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Noncovalent Protein–Pseudorotaxane Assembly Incorporating an Extended Arm Calix[8]arene with α-Helical Recognition Properties
[Image: see text] Water-soluble, anionic calix[n]arenes are useful receptors for protein recognition and assembly. For example, sulfonato-calix[8]arene (sclx(8)) can encapsulate proteins and direct their assembly into porous frameworks. In this work, we turned our attention to an “extended arm” cali...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154262/ https://www.ncbi.nlm.nih.gov/pubmed/34054353 http://dx.doi.org/10.1021/acs.cgd.0c01717 |
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author | Mockler, Niamh M. Ramberg, Kiefer O. Guagnini, Francesca Raston, Colin L. Crowley, Peter B. |
author_facet | Mockler, Niamh M. Ramberg, Kiefer O. Guagnini, Francesca Raston, Colin L. Crowley, Peter B. |
author_sort | Mockler, Niamh M. |
collection | PubMed |
description | [Image: see text] Water-soluble, anionic calix[n]arenes are useful receptors for protein recognition and assembly. For example, sulfonato-calix[8]arene (sclx(8)) can encapsulate proteins and direct their assembly into porous frameworks. In this work, we turned our attention to an “extended arm” calixarene with 16 phenyl rings. We hypothesized that this larger receptor would have increased capacity for protein masking/encapsulation. A cocrystal structure of p-benzyl-sulfonato-calix[8]arene (b-sclx(8)) and cytochrome c (cyt c) revealed a surprising assembly. A pseudorotaxane comprising a stack of three b-sclx(8) molecules threaded by polyethylene glycol (PEG) was bound to the protein. The trimeric b-sclx(8) stack, a tubelike structure with a highly charged surface, mediated assembly via a new mode of protein recognition. The calixarene stack presents four hydrophobic grooves, each of which binds to one cyt c by accommodating the N-terminal α-helix. This unprecedented binding mode suggests new possibilities for supramolecular protein chemistry. |
format | Online Article Text |
id | pubmed-8154262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-81542622021-05-27 Noncovalent Protein–Pseudorotaxane Assembly Incorporating an Extended Arm Calix[8]arene with α-Helical Recognition Properties Mockler, Niamh M. Ramberg, Kiefer O. Guagnini, Francesca Raston, Colin L. Crowley, Peter B. Cryst Growth Des [Image: see text] Water-soluble, anionic calix[n]arenes are useful receptors for protein recognition and assembly. For example, sulfonato-calix[8]arene (sclx(8)) can encapsulate proteins and direct their assembly into porous frameworks. In this work, we turned our attention to an “extended arm” calixarene with 16 phenyl rings. We hypothesized that this larger receptor would have increased capacity for protein masking/encapsulation. A cocrystal structure of p-benzyl-sulfonato-calix[8]arene (b-sclx(8)) and cytochrome c (cyt c) revealed a surprising assembly. A pseudorotaxane comprising a stack of three b-sclx(8) molecules threaded by polyethylene glycol (PEG) was bound to the protein. The trimeric b-sclx(8) stack, a tubelike structure with a highly charged surface, mediated assembly via a new mode of protein recognition. The calixarene stack presents four hydrophobic grooves, each of which binds to one cyt c by accommodating the N-terminal α-helix. This unprecedented binding mode suggests new possibilities for supramolecular protein chemistry. American Chemical Society 2021-02-08 2021-03-03 /pmc/articles/PMC8154262/ /pubmed/34054353 http://dx.doi.org/10.1021/acs.cgd.0c01717 Text en © 2021 American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Mockler, Niamh M. Ramberg, Kiefer O. Guagnini, Francesca Raston, Colin L. Crowley, Peter B. Noncovalent Protein–Pseudorotaxane Assembly Incorporating an Extended Arm Calix[8]arene with α-Helical Recognition Properties |
title | Noncovalent Protein–Pseudorotaxane Assembly
Incorporating an Extended Arm Calix[8]arene with α-Helical
Recognition Properties |
title_full | Noncovalent Protein–Pseudorotaxane Assembly
Incorporating an Extended Arm Calix[8]arene with α-Helical
Recognition Properties |
title_fullStr | Noncovalent Protein–Pseudorotaxane Assembly
Incorporating an Extended Arm Calix[8]arene with α-Helical
Recognition Properties |
title_full_unstemmed | Noncovalent Protein–Pseudorotaxane Assembly
Incorporating an Extended Arm Calix[8]arene with α-Helical
Recognition Properties |
title_short | Noncovalent Protein–Pseudorotaxane Assembly
Incorporating an Extended Arm Calix[8]arene with α-Helical
Recognition Properties |
title_sort | noncovalent protein–pseudorotaxane assembly
incorporating an extended arm calix[8]arene with α-helical
recognition properties |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154262/ https://www.ncbi.nlm.nih.gov/pubmed/34054353 http://dx.doi.org/10.1021/acs.cgd.0c01717 |
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