR396 through target mimicry (MIM396) can substantially increase grain size in several japonica and indica rice subspecies and in plants with excessive tillers and a high panicle density. Thus, OsmiR396 has a major role related to the regulation of rice grain size. The grain shape of Growth Regulating Factor8 (OsGRF8)-overexpressing transgenic plants was most similar to that of MIM396 plants, suggesting OsGRF8 is a major mediator of OsmiR396 in grain size regulation. A miRNA microarray analysis revealed changes to the expression of many miRNAs, including OsmiR408, in the MIM396 plants. Analyses of gene expression patterns and functions indicated OsmiR408 is an embryo-specific miRNA that positively regulates grain size. Silencing OsmiR408 expression (miR408KO) using CRISPR technology resulted in small grains. Moreover, we revealed the direct regulatory effects of OsGRF8 on OsMIR408 expression. A genetic analysis further showed that the large-grain phenotype of MIM396 plants could be complemented by miR408KO. Also, several hormone signaling pathways might be involved in the OsmiR396/GRF-meditated grain size regulation. Our findings suggest that genetic regulatory networks comprising various miRNAs, such as OsmiR396 and OsmiR408, may be crucial for controlling rice grain size. Furthermore, the OsmiR396/GRF module may be important for breeding new high-yielding rice varieties. |
format | Online Article Text |
id | pubmed-8154042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-81540422021-05-28 OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 Yang, Xiaofang Zhao, Xiaoling Dai, Zhengyan Ma, Feilong Miao, Xuexia Shi, Zhenying Plant Physiol Regular Issue microRNAs (miRNAs) are promising targets for crop improvement of complex agricultural traits. Coordinated activity between/among different miRNAs may fine-tune specific developmental processes in diverse organisms. Grain size is a main factor determining rice (Oryza sativa L.) crop yield, but the network of miRNAs influencing this trait remains uncharacterized. Here we show that sequestering OsmiR396 through target mimicry (MIM396) can substantially increase grain size in several japonica and indica rice subspecies and in plants with excessive tillers and a high panicle density. Thus, OsmiR396 has a major role related to the regulation of rice grain size. The grain shape of Growth Regulating Factor8 (OsGRF8)-overexpressing transgenic plants was most similar to that of MIM396 plants, suggesting OsGRF8 is a major mediator of OsmiR396 in grain size regulation. A miRNA microarray analysis revealed changes to the expression of many miRNAs, including OsmiR408, in the MIM396 plants. Analyses of gene expression patterns and functions indicated OsmiR408 is an embryo-specific miRNA that positively regulates grain size. Silencing OsmiR408 expression (miR408KO) using CRISPR technology resulted in small grains. Moreover, we revealed the direct regulatory effects of OsGRF8 on OsMIR408 expression. A genetic analysis further showed that the large-grain phenotype of MIM396 plants could be complemented by miR408KO. Also, several hormone signaling pathways might be involved in the OsmiR396/GRF-meditated grain size regulation. Our findings suggest that genetic regulatory networks comprising various miRNAs, such as OsmiR396 and OsmiR408, may be crucial for controlling rice grain size. Furthermore, the OsmiR396/GRF module may be important for breeding new high-yielding rice varieties. Oxford University Press 2021-02-23 /pmc/articles/PMC8154042/ /pubmed/33620493 http://dx.doi.org/10.1093/plphys/kiab084 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Regular Issue Yang, Xiaofang Zhao, Xiaoling Dai, Zhengyan Ma, Feilong Miao, Xuexia Shi, Zhenying OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 |
title | OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 |
title_full | OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 |
title_fullStr | OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 |
title_full_unstemmed | OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 |
title_short | OsmiR396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific miR408 |
title_sort | osmir396/growth regulating factor modulate rice grain size through direct regulation of embryo-specific mir408 |
topic | Regular Issue |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154042/ https://www.ncbi.nlm.nih.gov/pubmed/33620493 http://dx.doi.org/10.1093/plphys/kiab084 |
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